Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/24307
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorMARCONDES, Rodrigo Rodrigues-
dc.contributor.authorCARVALHO, Katia Candido-
dc.contributor.authorGIANNOCCO, Gisele-
dc.contributor.authorDUARTE, Daniele Coelho-
dc.contributor.authorGARCIA, Natalia-
dc.contributor.authorSOARES-JUNIOR, Jose Maria-
dc.contributor.authorSILVA, Ismael Dale Cotrim Guerreiro da-
dc.contributor.authorMALIQUEO, Manuel-
dc.contributor.authorBARACAT, Edmund Chada-
dc.contributor.authorMACIEL, Gustavo Arantes Rosa-
dc.date.accessioned2017-12-12T13:18:33Z-
dc.date.available2017-12-12T13:18:33Z-
dc.date.issued2017-
dc.identifier.citationCLINICS, v.72, n.8, p.510-514, 2017-
dc.identifier.issn1807-5932-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/24307-
dc.description.abstractOBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats.-
dc.description.sponsorshipFundacao de Amparo Pesquisa do Estado de Sao Paulo (FAPESP) -Brazil [2010/17417-3, 2013/12830-8]-
dc.description.sponsorshipMaster's degree scholarship Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) -Brazil [134694/2010-4]-
dc.language.isoeng-
dc.publisherHOSPITAL CLINICAS, UNIV SAO PAULO-
dc.relation.ispartofClinics-
dc.rightsopenAccess-
dc.subjectPolycystic Ovary Syndrome-
dc.subjectHypothalamus-
dc.subjectAnimal Models-
dc.subjectKisspeptin-
dc.subjectTestosterone-
dc.subject.otherhormone neurons-
dc.subject.otherfemale rats-
dc.subject.otherexposure-
dc.subject.otherdihydrotestosterone-
dc.subject.othertestosterone-
dc.subject.otherfertility-
dc.subject.otherestradiol-
dc.subject.otherfeedback-
dc.subject.otherpathway-
dc.subject.otherpulse-
dc.titleHypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes-
dc.typearticle-
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULO-
dc.identifier.doi10.6061/clinics/2017(08)09-
dc.identifier.pmid28954011-
dc.subject.wosMedicine, General & Internal-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalGIANNOCCO, Gisele:Univ Fed Sao Paulo, Dept Med, Lab Endocrinol Mol & Translac, Sao Paulo, Brazil-
hcfmusp.author.externalSILVA, Ismael Dale Cotrim Guerreiro da:Univ Fed Sao Paulo, Escola Paulista Med, Dept Ginecol, Lab Ginecol Mol & Proteom, Sao Paulo, SP, Brazil-
hcfmusp.author.externalMALIQUEO, Manuel:Univ Chile, West Div, Dept Med, Endocrinol & Metab Lab, Santiago, Chile-
hcfmusp.description.beginpage510-
hcfmusp.description.endpage514-
hcfmusp.description.issue8-
hcfmusp.description.volume72-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:000411803900009-
hcfmusp.origem.id2-s2.0-85030184659-
hcfmusp.origem.idSCIELO:S1807-59322017000800510-
hcfmusp.publisher.citySAO PAULO-
hcfmusp.publisher.countryBRAZIL-
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dc.description.indexMEDLINE-
dc.identifier.eissn1980-5322-
hcfmusp.citation.scopus5-
hcfmusp.scopus.lastupdate2022-11-04-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MOG
Departamento de Obstetrícia e Ginecologia - FM/MOG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/58
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular


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