Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/29429
Title: Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer
Authors: ROGERI, Caroline DominguesSILVEIRA, Henrique Cesar SantejoCAUSIN, Rhafaela LimaVILLA, Luisa LinaSTEIN, Maira DegiovaniCARVALHO, Ana Carolina deARANTES, Lidia Maria Rebolho BatistaSCAPULATEMPO-NETO, CristovamPOSSATI-RESENDE, Julio CesarANTONIAZZI, MarcioLONGATTO-FILHO, AdhemarFREGNANI, Jose Humberto Tavares Guerreiro
Citation: GYNECOLOGIC ONCOLOGY, v.150, n.3, p.545-551, 2018
Abstract: Objectives. The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Methods. A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a ON grade of 2 or 3 (CIN 2/3; n = 148). The methylation pattern for a panel of 15 genes was analysed by quantitative methylation-specific PCR (qMSP) and compared between the groups (<= CIN 1 vs. CIN 2+). Results. In the validation set, seven genes presented significantly different methylation profiles according to diagnosis, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), HIC1 (p = 0.028), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). Six genes showed a significant increase in the frequency of methylation in the presence of hr-HPV, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), hsa-miR-124-2 (p = 0.001), LMXIA (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). The methylation of the hsa-miR-124 gene showed sensitivity and specificity (86.7% and 613%, respectively) similar to that of the HPV test (913% and 50.0%, respectively). The independent factors associated with the diagnosis of CIN 2+ and the methylation of the hsa-miR-124-2 (OR = 5.1), SOX1 (OR = 2.8), TERT (OR = 2.2), and LMXIA (OR = 2.0) genes were a positive test for hr-HPV (odds ratio [OR] = 5.5). Conclusions. Hypermethylation of the hsa-miR-124-2, SOX1, TERT, and LMXIA genes may be a promising bio-marker for precursor lesions in cervical cancer regardless of the hr-HPV status.
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Artigos e Materiais de Revistas Científicas - FM/MDR
Departamento de Radiologia - FM/MDR

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/14
LIM/14 - Laboratório de Investigação em Patologia Hepática

Artigos e Materiais de Revistas Científicas - LIM/24
LIM/24 - Laboratório de Oncologia Experimental


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