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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | BILHAR, Andreisa P. M. | - |
dc.contributor.author | BORTOLINI, Maria A. T. | - |
dc.contributor.author | SE, Alexandre B. | - |
dc.contributor.author | FEITOSA, Suellen M. | - |
dc.contributor.author | SALERNO, Gisela R. F. | - |
dc.contributor.author | ZANOTELI, Edmar | - |
dc.contributor.author | SIMOES, Manuel J. | - |
dc.contributor.author | CASTRO, Rodrigo A. | - |
dc.date.accessioned | 2018-11-21T17:04:44Z | - |
dc.date.available | 2018-11-21T17:04:44Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | NEUROUROLOGY AND URODYNAMICS, v.37, n.7, p.2151-2159, 2018 | - |
dc.identifier.issn | 0733-2467 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29495 | - |
dc.description.abstract | AimsTo identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, Vegf, Ngf, Ki67, Myh11, and Myh2; (2) expression of smooth and striated muscle proteins. MethodsMDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real-time RT-qPCR and immunohistochemistry were performed. ResultsMDSCs were identified only after 2h of the procedure in the urethra. Myh11 gene was overexpressed in the Trauma group in relation to Control. Ki67 gene expression was increased in the MDSC group relative to Trauma and Control. Col1a1 and Col3a1 genes expression were increased in the MDSC group relative to Control. Ngf mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control. ConclusionMDSCs migrated early to the traumatized urethra, but did not integrate into the tissue. MDSC alters the expression of genes related to cell proliferation, neural growth factor and extracellular matrix and the expression of smooth and striated muscle proteins in the traumatized rat urethra. | - |
dc.language.iso | eng | - |
dc.publisher | WILEY | - |
dc.relation.ispartof | Neurourology and Urodynamics | - |
dc.rights | restrictedAccess | - |
dc.subject | adult stem cell | - |
dc.subject | regenerative medicine | - |
dc.subject | urethra | - |
dc.subject | urinary incontinence | - |
dc.subject.other | stress urinary-incontinence | - |
dc.subject.other | intrinsic sphincter deficiency | - |
dc.subject.other | simulated childbirth injury | - |
dc.subject.other | leak point pressure | - |
dc.subject.other | nerve growth-factor | - |
dc.subject.other | vaginal distension | - |
dc.subject.other | model | - |
dc.subject.other | injection | - |
dc.subject.other | contractility | - |
dc.subject.other | expression | - |
dc.title | Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells | - |
dc.type | article | - |
dc.rights.holder | Copyright WILEY | - |
dc.identifier.doi | 10.1002/nau.23567 | - |
dc.identifier.pmid | 30152522 | |
dc.subject.wos | Urology & Nephrology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | BILHAR, Andreisa P. M.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | BORTOLINI, Maria A. T.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | SE, Alexandre B.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | FEITOSA, Suellen M.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | SALERNO, Gisela R. F.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | SIMOES, Manuel J.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.author.external | CASTRO, Rodrigo A.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 2151 | - |
hcfmusp.description.endpage | 2159 | - |
hcfmusp.description.issue | 7 | - |
hcfmusp.description.volume | 37 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000444957900012 | - |
hcfmusp.origem.id | 2-s2.0-85053211076 | - |
hcfmusp.publisher.city | HOBOKEN | - |
hcfmusp.publisher.country | USA | - |
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dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1520-6777 | - |
hcfmusp.citation.scopus | 5 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MNE Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/15 |
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