Use este identificador para citar ou linkar para este item: https://observatorio.fm.usp.br/handle/OPI/29495
Título: Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells
Autor(es): BILHAR, Andreisa P. M.BORTOLINI, Maria A. T.SE, Alexandre B.FEITOSA, Suellen M.SALERNO, Gisela R. F.ZANOTELI, EdmarSIMOES, Manuel J.CASTRO, Rodrigo A.
Parte de: NEUROUROLOGY AND URODYNAMICS, v.37, n.7, p.2151-2159, 2018
Resumo: AimsTo identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, Vegf, Ngf, Ki67, Myh11, and Myh2; (2) expression of smooth and striated muscle proteins. MethodsMDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real-time RT-qPCR and immunohistochemistry were performed. ResultsMDSCs were identified only after 2h of the procedure in the urethra. Myh11 gene was overexpressed in the Trauma group in relation to Control. Ki67 gene expression was increased in the MDSC group relative to Trauma and Control. Col1a1 and Col3a1 genes expression were increased in the MDSC group relative to Control. Ngf mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control. ConclusionMDSCs migrated early to the traumatized urethra, but did not integrate into the tissue. MDSC alters the expression of genes related to cell proliferation, neural growth factor and extracellular matrix and the expression of smooth and striated muscle proteins in the traumatized rat urethra.
Aparece nas coleções:

Artigos e Materiais de Revistas Científicas - FM/MNE
Departamento de Neurologia - FM/MNE

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/15
LIM/15 - Laboratório de Investigação em Neurologia


Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
art_BILHAR_Molecular_and_immunohistochemical_analysis_of_the_urethra_of_2018.PDF
  Restricted Access
publishedVersion (English)12.68 MBAdobe PDFVisualizar/Abrir    Solicitar uma cópia

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.