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https://observatorio.fm.usp.br/handle/OPI/29570
Title: | Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
Authors: | SANTOS, Anderson Pacheco dos; CARVALHO, Maria Esther de; MEIRELLES, Luciana Regina; ANDRADE JUNIOR, Heitor Franco de |
Citation: | REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, v.51, n.5, p.665-669, 2018 |
Abstract: | Introduction: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. Methods: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. Results: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. Conclusions: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/49 |
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File | Description | Size | Format | |
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art_SANTOS_Effect_of_chaotropes_in_Chagas_disease_and_leishmaniasis_2018.PDF | publishedVersion (English) | 1.58 MB | Adobe PDF | View/Open |
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