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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorMAGRI, Mariana Cavalheiro-
dc.contributor.authorCOSTA, Emanuela Avelar Silva-
dc.contributor.authorCATERINO-DE-ARAUJO, Adele-
dc.date.accessioned2013-10-02T19:43:23Z-
dc.date.available2013-10-02T19:43:23Z-
dc.date.issued2012-
dc.identifier.citationVIROLOGY JOURNAL, v.9, article ID 184, 3p, 2012-
dc.identifier.issn1743-422X-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/2646-
dc.description.abstractBackground: In Virology Journal 2011, 8: 535, Neto et al. described point mutations into Tax-responsive elements (TRE) of the LTR region of HTLV-1 isolates from asymptomatic carriers from Sao Paulo, Brazil, and hypothesized that the presence of the G232A mutation in the TRE-1 increase viral proliferation and consequently the proviral load (PvL), while the A184G mutation in the TRE-2 do not have such effect. Findings: We performed the real-time PCR assay (pol) and sequenced LTR region of HTLV-1 isolates from 24 HIV/HTLV-1-coinfected patients without HTLV-1-associated diseases from the same geographic area. These sequences were classified as belonging to the transcontinental subgroup A of the Cosmopolitan subtype a. The frequency of G232A mutation (16/24, 66.7%) was high as much as 61.8% reported by Neto's in HTLV-1 asymptomatic carriers with high PvL. High frequency (13/24, 54.2%) of double mutations G232A and A184G was also detected in HIV/HTLV-1-coinfected patients. We did not quantify PvL, but comparative analyses of the cycle threshold (Ct) median values of the group of isolates presenting the mutated-types sequences (Ct 33.5, n = 16) versus the group of isolates with the wild-type sequences (Ct 32, n = 8) showed no statistical difference (p = 0.4220). Conclusion: The frequencies of mutated-type sequences in the TRE-1 and TRE-2 motifs were high in HIV/HTLV-1-coinfected patients from Sao Paulo, Brazil. If these LTR point mutations have predictive value for the development of HTLV-1-associated diseases or they correspond to the subtype of virus that circulate in this geographic area has to be determined.-
dc.description.sponsorshipMinisterio da Ciencia e Tecnologia/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (MCT/CNPq), Brasil [481040/2007-2, 303328/2009-6]-
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brasil-
dc.description.sponsorshipCAPES/PROEX - Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo (USP)-
dc.description.sponsorshipInstituto Adolfo Lutz [33/07, 39/07]-
dc.language.isoeng-
dc.publisherBIOMED CENTRAL LTD-
dc.relation.ispartofVirology Journal-
dc.rightsopenAccess-
dc.subjectHTLV-1-
dc.subjectLTR region-
dc.subjectSequencing-
dc.titleLTR point mutations in the Tax-responsive elements of HTLV-1 isolates from HIV/HTLV-1-coinfected patients-
dc.typearticle-
dc.rights.holderCopyright BIOMED CENTRAL LTD-
dc.identifier.doi10.1186/1743-422X-9-184-
dc.identifier.pmid22947305-
dc.subject.wosVirology-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalCATERINO-DE-ARAUJO, Adele:Adolfo Lutz Inst, Dept Immunol, BR-01246902 Sao Paulo, Brazil; Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo, Brazil-
hcfmusp.description.articlenumber184-
hcfmusp.description.volume9-
hcfmusp.origemWOS-
hcfmusp.origem.id2-s2.0-84865654226-
hcfmusp.origem.idWOS:000310734800001-
hcfmusp.publisher.cityLONDON-
hcfmusp.publisher.countryENGLAND-
hcfmusp.relation.referenceCosta EAS, 2011, J VIROL METHODS, V173, P280, DOI 10.1016/j.jviromet.2011.02.018-
hcfmusp.relation.referenceMagri MC, 2012, AIDS RES HUM RETROV, V28, P110, DOI [10.1089/AID.2011.0117, 10.1089/aid.2011.0117]-
hcfmusp.relation.referenceMONTAGNE J, 1990, EMBO J, V9, P957-
hcfmusp.relation.referenceNeto WK, 2011, VIROL J, V8, DOI 10.1186/1743-422X-8-535-
dc.description.indexMEDLINE-
hcfmusp.remissive.sponsorshipCAPES-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.remissive.sponsorshipUSP-
hcfmusp.lim.ref2012-
hcfmusp.citation.scopus4-
hcfmusp.scopus.lastupdate2022-09-30-
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Artigos e Materiais de Revistas Científicas - LIM/47
LIM/47 - Laboratório de Hepatologia por Vírus


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