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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | CARVALHO, Camila Aparecida de | - |
dc.contributor.author | PARTATA, Anette Kelsei | - |
dc.contributor.author | HIRAMOTO, Roberto Mitsuyoshi | - |
dc.contributor.author | BORBOREMA, Samanta Etel Treiger | - |
dc.contributor.author | MEIRELES, Luciana Regina | - |
dc.contributor.author | NASCIMENTO, Nanci do | - |
dc.contributor.author | ANDRADE JR., Heitor Franco de | - |
dc.date.accessioned | 2013-09-23T17:00:44Z | - |
dc.date.available | 2013-09-23T17:00:44Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | ACTA TROPICA, v.125, n.2, p.128-136, 2013 | - |
dc.identifier.issn | 0001-706X | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/2466 | - |
dc.description.abstract | Visceral leishmaniasis, caused by Leishmania (Leishmania) chagasi, is a chronic parasitic disease of humans and dogs. Confirmation of the protozoal agent in bone marrow, lymph node or spleen aspirate is diagnostic, while specific-IgG serology is used mainly for epidemiology despite the general presence of high levels of serum immunoglobulin. Anecdotal reports of false-negative serology in active disease cases are known and are ascribed to the formation of immune complexes. Because dissociation of immune complexes can be accomplished by acid treatment, we devised a simple, routine enzyme immunoassay (ELISA) for the dissociation of immune complexes in serum samples using acid treatment in wells adsorbed with Leishmania antigen (dELISA). Confirmatory acid dot-blot was also developed for antigen detection by anti-Leishmania rabbit antiserum. In experimental L. chagasi hamster models, immune complexes interfered with ELISA mostly in the 30 and 60 days postinfection, according to both dELISA and antigen dot-blot results. In larger samples from endemic areas, dELISA was positive in 10% of seronegative dog samples (7/70) and 3.5% in negative human samples (3/88), showing that dELISA could be used in the serodiagnosis of visceral leishmaniasis. Moreover, dELISA could be used as an alternative approach to screening asymptomatic visceral leishmaniasis patients, instead of invasive confirmatory testing. | - |
dc.description.sponsorship | [FMUSP-HC/LIMs] | - |
dc.language.iso | eng | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.ispartof | Acta Tropica | - |
dc.rights | restrictedAccess | - |
dc.subject | Immune complexes | - |
dc.subject | ELISA | - |
dc.subject | Interference | - |
dc.subject.other | linked-immunosorbent-assay | - |
dc.subject.other | antigen-antibody dissociation | - |
dc.subject.other | human-immunodeficiency-virus | - |
dc.subject.other | visceral leishmaniasis | - |
dc.subject.other | circulating antigen | - |
dc.subject.other | p24 antigen | - |
dc.subject.other | diagnosis | - |
dc.subject.other | infection | - |
dc.subject.other | donovani | - |
dc.subject.other | serodiagnosis | - |
dc.title | A simple immune complex dissociation ELISA for leishmaniasis: Standardization of the assay in experimental models and preliminary results in canine and human samples | - |
dc.type | article | - |
dc.rights.holder | Copyright ELSEVIER SCIENCE BV | - |
dc.identifier.doi | 10.1016/j.actatropica.2012.10.010 | - |
dc.identifier.pmid | 23123344 | - |
dc.subject.wos | Parasitology | - |
dc.subject.wos | Tropical Medicine | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | CARVALHO, Camila Aparecida de:Univ Sao Paulo, Protozool Lab, IMTSP, BR-05403000 Sao Paulo, Brazil | - |
hcfmusp.author.external | PARTATA, Anette Kelsei:Inst Pesquisas Energet & Nucl, Ctr Biotechnol, BR-05508000 Sao Paulo, Brazil | - |
hcfmusp.author.external | HIRAMOTO, Roberto Mitsuyoshi:Adolfo Lutz Inst, BR-01246000 Sao Paulo, Brazil | - |
hcfmusp.author.external | BORBOREMA, Samanta Etel Treiger:Adolfo Lutz Inst, BR-01246000 Sao Paulo, Brazil | - |
hcfmusp.author.external | NASCIMENTO, Nanci do:Inst Pesquisas Energet & Nucl, Ctr Biotechnol, BR-05508000 Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 128 | - |
hcfmusp.description.endpage | 136 | - |
hcfmusp.description.issue | 2 | - |
hcfmusp.description.volume | 125 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000314016100002 | - |
hcfmusp.origem.id | 2-s2.0-84870665845 | - |
hcfmusp.publisher.city | AMSTERDAM | - |
hcfmusp.publisher.country | NETHERLANDS | - |
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dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | FMUSP-HC | - |
hcfmusp.citation.scopus | 23 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/49 |
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