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Title: Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice
Authors: RIGHETTI, Renato FragaSANTOS, Tabata Maruyama dosCAMARGO, Leandro do NascimentoARISTOTELES, Luciana Ritha Cassia Rolim BarbosaFUKUZAKI, SilviaSOUZA, Flavia Castro Ribas deSANTANA, Fernanda Paula RonconAGRELA, Marcus Vinicius Rodrigues deCRUZ, Maysa MarianaALONSO-VALE, Maria Isabel CardosoGENARO, Isabella SantosSARAIVA-ROMANHOLO, Beatriz MangueiraLEICK, Edna AparecidaMARTINS, Milton de ArrudaPRADO, Carla MaximoTIBERIO, Iolanda de Fatima Lopes Calvo
Citation: FRONTIERS IN PHARMACOLOGY, v.9, article ID 1021, 15p, 2018
Abstract: Introduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL-17 in acute lung injury (ALI).We investigated the mechanisms involved in the effect of anti-IL17 in a model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Methods: Mice were pre-treated with anti-IL17, 1h before saline/LPS intratracheal administration alongside non-treated controls and levels of exhaled nitric oxide (eNO), cytokine expression, extracellular matrix remodeling and oxidative stress, as well as immune cell counts in bronchoalveolar lavage fluid (BALF), and respiratory mechanics were assessed in lung tissue. Results: LPS instillation led to an increase in multiple cytokines, proteases, nuclear factor-kappa B, and Forkhead box P3 (FOXP3), eNO and regulators of the actomyosin cytoskeleton, the number of CD4+ and iNOS-positive cells as well as the number of neutrophils and macrophages in BALF, resistance and elastance of the respiratory system, ARG-1 gene expression, collagen fibers, and actin and 8-iso-PGF2 alpha volume fractions. Pre-treatment with anti-IL17 led to a significant reduction in the level of all assessed factors. Conclusions: Anti-IL17 can protect the lungs from the inflammatory effects of LPS-induced ALI, primarily mediated by the reduced expression of cytokines and oxidative stress. This suggests that further studies using anti-IL17 in a treatment regime would be highly worthwhile.
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Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/20
LIM/20 - Laboratório de Terapêutica Experimental

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