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https://observatorio.fm.usp.br/handle/OPI/3008
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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | TEODORO, W. | - |
dc.contributor.author | CRUZ, I. Brindo da | - |
dc.contributor.author | VELOSA, A. P. | - |
dc.contributor.author | CARRASCO, S. | - |
dc.contributor.author | GOLDENSTEIN-SCHAINBERG, C. | - |
dc.contributor.author | FULLER, R. | - |
dc.contributor.author | PARRA, E. | - |
dc.contributor.author | CAPELOZZI, V. | - |
dc.date.accessioned | 2013-10-11T21:25:44Z | - |
dc.date.available | 2013-10-11T21:25:44Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | VIRCHOWS ARCHIV, v.461, suppl.1, p.S233-S233, 2012 | - |
dc.identifier.issn | 0945-6317 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/3008 | - |
dc.description.abstract | Objective: Stimulated mesenchymal stem cells (MSCs) have capacity of differentiation in many cell types. It is being used in degenerative diseases treatment protocols. We evaluated the collagen V (COL V) and collagen XI (COL XI) influence in the differentiation of rabbits adipose tissue-derived MSCs in a chondrocyte-like cell phenotype. Method: MSCs isolated of New Zealand rabbits adipose-tissue were maintained in culture by 4 weeks. COLV, COLXI and COLV/XI (10μg/ml) were added to culture during 72 h. The cells aggregates were stained with Toluidine blue, Alcian blue and Picrosirius. Chondrocyte-like phenotype was confirmed by immunofluorescence to CD34, vimentin and collagens I, II and III. Results: MSCs stimulated with COLV expressed proteoglicans and collagen, when compared with COLXI and COLV/XI and control. In the presence of COLV, MSCs was capable to increase collagen II expression confirming its chondro-cyte-like cell phenotype. In contrast, MSCs cultured with COLXI and COLV/XI express collagen I and III. Conclusion: The data suggest that COLV may facilitate the differentiation of rabbit adipose tissue-derived stem cells into a chondrocyte-like phenotype. Further studies are urged in order to evaluate the influence of COLV in the ability of chondrocytes to remodel osteoarthritic joint surface at ultrastructural and molecular levels. | - |
dc.language.iso | eng | - |
dc.publisher | SPRINGER | - |
dc.relation.ispartof | Virchows Archiv | - |
dc.rights | restrictedAccess | - |
dc.title | Collagen V induces differentiation of rabbit adipose tissue-derived stem cells in chondrocyte-like phenotype | - |
dc.type | conferenceObject | - |
dc.rights.holder | Copyright SPRINGER | - |
dc.description.conferencedate | SEP 08-12, 2012 | - |
dc.description.conferencelocal | Prague, CZECH REPUBLIC | - |
dc.description.conferencename | 24th European Congress of Pathology | - |
dc.subject.wos | Pathology | - |
dc.type.category | meeting abstract | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.beginpage | S233 | - |
hcfmusp.description.endpage | S233 | - |
hcfmusp.description.issue | suppl 1 | - |
hcfmusp.description.volume | 461 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000310364801665 | - |
hcfmusp.publisher.city | NEW YORK | - |
hcfmusp.publisher.country | USA | - |
dc.description.index | MEDLINE | - |
Appears in Collections: | Comunicações em Eventos - FM/MCM Comunicações em Eventos - FM/MPT Comunicações em Eventos - HC/ICHC Comunicações em Eventos - LIM/17 |
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