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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorCOSTA-DA-SILVA, Andre Luis-
dc.contributor.authorNAVARRETE, Flavia Rosa-
dc.contributor.authorSALVADOR, Felipe Scassi-
dc.contributor.authorKARINA-COSTA, Maria-
dc.contributor.authorIOSHINO, Rafaella Sayuri-
dc.contributor.authorAZEVEDO, Diego Soares-
dc.contributor.authorROCHA, Desiree Rafaela-
dc.contributor.authorROMANO, Camila Malta-
dc.contributor.authorCAPURRO, Margareth Lara-
dc.identifier.citationPLOS ONE, v.8, n.1, article ID e53816, 5p, 2013-
dc.description.abstractAedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated ""Glytube"" was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti.-
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2008/10254-1, 2009/53637-0]-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq/Departamento de Ciencia e Tecnologia - DECIT [555648/2009-5]-
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES-
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia em Entomologia Molecular - INCT-EM-
dc.relation.ispartofPlos One-
dc.titleGlytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti-
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCE-
dc.subject.wosMultidisciplinary Sciences-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-, Andre Luis:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Flavia Rosa:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Felipe Scassi:Univ Sao Paulo, Virol Lab, Inst Trop Med, Sao Paulo, Brazil-, Maria:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Rafaella Sayuri:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Diego Soares:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Desiree Rafaela:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-, Margareth Lara:Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil-
hcfmusp.publisher.citySAN FRANCISCO-
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hcfmusp.remissive.sponsorshipMinistério da Saúde-
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Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

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