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Título: Effect of the tolerance to LPS in the cytokine production and activity of epigenetic enzymes in endotoxemic mice
Autor: RIOS, Ester Correia SarmentoLIMA, Thais Martins DeSORIANO, Francisco Garcia
Citación: FASEB JOURNAL, v.27, 2013
Resumen: Epigenetic alteration could be associated to pre-conditioning procedures. Chromatin remodeling in LPS tolerance is achieved by histone acetylation. LPS low doses induce a gene hypo-transcription in acute immune response. We investigated how the tolerance to LPS could regulate the epigenetic response in the endotoxemia. Male "Balb C" mice were divided in: C group – control, not manipulated; TD group – induced tolerance and challenged -1mg/Kg LPS during 5 days before the challenge (5mg/Kg LPS); D group – challenged without tolerance - 100μL of NaCl 0.9% during 5 days before the challenge (5mg/kg LPS). Spleens and plasma were collected 48 hours after the challenge. Activity of Histone Acetyltransferases (HAT) and Histone Deacetylases (HDAC) were assessed by colorimetric kits (abcam and biovision); Acetylation of histones H3 and H4 were analyzed by western blotting; cytokines production was measured by ELISA. Results represent the media of 5 animals. Statistical analysis was performed by ANOVA. Production of IL-6, IL-10 and TNF-α was inhibited by the induction of the tolerance compared to D group. HAT and HDAC activities were increased in the TD group compared to C/D groups (p<0.05). H3 acetylated was reduced in TD compared to D. H4 acetylated was maintained at basal levels in TD and D groups. In conclusion, the reduction of cytokines during the tolerance to LPS is associated to H3 acetylation.
Aparece en las colecciones:

Comunicações em Eventos - FM/MCM
Departamento de Clínica Médica - FM/MCM

Comunicações em Eventos - HC/ICHC
Instituto Central - HC/ICHC

Comunicações em Eventos - LIM/51
LIM/51 - Laboratório de Emergências Clínicas


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