Use este identificador para citar ou linkar para este item: https://observatorio.fm.usp.br/handle/OPI/2786
Título: EFFECTS OF STORED RED BLOOD CELL TRANSFUSION IN CARDIOPULMONARY FUNCTION AND INFLAMMATION: AN EXPERIMENTAL MECHANISTIC STUDY
Autor(es): BIAGINI, S.COSTA, P. A.DALE, C. S.MONTE-REAL, E. S.MOREIRA, E. S.CARVALHO, C. R. R.SCHETTINO, G. P. P.WENDEL, S.AZEVEDO, L. C. P.
Parte de: VOX SANGUINIS, v.103, suppl.1, Special Issue, p.21-21, 2012
Resumo: Background: Red blood cell (RBC) transfusion has been associated to deleterious inflammatory and respiratory effects. However, the mechanisms related to these phenomena are incompletely understood. Aims: To develop an experimental model of homologous stored RBC transfusion using hypovolemic swine aiming to evaluate its acute effect on cardiopulmonary function, microcirculation, pulmonary and systemic inflammation. Methods: Sixteen anesthetized and instrumented swine (60–70 kg) were submitted to controlled hemorrhage (25% of volemia). Two units of non-filtered RBC from each animalwereprocessedandstoredfor14 days.Afterhypovolemia,eightanimalsreceived Lactated Ringer’s solution (three times the removed blood volume). The transfusion group received the two units of RBC and Lactated Ringer’s solution (to complete blood volume removed). Both groups were followed up to 6 h after resuscitation. Microcirculation was assessed with a laserdopplerflowmetry probe at ileum. Plasma and pulmonarycytokines’ concentrations(IL-6, IL-1,IL-10, IL-21, TNF-alpha) were measuredas well as nitrate concentrations and cytokines and NOS2 gene expression in the lungs. Results: RBC transfusion significantly increased oxygen venous saturation and oxygen delivery. Microcirculatory evaluation was similar between groups, and also were pulmonary mechanics and gas exchange. Nitrate and cytokines concentration in the plasma and lungs were similar between groups. However, RT-qPCR for cytokinesdemonstrated an increase (twofold) in mRNA level for NOS2 and a 0.5-fold decrease in mRNA content for IL-21 in the transfused group. Conclusions: Stored homologous RBC transfusion does not induce clinically significant effects on microcirculation and respiratory function up to 6 h after transfusion.
Aparece nas coleções:

Comunicações em Eventos - FM/MCP
Departamento de Cardio-Pneumologia - FM/MCP

Comunicações em Eventos - HC/ICHC
Instituto Central - HC/ICHC

Comunicações em Eventos - HC/InCor
Instituto do Coração - HC/InCor

Comunicações em Eventos - LIM/09
LIM/09 - Laboratório de Pneumologia

Comunicações em Eventos - LIM/51
LIM/51 - Laboratório de Emergências Clínicas


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