Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/2738
Title: ACTION OF HYPERTONIC SALINE SOLUTION (NaCl 7,5%) IN PULMONARY FIBROSIS IN A RODENT MODEL OF ENDOTOXEMIA
Authors: PETRONI, Ricardo CostaBISELLI, PaoloMARTINS, MiltonCSABA, SzaboSORIANO, Francisco
Citation: FASEB JOURNAL, v.26, 2012
Abstract: Hypertonic saline solution (HSS, NaCl 7,5%) has shown to modulates immune function favorably and decrease pulmonary injury triggered by endotoxic shock. Therefore, our objective was to investigate the effects of HSS on the mechanism involved in pulmonary fibrosis, in an experimental model of endotoxemic shock. Wistar rats (220–260 g) received lipopolysaccharide - LPS (10mg/kg, intraperitoneal,ip) and volume after 15 minutes. The animals were assigned in four groups (n=7 per group): control group (not subjected to LPS); LPS group (injected with LPS 10mg/kg i.p); HSS group (injected with LPS and treated with hypertonic saline, 4 mL/Kg) and NS group (injected with LPS and treated with normal saline, 34 mL/kg). At 24h after treatment, pulmonary mechanics, type I collagen expression, metalloproteinase 9 expression and activity, and focal adhesion kinase (FAK) were measured. Normal saline increased pulmonary resistance and elastance, compared to other groups (p<0.05). HSS inhibited collagen expression compared to LPS and NS groups and prevented pulmonary injury by decreased MMP9 activity in tissue (p<0.05). Expression of FAK was decreased in HSS groups compared to LPS and NS groups (P<0.05). Therefore, we concluded that treatment of endotoxemic shock with HSS solution can decreased pulmonary fibrosis and injury through FAK pathway.
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Comunicações em Eventos - FM/MCM
Departamento de Clínica Médica - FM/MCM

Comunicações em Eventos - HC/ICHC
Instituto Central - HC/ICHC

Comunicações em Eventos - LIM/20
LIM/20 - Laboratório de Terapêutica Experimental

Comunicações em Eventos - LIM/51
LIM/51 - Laboratório de Emergências Clínicas


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