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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | SANTOS, Anderson Pacheco dos | - |
dc.contributor.author | CARVALHO, Maria Esther de | - |
dc.contributor.author | MEIRELLES, Luciana Regina | - |
dc.contributor.author | ANDRADE JUNIOR, Heitor Franco de | - |
dc.date.accessioned | 2018-11-21T17:06:23Z | - |
dc.date.available | 2018-11-21T17:06:23Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, v.51, n.5, p.665-669, 2018 | - |
dc.identifier.issn | 0037-8682 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29570 | - |
dc.description.abstract | Introduction: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. Methods: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. Results: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. Conclusions: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays. | - |
dc.description.sponsorship | Laboratorios de Investigacao Medica 49 [LIM 49] | - |
dc.description.sponsorship | Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo (HCFMUSP) | - |
dc.description.sponsorship | Laboratorio de Imunoepidemiologia, Superintendencia de Controle de Endemias (SUCEN) | - |
dc.language.iso | eng | - |
dc.publisher | SOC BRASILEIRA MEDICINA TROPICAL | - |
dc.relation.ispartof | Revista da Sociedade Brasileira de Medicina Tropical | - |
dc.rights | openAccess | - |
dc.subject | Serology | - |
dc.subject | Chagas disease | - |
dc.subject | Leishmaniasis | - |
dc.subject | ELISA | - |
dc.subject | IFAT | - |
dc.subject | IgG antibody avidity | - |
dc.subject.other | trypanosoma-cruzi | - |
dc.subject.other | antibodies | - |
dc.subject.other | avidity | - |
dc.subject.other | igg | - |
dc.subject.other | infection | - |
dc.subject.other | antigens | - |
dc.subject.other | elisa | - |
dc.subject.other | spp. | - |
dc.title | Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys | - |
dc.type | article | - |
dc.rights.holder | Copyright SOC BRASILEIRA MEDICINA TROPICAL | - |
dc.identifier.doi | 10.1590/0037-8682-0391-2017 | - |
dc.identifier.pmid | 30304274 | |
dc.subject.wos | Parasitology | - |
dc.subject.wos | Tropical Medicine | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | SANTOS, Anderson Pacheco dos:Inst Butantan, Lab Virol, Secao Hepatite, Sao Paulo, SP, Brazil | - |
hcfmusp.author.external | CARVALHO, Maria Esther de:Secretaria Estado Saude Sao Paulo, Lab Imunoepidemiol Superintendencia Controle Ende, Sao Paulo, SP, Brazil | - |
hcfmusp.description.beginpage | 665 | - |
hcfmusp.description.endpage | 669 | - |
hcfmusp.description.issue | 5 | - |
hcfmusp.description.volume | 51 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000446591400016 | - |
hcfmusp.origem.id | 2-s2.0-85055148734 | - |
hcfmusp.origem.id | SCIELO:S0037-86822018000500665 | |
hcfmusp.publisher.city | BRASILIA | - |
hcfmusp.publisher.country | BRAZIL | - |
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dc.description.index | MEDLINE | - |
hcfmusp.citation.scopus | 1 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/49 |
Files in This Item:
File | Description | Size | Format | |
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art_SANTOS_Effect_of_chaotropes_in_Chagas_disease_and_leishmaniasis_2018.PDF | publishedVersion (English) | 1.58 MB | Adobe PDF | View/Open |
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