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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | SILVA, Pedro Henrique Ramos Quintino da | - |
dc.contributor.author | PARRA, Edwin Roger | - |
dc.contributor.author | ZOCOLARO, William Sanches | - |
dc.contributor.author | NARDE, Ivy | - |
dc.contributor.author | RODRIGUES, Fabiola | - |
dc.contributor.author | KAIRALLA, Ronaldo Adib | - |
dc.contributor.author | CARVALHO, Carlos Roberto Ribeiro | - |
dc.contributor.author | CAPELOZZI, Vera Luiza | - |
dc.date.accessioned | 2013-07-30T14:35:03Z | - |
dc.date.available | 2013-07-30T14:35:03Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | JORNAL BRASILEIRO DE PNEUMOLOGIA, v.38, n.3, p.321-330, 2012 | - |
dc.identifier.issn | 1806-3713 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/256 | - |
dc.description.abstract | Objective: To investigate the significance of cellular immune markers, as well as that of collagen and elastic components of the extracellular matrix, within granulomatous structures in biopsies of patients with pulmonary or extrapulmonary sarcoidosis. Methods: We carried out qualitative and quantitative evaluations of inflammatory cells, collagen fibers, and elastic fibers in granulomatous structures in surgical biopsies of 40 patients with pulmonary and extrapulmonary sarcoidosis using histomorphometry, immunohistochemistry, picrosirius red staining, and Weigert's resorcin-fuchsin staining. Results: The extrapulmonary tissue biopsies presented significantly higher densities of lymphocytes, macrophages, and neutrophils than did the lung tissue biopsies. Pulmonary granulomas showed a significantly higher number of collagen fibers and a lower density of elastic fibers than did extrapulmonary granulomas. The amount of macrophages in the lung samples correlated with FVC (p < 0.05), whereas the amount of CD3+, CD4+, and CD8+ lymphocytes correlated with the FEV1/FVC ratio and VC. There were inverse correlations between TLC and the CD1a+ cell count (p < 0.05), as well as between DLCO and collagen/elastic fiber density (r = -0.90; p = 0.04). Conclusions: Immunophenotyping and remodeling both showed differences between pulmonary and extrapulmonary sarcoidosis in terms of the characteristics of the biopsy samples. These differences correlated with the clinical and spirometric data obtained for the patients, suggesting that two different pathways are involved in the mechanism of antigen clearance, which was more effective in the lungs and lymph nodes. | - |
dc.description.abstract | Objetivo: Investigar o significado de marcadores de imunidade celular e de componentes elásticos/colágeno da matriz extracelular em estruturas granulomatosas em biópsias de pacientes com sarcoidose pulmonar ou extrapulmonar. Métodos: Determinações qualitativas e quantitativas de células inflamatórias, de fibras de colágeno e de fibras elásticas em estruturas granulomatosas em biópsias cirúrgicas de 40 pacientes com sarcoidose pulmonar e extrapulmonar foram realizadas por histomorfometria, imuno-histoquímica, e técnicas de coloração com picrosirius e resorcina-fucsina de Weigert. Resultados: A densidade de linfócitos, macrófagos e neutrófilos nas biópsias extrapulmonares foi significativamente maior do que nas biópsias pulmonares. Os granulomas pulmonares apresentaram uma quantidade significativamente maior de fibras de colágeno e menor densidade de fibras elásticas que os granulomas extrapulmonares. A quantidade de macrófagos nos granulomas pulmonares correlacionou-se com CVF (p < 0,05), ao passo que as quantidades de linfócitos CD3+, CD4+ e CD8+ correlacionaram-se com a relação VEF 1 /CVF e com CV. Houve correlações negativas entre CPT e contagem de células CD1a+ (p < 0,05) e entre DLCO e densidade de fibras colágenas/elásticas (r = −0,90; p = 0,04). Conclusões: A imunofenotipagem e o remodelamento apresentaram características diferentes nas biópsias dos pacientes com sarcoidose pulmonar e extrapulmonar. Essas diferenças correlacionaram-se com os dados clínicos e espirométricos dos pacientes, sugerindo que há duas vias envolvidas no mecanismo de depuração de antígenos, que foi mais eficaz nos pulmões e linfonodos. | - |
dc.description.sponsorship | Brazilian Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, National Council for Scientific and Technological Development) | - |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo Research Foundation) [2007/56617-5] | - |
dc.language.iso | eng | - |
dc.language.iso | por | - |
dc.publisher | SOC BRASILEIRA PNEUMOLOGIA TISIOLOGIA | - |
dc.relation.ispartof | Jornal Brasileiro de Pneumologia | - |
dc.rights | openAccess | - |
dc.subject | Sarcoidosis | - |
dc.subject | Granulomatous disease, chronic | - |
dc.subject | Extracellular matrix | - |
dc.subject | Immunophenotyping | - |
dc.subject | Respiratory function tests | - |
dc.subject | Sarcoidose | - |
dc.subject | Doença granulomatosa crônica | - |
dc.subject | Matriz extracelular | - |
dc.subject | Imunofenotipagem | - |
dc.subject | Testes de função respiratória | - |
dc.subject.other | idiopathic interstitial pneumonias | - |
dc.subject.other | prognostic-significance | - |
dc.subject.other | fibrosis | - |
dc.subject.other | fibers | - |
dc.subject.other | statement | - |
dc.subject.other | secondary | - |
dc.subject.other | collagen | - |
dc.subject.other | system | - |
dc.title | Immunophenotyping and extracellular matrix remodeling in pulmonary and extrapulmonary sarcoidosis | - |
dc.title.alternative | Imunofenotipagem e remodelamento da matriz extracelular na sarcoidose pulmonar e extrapulmonar | - |
dc.type | article | - |
dc.rights.holder | Copyright SOC BRASILEIRA PNEUMOLOGIA TISIOLOGIA | - |
dc.identifier.doi | 10.1590/s1806-37132012000300007 | - |
dc.identifier.pmid | 22782602 | - |
dc.subject.wos | Respiratory System | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | RODRIGUES, Fabiola:Univ Sao Paulo, Sch Med, Dept Pathol, Hosp Clin, Sao Paulo, Brazil; Univ Sao Paulo, Sch Med, Dept Resp Dis, Inst Coracao InCor,Heart Inst,Hosp Clin, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 321 | - |
hcfmusp.description.endpage | 330 | - |
hcfmusp.description.issue | 3 | - |
hcfmusp.description.volume | 38 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84864023338 | - |
hcfmusp.origem.id | WOS:000306163500007 | - |
hcfmusp.origem.id | SCIELO:S1806-37132012000300007 | - |
hcfmusp.publisher.city | BRASILIA DF | - |
hcfmusp.publisher.country | BRAZIL | - |
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dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | CNPq | - |
hcfmusp.remissive.sponsorship | FAPESP | - |
hcfmusp.lim.ref | 2012 | - |
hcfmusp.citation.scopus | 4 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/09 |
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art_SILVA_Immunophenotyping_and_extracellular_matrix_remodeling_in_pulmonary_and_2012_eng.PDF | publishedVersion (English) | 14.44 MB | Adobe PDF | View/Open |
art_SILVA_Immunophenotyping_and_extracellular_matrix_remodeling_in_pulmonary_and_2012_por.PDF | publishedVersion (Portuguese) | 1.97 MB | Adobe PDF | View/Open |
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