Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author SESSO, Antonio FMUSP-HC
YAMASHIRO-KANASHIRO, Edite Hatsumi FMUSP-HC
ARRUDA, Lia Barbara FMUSP-HC
KAWAKAMI, Joyce FMUSP-HC
HIGUCHI, Maria de Lourdes FMUSP-HC
ORII, Noemia Mie FMUSP-HC
TANIWAKI, Noemi Nosomi
CARVALHO, Falvia Mendes da Cunha FMUSP-HC
BRITO, Mariane Pereira FMUSP-HC
GOTTARDI, Maiara
CARNEIRO, Sylvia Mendes
TAGA, Rumio
dc.date.issued 2017
dc.identifier.citation REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.59, article ID UNSP e84, 17p, 2017
dc.identifier.issn 0036-4665
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/25681
dc.description.abstract A study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after PCR and sequencing experiments. Bacteria probably originated from a cytoplasm with compacted tiny granular particles replacing the normal cytosol territories, or from the contact with the cytoplasm through a clear semi-solid material. The compact granularity (CG) of the cytoplasm was crossed by stripes of smooth and rough endoplasmic reticulum cisternae. Among apparently normal mitochondria, it was noted, in variable proportions, mitochondria with crista-delimited lucent central regions that expand to and occupied the interior of a crista-less organelle, which can undergo fission. Other components of the scenarios of mycoplasma-induced cell demolition are villus-like structures with associated 80-200 nm vesicles and a clear, flexible semi-solid, process-sensitive substance that we named jam-like material. This material coated the cytoplasmic surface, its recesses, irregular protrusions and detached cytoplasmic fragments. It also cushioned forming bacteria. Cyst-like structures were often present in the cytoplasm. Cells, mainly apoptotic, exhibiting ample cytoplasmic sectors with characteristic net-like profile due to adjoined vacuoles, as well as ovoid or elongated profiles, consistently appeared in all cells from the last four cell donors. These cells were named ""modified host cells"" because bacteria arose in the vacuoles. The possibility that, in some samples, there was infection and/or coinfection of the host cell by another organism(s) cannot be ruled out.
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/22.816-2]
dc.language.iso eng
dc.publisher INST MEDICINA TROPICAL SAO PAULO
dc.relation.ispartof Revista do Instituto de Medicina Tropical de Sao Paulo
dc.rights openAccess
dc.subject Mycoplasma; Endoplasmic reticulum; Mitochondria; Cytosol
dc.subject.other electron-microscopy; fermentans; hyorhinis; pneumoniae; genitalium; apoptosis; membranes; invasion; cultures
dc.title Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles
dc.type article
dc.rights.holder Copyright INST MEDICINA TROPICAL SAO PAULO
dc.description.group LIM/06
dc.description.group LIM/48
dc.description.group LIM/56
dc.identifier.doi 10.1590/S1678-9946201759084
dc.identifier.pmid 29267592
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author SESSO, Antonio:FM:MPT
hcfmusp.author YAMASHIRO-KANASHIRO, Edite Hatsumi:IMT:SCSORO-83
hcfmusp.author ARRUDA, Lia Barbara:FM:
hcfmusp.author KAWAKAMI, Joyce:HC:INCOR
hcfmusp.author HIGUCHI, Maria de Lourdes:HC:INCOR
hcfmusp.author ORII, Noemia Mie:FM:MDT
hcfmusp.author CARVALHO, Falvia Mendes da Cunha:FM:
hcfmusp.author BRITO, Mariane Pereira:IMT:
hcfmusp.author.external · TANIWAKI, Noemi Nosomi:Adolfo Lutz Inst, Lab Microscopia Eletron, Sao Paulo, SP, Brazil
· GOTTARDI, Maiara:Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Imunopatol, Setor Biol Estrutural, LIM 06, Sao Paulo, SP, Brazil
· CARNEIRO, Sylvia Mendes:Inst Butantan, Lab Biol Celular, Sao Paulo, SP, Brazil
· TAGA, Rumio:Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, Disciplina Histol, Sao Paulo, Brazil; Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, Disciplina Embriol, Sao Paulo, Brazil
hcfmusp.origem.id WOS:000418613300010
hcfmusp.origem.id 2-s2.0-85041750034
hcfmusp.origem.id SCIELO:S0036-46652017005000251
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
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dc.description.index MEDLINE
dc.identifier.eissn 1678-9946
hcfmusp.citation.scopus 0
hcfmusp.citation.wos 0
hcfmusp.affiliation.country Brasil


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