Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/29587
Title: Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice
Authors: DURIGON, Thomas StravinskasMACKENZIE, BreAnneOLIVEIRA-JUNIOR, Manoel CarneiroSANTOS-DIAS, AlanaANGELIS, Katia DeMALFITANO, ChristianoPALMA, Renata Kelly daGUERRA, Juliana MorenoDAMACENO-RODRIGUES, Nilsa ReginaCALDINI, Elia GarciaALMEIDA, Francine Maria deAQUINO-SANTOS, Helida CristinaRIGONATO-OLIVEIRA, Nicole CristineOLIVEIRA, Danielle Bruna Leal deAIMBIRE, FlavioOLIVEIRA, Ana Paula Ligeiro deOLIVEIRA, Luiz Vicente Franco deDURIGON, Edison LuizHIEMSTRA, Pieter S.VIEIRA, Rodolfo P.
Citation: JOURNAL OF INNATE IMMUNITY, v.10, n.4, p.279-290, 2018
Abstract: Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 x 10(4) colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1 beta (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-alpha (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-kappa B expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Preincubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-alpha (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/ NF-.B, and redox signaling. (C) 2018 S. Karger AG, Basel
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LIM/59 - Laboratório de Biologia Celular


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