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https://observatorio.fm.usp.br/handle/OPI/29587
Título: | Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice |
Autor(es): | DURIGON, Thomas Stravinskas; MACKENZIE, BreAnne; OLIVEIRA-JUNIOR, Manoel Carneiro; SANTOS-DIAS, Alana; ANGELIS, Katia De; MALFITANO, Christiano; PALMA, Renata Kelly da; GUERRA, Juliana Moreno; DAMACENO-RODRIGUES, Nilsa Regina; CALDINI, Elia Garcia; ALMEIDA, Francine Maria de; AQUINO-SANTOS, Helida Cristina; RIGONATO-OLIVEIRA, Nicole Cristine; OLIVEIRA, Danielle Bruna Leal de; AIMBIRE, Flavio; OLIVEIRA, Ana Paula Ligeiro de; OLIVEIRA, Luiz Vicente Franco de; DURIGON, Edison Luiz; HIEMSTRA, Pieter S.; VIEIRA, Rodolfo P. |
Parte de: | JOURNAL OF INNATE IMMUNITY, v.10, n.4, p.279-290, 2018 |
Resumo: | Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 x 10(4) colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1 beta (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-alpha (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-kappa B expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Preincubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-alpha (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/ NF-.B, and redox signaling. (C) 2018 S. Karger AG, Basel |
Aparece nas coleções: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - LIM/59 Artigos e Materiais de Revistas Científicas - ODS/03 |
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