Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorTEODORO, W.-
dc.contributor.authorCRUZ, I. Brindo da-
dc.contributor.authorVELOSA, A. P.-
dc.contributor.authorCARRASCO, S.-
dc.contributor.authorGOLDENSTEIN-SCHAINBERG, C.-
dc.contributor.authorFULLER, R.-
dc.contributor.authorPARRA, E.-
dc.contributor.authorCAPELOZZI, V.-
dc.identifier.citationVIRCHOWS ARCHIV, v.461, suppl.1, p.S233-S233, 2012-
dc.description.abstractObjective: Stimulated mesenchymal stem cells (MSCs) have capacity of differentiation in many cell types. It is being used in degenerative diseases treatment protocols. We evaluated the collagen V (COL V) and collagen XI (COL XI) influence in the differentiation of rabbits adipose tissue-derived MSCs in a chondrocyte-like cell phenotype. Method: MSCs isolated of New Zealand rabbits adipose-tissue were maintained in culture by 4 weeks. COLV, COLXI and COLV/XI (10μg/ml) were added to culture during 72 h. The cells aggregates were stained with Toluidine blue, Alcian blue and Picrosirius. Chondrocyte-like phenotype was confirmed by immunofluorescence to CD34, vimentin and collagens I, II and III. Results: MSCs stimulated with COLV expressed proteoglicans and collagen, when compared with COLXI and COLV/XI and control. In the presence of COLV, MSCs was capable to increase collagen II expression confirming its chondro-cyte-like cell phenotype. In contrast, MSCs cultured with COLXI and COLV/XI express collagen I and III. Conclusion: The data suggest that COLV may facilitate the differentiation of rabbit adipose tissue-derived stem cells into a chondrocyte-like phenotype. Further studies are urged in order to evaluate the influence of COLV in the ability of chondrocytes to remodel osteoarthritic joint surface at ultrastructural and molecular levels.-
dc.relation.ispartofVirchows Archiv-
dc.titleCollagen V induces differentiation of rabbit adipose tissue-derived stem cells in chondrocyte-like phenotype-
dc.rights.holderCopyright SPRINGER-
dc.description.conferencedateSEP 08-12, 2012-
dc.description.conferencelocalPrague, CZECH REPUBLIC-
dc.description.conferencename24th European Congress of Pathology-
dc.type.categorymeeting abstract-
hcfmusp.description.issuesuppl 1-
hcfmusp.publisher.cityNEW YORK-
Appears in Collections:

Comunicações em Eventos - FM/MCM
Departamento de Clínica Médica - FM/MCM

Comunicações em Eventos - FM/MPT
Departamento de Patologia - FM/MPT

Comunicações em Eventos - HC/ICHC
Instituto Central - HC/ICHC

Comunicações em Eventos - LIM/17
LIM/17 - Laboratório de Investigação em Reumatologia

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.