Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/29618
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorNUNES, Juliana Barbosa-
dc.contributor.authorCOURA-VITAL, Wendel-
dc.contributor.authorCOLOMBO, Fabio Antonio-
dc.contributor.authorBAETA, Frederico Jose Moreira-
dc.contributor.authorPINHEIRO, Aimara Costa-
dc.contributor.authorROATT, Bruno Mendes-
dc.contributor.authorREIS, Levi Eduardo Soares-
dc.contributor.authorREIS, Alexandre Barbosa-
dc.contributor.authorMARQUES, Marcos Jose-
dc.date.accessioned2018-11-21T17:06:46Z-
dc.date.available2018-11-21T17:06:46Z-
dc.date.issued2018-
dc.identifier.citationPARASITOLOGY RESEARCH, v.117, n.10, p.3341-3346, 2018-
dc.identifier.issn0932-0113-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/29618-
dc.description.abstractDogs are important hosts and reservoirs of leishmaniasis, a disease caused by protozoan parasites from the genus Leishmania, affecting similar to 12 million people worldwide. The detection of visceral leishmaniasis (VL) in dogs by real-time PCR (qPCR) may improve on diagnosis, but the different qPCR methods available for Leishmania DNA detection have not been established as routine in diagnostic tools and/or epidemiologic studies for canine VL. Here, we compared three qPCR assays (DNApol, Linj31, and LDON) in the detection of VL by Leishmania infantum in spleen (n = 48; 7), skin (n = 48; 7), and whole blood (n = 44; 7) samples from serologically positive and negative dogs, respectively. Overall, the DNApol performed better than the Linj31 and LDON assays in the detection of positive samples in all tissues tested, yielding from 66.7 to 100.0% of positivity for both skin and spleen samples. For spleen samples, we observed no statistically significant differences between positive detection by the LDON and DNApol assays. Whole blood samples yielded the lowest rates of positive detection, regardless of the qPCR assay used. In contrast, positive detection of Leishmania DNA was as efficient from skin samples using the DNApol assay as from spleen samples using either the DNApol or the LDON assay. Although qPCR assays from skin samples may not be practical for use in the field, our study suggests that the DNApol and LDON assays from skin samples could be used in future to evaluate canine VL treatment in veterinary clinics.-
dc.description.sponsorshipFAPEMIG-
dc.description.sponsorshipCNPq-
dc.description.sponsorshipCAPES-
dc.description.sponsorshipFINEP-
dc.language.isoeng-
dc.publisherSPRINGER-
dc.relation.ispartofParasitology Research-
dc.rightsrestrictedAccess-
dc.subjectDogs-
dc.subjectVisceral leishmaniasis-
dc.subjectqPCR assays-
dc.subjectDiagnosis-
dc.subject.otherpolymerase-chain-reaction-
dc.subject.otherinfantum infection-
dc.subject.otherperipheral-blood-
dc.subject.otherconventional pcr-
dc.subject.otherlymph-node-
dc.subject.otherdiagnosis-
dc.subject.otherdogs-
dc.subject.othersamples-
dc.subject.otherperformance-
dc.subject.otheramplification-
dc.titleComparative analysis of real-time PCR assays in the detection of canine visceral leishmaniasis-
dc.typearticle-
dc.rights.holderCopyright SPRINGER-
dc.identifier.doi10.1007/s00436-018-6038-9-
dc.identifier.pmid30088073
dc.subject.wosParasitology-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalCOURA-VITAL, Wendel:Univ Fed Ouro Preto, Nucleus Res Biol Sci NUPEB, Ouro Preto, MG, Brazil-
hcfmusp.author.externalCOLOMBO, Fabio Antonio:Univ Fed Alfenas, Fac Pharmaceut Sci, Lab Clin Parasitol, Alfenas, MG, Brazil-
hcfmusp.author.externalBAETA, Frederico Jose Moreira:Univ Fed Alfenas UNIFAL MG, Inst Biomed Sci, Gabriel Monteiro da Silva St 700, BR-37130001 Alfenas, MG, Brazil-
hcfmusp.author.externalPINHEIRO, Aimara Costa:Municipal Hlth Secretariat, Zoonosis Control Ctr, Governador Valadares, MG, Brazil-
hcfmusp.author.externalROATT, Bruno Mendes:Univ Fed Ouro Preto, Nucleus Res Biol Sci NUPEB, Ouro Preto, MG, Brazil-
hcfmusp.author.externalREIS, Levi Eduardo Soares:Univ Fed Ouro Preto, Nucleus Res Biol Sci NUPEB, Ouro Preto, MG, Brazil-
hcfmusp.author.externalREIS, Alexandre Barbosa:Univ Fed Ouro Preto, Nucleus Res Biol Sci NUPEB, Ouro Preto, MG, Brazil-
hcfmusp.author.externalMARQUES, Marcos Jose:Univ Fed Alfenas UNIFAL MG, Inst Biomed Sci, Gabriel Monteiro da Silva St 700, BR-37130001 Alfenas, MG, Brazil-
hcfmusp.description.beginpage3341-
hcfmusp.description.endpage3346-
hcfmusp.description.issue10-
hcfmusp.description.volume117-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:000445159500035-
hcfmusp.origem.id2-s2.0-85051700950-
hcfmusp.publisher.cityNEW YORK-
hcfmusp.publisher.countryUSA-
hcfmusp.relation.referenceAntinori S, 2007, CLIN INFECT DIS, V44, P1602, DOI 10.1086/518167-
hcfmusp.relation.referenceBarrouin-Melo SM, 2004, MEM I OSWALDO CRUZ, V99, P195, DOI 10.1590/S0074-02762004000200014-
hcfmusp.relation.referenceBelinchon-Lorenzo S, 2013, VET PARASITOL, V192, P43, DOI 10.1016/j.vetpar.2012.11.007-
hcfmusp.relation.referenceBorja LS, 2016, VET PARASITOL, V229, P110, DOI 10.1016/j.vetpar.2016.10.004-
hcfmusp.relation.referenceBrasil, 2006, MAN VIG CONTR LEISHM, P120-
hcfmusp.relation.referenceBrasil, MAPA, 2016, 112016CPVDFIPSDAGMMA-
hcfmusp.relation.referenceBretagne S, 2001, CLIN DIAGN LAB IMMUN, V8, P828, DOI 10.1128/CDLI.8.4.828-831.2001-
hcfmusp.relation.referenceCavalcanti MD, 2009, VET J, V182, P356, DOI 10.1016/j.tvjl.2008.05.018-
hcfmusp.relation.referenceColombo FA, 2011, PARASITOL RES, V109, P267, DOI 10.1007/s00436-010-2247-6-
hcfmusp.relation.referenceColombo FA, 2015, EXP PARASITOL, V157, P156, DOI 10.1016/j.exppara.2015.08.014-
hcfmusp.relation.referenceCoura-Vital W, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0071833-
hcfmusp.relation.referenceCoura-Vital W, 2011, PLOS NEGLECT TROP D, V5, DOI 10.1371/journal.pntd.0001291-
hcfmusp.relation.referenceFisa R, 2001, VET PARASITOL, V99, P105, DOI 10.1016/S0304-4017(01)00447-2-
hcfmusp.relation.referenceFurrows SJ, 2001, CLIN MICROBIOL INFEC, V7, P227, DOI 10.1046/j.1469-0691.2001.00248.x-
hcfmusp.relation.referenceGlinska-Suchocka K, 2013, POL J VET SCI, V16, P835, DOI 10.2478/pjvs-2013-0118-
hcfmusp.relation.referenceGomes AHS, 2007, VET PARASITOL, V144, P234, DOI 10.1016/j.vetpar.2006.10.008-
hcfmusp.relation.referencede Amorim IFG, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0027679-
hcfmusp.relation.referenceHossein F, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0185606-
hcfmusp.relation.referenceLachaud L, 2002, PARASITOLOGY, V125, P197, DOI 10.1017/S0031182002002081-
hcfmusp.relation.referenceLachaud L, 2002, J CLIN MICROBIOL, V40, P210, DOI 10.1128/JCM.40.1.210-215.2002-
hcfmusp.relation.referenceLEVEILLE R, 1993, J AM VET MED ASSOC, V203, P413-
hcfmusp.relation.referenceManna L, 2004, VET PARASITOL, V125, P251, DOI 10.1016/j.vetpar.2004.07.019-
hcfmusp.relation.referenceMohammadiha A, 2013, VET PARASITOL, V192, P83, DOI 10.1016/j.vetpar.2012.10.013-
hcfmusp.relation.referenceMohammadiha A, 2013, EXP PARASITOL, V133, P89, DOI 10.1016/j.exppara.2012.10.017-
hcfmusp.relation.referencede Carvalho FLN, 2018, EPIDEMIOL INFECT, V146, P571, DOI 10.1017/S0950268818000225-
hcfmusp.relation.referenceNunes JB, 2016, REV INST MED TROP SP, V58, DOI [10.1590/S1678-9946201658075, 10.1590/s1678-9946201658075]-
hcfmusp.relation.referencePaz GF, 2018, ACTA TROP, V182, P198, DOI 10.1016/j.actatropica.2018.03.018-
hcfmusp.relation.referenceQuaresma PF, 2009, ACTA TROP, V111, P289, DOI 10.1016/j.actatropica.2009.05.008-
hcfmusp.relation.referenceQuinnell RJ, 2009, PARASITOLOGY, V136, P1915, DOI 10.1017/S0031182009991156-
hcfmusp.relation.referenceReimao JQ, 2011, EXP PARASITOL, V128, P111, DOI 10.1016/j.exppara.2011.02.021-
hcfmusp.relation.referenceReis AB, 2009, VET IMMUNOL IMMUNOP, V128, P87, DOI 10.1016/j.vetimm.2008.10.307-
hcfmusp.relation.referenceSambrook J, 1989, MOL CLONING-
hcfmusp.relation.referenceSchulz A, 2003, J CLIN MICROBIOL, V41, P1529, DOI 10.1128/JCM.41.4.1529-1535.2003-
hcfmusp.relation.referenceReis LES, 2013, VET PARASITOL, V197, P498, DOI 10.1016/j.vetpar.2013.07.006-
hcfmusp.relation.referenceSolca MD, 2012, VET PARASITOL, V184, P133, DOI 10.1016/j.vetpar.2011.08.026-
hcfmusp.relation.referenceVercosa BLA, 2008, BMC VET RES, V4, DOI 10.1186/1746-6148-4-45-
hcfmusp.relation.referenceVergel C, 2005, AM J TROP MED HYG, V72, P423, DOI 10.4269/ajtmh.2005.72.423-
hcfmusp.relation.referenceWerneck GL, 2016, CAD SAUDE PUBLICA, V32, DOI 10.1590/0102-311X00ED010616-
hcfmusp.relation.referenceWHO, 2010, WORKING TO OVERCOME THE GLOBAL IMPACT OF NEGLECTED TROPICAL DISEASES: FIRST WHO REPORT ON NEGLECTED TROPICAL DISEASES, P1-
dc.description.indexMEDLINE-
dc.identifier.eissn1432-1955-
hcfmusp.citation.scopus3-
hcfmusp.scopus.lastupdate2022-06-16-
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Artigos e Materiais de Revistas Científicas - LIM/50
LIM/50 - Laboratório de Patologia das Moléstias Infecciosas


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