Transcriptional Repression of Caveolin-1 (CAV1) Gene Expression by GATA-6 in Bladder Smooth Muscle Hypertrophy in Mice and Human Beings

Show simple item record

dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author BOOPATHI, Ettickan
GOMES, Cristiano Mendes FMUSP-HC
GOLDFARB, Robert
JOHN, Mary
SRINIVASAN, Vittala Gopal
ALANZI, Jaber
MALKOWICZ, S. Bruce
KATHURIA, Hasmeena
ZDERIC, Stephen A.
WEIN, Alan J.
CHACKO, Samuel
dc.date.issued 2011
dc.identifier.citation AMERICAN JOURNAL OF PATHOLOGY, v.178, n.5, p.2236-2251, 2011
dc.identifier.issn 0002-9440
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/23992
dc.description.abstract Hypertrophy occurs in urinary bladder wall smooth muscle (BSM) in men with partial bladder outlet obstruction (PBOO) caused by benign prostatic hyperplasia (BPH) and in animal models of PBOO. Hypertrophied BSM from the rabbit model exhibits down-regulation of caveolin-1, a structural and functional protein of caveolae that function as signaling platforms to mediate interaction between receptor proteins and adaptor and effector molecules to regulate signal generation, amplification, and diversification. Caveolin-1 expression is diminished in PBOO-induced BSM hypertrophy in mice and in men with BPH. The proximal promoter of the human and mouse caveolin-1 (CAV1) gene was characterized, and it was observed that the transcription factor GATA-6 binds this promoter, causing reduced expression of caveolin-1. Furthermore, caveolin-1 expression levels inversely correlate with the abundance of GATA-6 in BSM hypertrophy in mice and human beings. Silencing of GATA6 gene expression up-regulates caveolin-1 expression, whereas overexpression of GATA-6 protein sustains the transcriptional repression of caveolin-1 in bladder smooth muscle cells. Together, these data suggest that GATA-6 acts as a transcriptional repressor of CAV1 gene expression in PBOO-induced BSM hypertrophy in men and mice. GATA-6-induced transcriptional repression represents a new regulatory mechanism of CAV1 gene expression in pathologic BSM, and may serve as a target for new therapy for BPH-induced bladder dysfunction in aging men. (Am J Pathol 2011, 178:2236-2251; DOI 10.1016/j.ajpath.2011.01.038)
dc.description.sponsorship · George O'Brien Urology Research Center [P50 DK052620]
dc.language.iso eng
dc.publisher ELSEVIER SCIENCE INC
dc.relation.ispartof American Journal of Pathology
dc.rights restrictedAccess
dc.subject.other urinary-tract function; outlet obstruction; cell-lines; continence-society; binding-proteins; ets proteins; lipid rafts; map kinase; in-vivo; differentiation
dc.title Transcriptional Repression of Caveolin-1 (CAV1) Gene Expression by GATA-6 in Bladder Smooth Muscle Hypertrophy in Mice and Human Beings
dc.type article
dc.rights.holder Copyright ELSEVIER SCIENCE INC
dc.description.group LIM/55
dc.identifier.doi 10.1016/j.ajpath.2011.01.038
dc.identifier.pmid 21514437
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author GOMES, Cristiano Mendes:HC:IOT
hcfmusp.author.external · KATHURIA, Hasmeena:Boston Univ, Sch Med, Boston, MA 02118 USA
· ZDERIC, Stephen A.:Childrens Hosp Philadelphia, Dept Urol, Philadelphia, PA 19104 USA
· CHACKO, Samuel:Univ Penn, Glenolden Res Lab, Div Urol, Glenolden, PA 19036 USA; Univ Penn, Dept Pathobiol, Glenolden, PA 19036 USA
hcfmusp.origem.id WOS:000298306800030
hcfmusp.origem.id 2-s2.0-79959256001
hcfmusp.publisher.city NEW YORK
hcfmusp.publisher.country USA
hcfmusp.relation.reference · Morrisey EE, 1996, DEV BIOL, V177, P309, DOI 10.1006/dbio.1996.0165
· Masternak K, 2000, GENE DEV, V14, P1156
· Williams TM, 2005, AM J PHYSIOL-CELL PH, V288, pC494, DOI 10.1152/ajpcell.00458.2004
· Cartharius K, 2005, BIOINFORMATICS, V21, P2933, DOI 10.1093/bioinformatics/bti473
· Abrams P, 1999, BJU INT, V84, P14
· Fong A, 2003, AM J CLIN PATHOL, V120, P93, DOI 10.1309/292NHAYNWAVREJ37
· Rahman A, 2009, ACTA PHYSIOL, V195, P231, DOI 10.1111/j.1748-1716.2008.01907.x
· Stehr M, 2003, J UROLOGY, V169, P1165, DOI 10.1097/01.ju.0000041501.01323.b9
· Kunze E, 2006, INT J MOL MED, V17, P3
· Drab M, 2001, SCIENCE, V293, P2449, DOI 10.1126/science.1062688
· Galbiati F, 2001, CELL, V106, P403, DOI 10.1016/S0092-8674(01)00472-X
· Rajjayabun PH, 2001, UROLOGY, V58, P811, DOI 10.1016/S0090-4295(01)01337-1
· Bing W, 2003, AM J PHYSIOL-RENAL, V285, pF990, DOI 10.1152/ajprenal.00378.2002
· Stewart SA, 2003, RNA, V9, P493, DOI 10.1261/rna.2192803
· Cohen AW, 2003, AM J PHYSIOL-CELL PH, V284, pC457, DOI 10.1152/ajpcell.00380.2002
· Lepore JJ, 2005, ARTERIOSCL THROM VAS, V25, P309, DOI 10.1161/01.ATV.0000152725.76020.3c
· Williams TM, 2004, J BIOL CHEM, V279, P51630, DOI 10.1074/jbc.M409214200
· Polyak E, 2009, J UROLOGY, V182, P2497, DOI 10.1016/j.juro.2009.07.011
· KOLESKE AJ, 1995, P NATL ACAD SCI USA, V92, P1381, DOI 10.1073/pnas.92.5.1381
· Chang DF, 2003, DEV CELL, V4, P107, DOI 10.1016/S1534-5807(02)00396-9
· KO LJ, 1993, MOL CELL BIOL, V13, P4011
· Morrisey EE, 1997, DEV BIOL, V183, P21, DOI 10.1006/dbio.1996.8485
· Abrams P, 2002, NEUROUROL URODYNAM, V21, P167, DOI 10.1002/nau.10052
· Patel HH, 2007, FASEB J, V21, P2970, DOI 10.1096/fj.07-8424com
· Burch JBE, 2005, SEMIN CELL DEV BIOL, V16, P71, DOI 10.1016/j.semcdb.2004.10.002
· Cao GW, 2003, AM J PATHOL, V162, P1241, DOI 10.1016/S0002-9440(10)63920-X
· Liang QR, 2001, J BIOL CHEM, V276, P30245, DOI 10.1074/jbc.M102174200
· Sakai Y, 1998, BIOCHEM BIOPH RES CO, V250, P682, DOI 10.1006/bbrc.1998.9374
· DiSanto ME, 2003, AM J PHYSIOL-CELL PH, V285, pC1397, DOI 10.1152/ajpcell.00513.2002
· Okamoto T, 1998, J BIOL CHEM, V273, P5419, DOI 10.1074/jbc.273.10.5419
· Fielding CJ, 2003, BBA-BIOMEMBRANES, V1610, P219, DOI 10.1016/S0005-2736(03)00020-8
· SCHREIBER E, 1989, NUCLEIC ACIDS RES, V17, P6419, DOI 10.1093/nar/17.15.6419
· Shirvani S, 2006, BIOCHEM BIOPH RES CO, V339, P151, DOI 10.1016/j.bbrc.2005.10.190
· DIGNAM JD, 1983, NUCLEIC ACIDS RES, V11, P1475, DOI 10.1093/nar/11.5.1475
· Abrams P, 1997, NEUROUROL URODYNAM, V16, P79
· ORKIN SH, 1992, BLOOD, V80, P575
· Sloan KA, 2009, CARCINOGENESIS, V30, P1433, DOI 10.1093/carcin/bgp129
· Austin JC, 2004, J UROLOGY, V172, P1524, DOI 10.1097/01.ju.0000138045.61378.96
· Levin RM, 2000, NEUROUROL URODYNAM, V19, P609, DOI 10.1002/1520-6777(2000)19:5<609::AID-NAU7>3.0.CO;2-H
· Kathuria H, 2004, J BIOL CHEM, V279, P30028, DOI 10.1074/jbc.M402236200
· Molkentin JD, 2000, J BIOL CHEM, V275, P38949, DOI 10.1074/jbc.R000029200
· Hanke M, 2007, CLIN CHEM, V53, P2070, DOI 10.1373/clinchem.2007.091363
· Bergdahl A, 2004, CAN J PHYSIOL PHARM, V82, P289, DOI 10.1139/Y04-033
· Bist A, 1997, P NATL ACAD SCI USA, V94, P10693, DOI 10.1073/pnas.94.20.10693
· BIST J, 2000, BIOCHEMISTRY-US, V39, P1966
· Cho DS, 2008, J KOREAN MED SCI, V23, P296, DOI 10.3346/jkms.2008.23.2.296
· COUET S, 1997, J BIOL CHEM, V272, P6525
· Griffiths D, 1997, NEUROUROL URODYNAM, V16, P1, DOI 10.1002/(SICI)1520-6777(1997)16:1<1::AID-NAU1>3.0.CO;2-I
· KADONAGA JT, 1986, P NATL ACAD SCI USA, V83, P5889, DOI 10.1073/pnas.83.16.5889
· Kanematsu A, 2007, AM J PHYSIOL-CELL PH, V293, pC1093, DOI 10.1152/ajpcell.00225.2007
· Lee SJ, 2008, ONCOL REP, V19, P1007
· Lee YH, 2001, EXP PHYSIOL, V86, P283, DOI 10.1113/eph8602184
· Lipardi C, 1998, J CELL BIOL, V140, P617, DOI 10.1083/jcb.140.3.617
· Okada T, 1998, NUCLEIC ACIDS RES, V26, P1947, DOI 10.1093/nar/26.8.1947
· ONEILL LP, 1995, EMBO J, V14, P3946
· Razani B, 2001, J CLIN INVEST, V108, P1553, DOI 10.1172/JCI14611
· Roy UKB, 2008, MOL CARCINOGEN, V47, P947, DOI 10.1002/mc.20451
· Stehr M, 2004, J UROLOGY, V172, P2451, DOI 10.1097/01.ju.0000138084.53577.ca
· THOMAS S, 2010, CANCER RES, V71, P832
· van den Heuvel APJ, 2005, BIOCHEM J, V385, P795
· WEIN AJ, 1998, CAMPBELLS UROLOGY, P917
· Woodman SE, 2004, J UROLOGY, V171, P950, DOI 10.1097/01.ju.0000105102.72295.b8
· Yin F, 2005, J BIOL CHEM, V280, P4745
· ZHANG E, 2000, AM J PATHOL, V164, P601
dc.description.index MEDLINE
hcfmusp.citation.scopus 24
hcfmusp.citation.wos 23
hcfmusp.affiliation.country Brasil
hcfmusp.affiliation.country Estados Unidos


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace



Browse

My Account

Statistics