Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/30126
Full metadata record
DC FieldValueLanguage
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMEDEIROS, Carla S.
dc.contributor.authorMARINO, Gustavo K.
dc.contributor.authorLASSANCE, Luciana
dc.contributor.authorSHANMUGAPRIYA, Thangavadivel
dc.contributor.authorSANTHIAGO, Marcony R.
dc.contributor.authorWILSON, Steven E.
dc.date.accessioned2019-01-17T13:37:17Z
dc.date.available2019-01-17T13:37:17Z
dc.date.issued2018
dc.identifier.citationJOURNAL OF REFRACTIVE SURGERY, v.34, n.12, p.790-798, 2018
dc.identifier.issn1081-597X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/30126
dc.description.abstractPURPOSE: To determine how photorefractive keratectomy (PRK) and mitomycin C (MMC) affect corneal nerves and their regeneration over time after surgery. METHODS: Twenty-eight New Zealand rabbits had corneal epithelial scraping with (n = 3) and without (n = 3) MMC 0.02% or -9.00 diopter PRK with (n = 6) and without (n = 16) MMC 0.02%. Corneas were removed after death and corneal nerve morphology was evaluated using acetylcholinesterase immunohistochemistry and beta-III tubulin staining after 1 day for all groups, after 1 month for PRK with and without MMC, and 2, 3, and 6 months after PRK without MMC. Image-Pro software (Media Cybernetics, Rockville, MD) was used to quantitate the area of nerve loss after the procedures and, consequently, regeneration of the nerves over time. Opposite eyes were used as controls. RESULTS: Epithelial scraping with MMC treatment did not show a statistically significant difference in nerve loss compared to epithelial scraping without MMC (P = .40). PRK with MMC was significantly different from PRK without MMC at 1 day after surgery (P = .0009) but not different at 1 month after surgery (P = .90). In the PRK without MMC group, nerves regenerated at 2 months (P < .0001) but did not return to the normal preoperative level of innervation until 3 months after surgery (P = .05). However, the morphology of the regenerating nerves was abnormal-with more tortuosity and aberrant innervation compared to the preoperative controls-even at 6 months after surgery. CONCLUSIONS: PRK negatively impacts the corneal nerves, but they are partially regenerated by 3 months after surgery in rabbits. Nerve loss after PRK extended peripherally to the excimer laser ablated zone, indicating that there was retrograde degeneration of nerves after PRK. MMC had a small additive toxic effect on the corneal nerves when combined with PRK that was only significant prior to 1 month after surgery.eng
dc.description.sponsorshipU.S. Public Health Service from the National Eye Institute, National Institutes of Health, Bethesda, MD [RO1EY10056, P30-EY025585]
dc.description.sponsorshipResearch to Prevent Blindness, Inc., New York, NY
dc.description.sponsorshipNEI [T32 EY007157]
dc.description.sponsorshipCAPES training grant PDSE2016 Brasilia, Brazil
dc.description.sponsorshipOffice of the Director, National Institutes of Health [1S10OD019972-01]
dc.language.isoeng
dc.publisherSLACK INCeng
dc.relation.ispartofJournal of Refractive Surgery
dc.rightsrestrictedAccesseng
dc.subject.othervivo confocal microscopyeng
dc.subject.otherretrograde degenerationeng
dc.subject.otherprkeng
dc.subject.otherpathophysiologyeng
dc.subject.otherinnervationeng
dc.subject.otherexpressioneng
dc.subject.othermorphologyeng
dc.subject.othersensationeng
dc.subject.otherrecoveryeng
dc.subject.otherdensityeng
dc.titleThe Impact of Photorefractive Keratectomy and Mitomycin C on Corneal Nerves and Their Regenerationeng
dc.typearticleeng
dc.rights.holderCopyright SLACK INCeng
dc.identifier.doi10.3928/1081597X-20181112-01
dc.identifier.pmid30540361
dc.subject.wosOphthalmologyeng
dc.subject.wosSurgeryeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalLASSANCE, Luciana:Cleveland Clin, Cole Eye Inst, I-32,9500 Euclid Ave, Cleveland, OH 44195 USA
hcfmusp.author.externalSHANMUGAPRIYA, Thangavadivel:Cleveland Clin, Cole Eye Inst, I-32,9500 Euclid Ave, Cleveland, OH 44195 USA
hcfmusp.author.externalWILSON, Steven E.:Cleveland Clin, Cole Eye Inst, I-32,9500 Euclid Ave, Cleveland, OH 44195 USA
hcfmusp.description.beginpage790
hcfmusp.description.endpage798
hcfmusp.description.issue12
hcfmusp.description.volume34
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000453566800001
hcfmusp.origem.id2-s2.0-85058610687
hcfmusp.publisher.cityTHOROFAREeng
hcfmusp.publisher.countryUSAeng
hcfmusp.relation.referenceAl-Louzi O, 2017, MULT SCLER J, V23, P1035, DOI 10.1177/1352458516679035eng
hcfmusp.relation.referenceAlio JL, 2013, SURV OPHTHALMOL, V58, P11, DOI 10.1016/j.survophthal.2012.04.005eng
hcfmusp.relation.referenceAmbrosio R, 2008, J REFRACT SURG, V24, P396eng
hcfmusp.relation.referenceBelmonte C, 2004, EXP EYE RES, V78, P513, DOI 10.1016/j.exer.2003.09.023eng
hcfmusp.relation.referenceBEUERMAN RW, 1980, EXP NEUROL, V69, P196, DOI 10.1016/0014-4886(80)90154-5eng
hcfmusp.relation.referenceCatapano J, 2017, PLAST RECONSTR SURG, V139, p1105E, DOI 10.1097/PRS.0000000000003257eng
hcfmusp.relation.referencede Felipe C, 1999, EUR J NEUROSCI, V11, P899, DOI 10.1046/j.1460-9568.1999.00498.xeng
hcfmusp.relation.referenceErie JC, 2005, AM J OPHTHALMOL, V140, P1059, DOI 10.1016/j.ajo.2005.07.027eng
hcfmusp.relation.referenceErie JC, 2003, CORNEA, V22, P684, DOI 10.1097/00003226-200310000-00014eng
hcfmusp.relation.referenceFrueh BE, 1998, ARCH OPHTHALMOL-CHIC, V116, P1425, DOI 10.1001/archopht.116.11.1425eng
hcfmusp.relation.referenceGambato C, 2011, CORNEA, V30, P641, DOI 10.1097/ICO.0b013e31820123c8eng
hcfmusp.relation.referenceHandley SE, 2017, NEURO-OPHTHALMOLOGY, V41, P103, DOI 10.1080/01658107.2016.1276935eng
hcfmusp.relation.referenceHe JC, 2010, EXP EYE RES, V91, P513, DOI 10.1016/j.exer.2010.07.007eng
hcfmusp.relation.referenceHeinz P, 1997, GER J OPHTHALMOL, V5, P373eng
hcfmusp.relation.referenceKauffmann T, 1997, GER J OPHTHALMOL, V5, P508eng
hcfmusp.relation.referenceKOELLE GB, 1950, AM J OPHTHALMOL, V33, P253, DOI 10.1016/0002-9394(50)90845-2eng
hcfmusp.relation.referenceKOHLHAAS M, 1992, KLIN MONATSBL AUGENH, V201, P221, DOI 10.1055/s-2008-1045898eng
hcfmusp.relation.referenceKornek GV, 1998, BRIT J CANCER, V78, P673, DOI 10.1038/bjc.1998.558eng
hcfmusp.relation.referenceLinna T, 1997, CURR EYE RES, V16, P640, DOI 10.1076/ceyr.16.7.640.5058eng
hcfmusp.relation.referenceMarfurt CF, 2010, EXP EYE RES, V90, P478, DOI 10.1016/j.exer.2009.12.010eng
hcfmusp.relation.referenceMietz H, 1997, BRIT J OPHTHALMOL, V81, P584, DOI 10.1136/bjo.81.7.584eng
hcfmusp.relation.referenceMIETZ H, 1995, INT OPHTHALMOL, V19, P89, DOI 10.1007/BF00133178eng
hcfmusp.relation.referenceMoilanen JAO, 2003, INVEST OPHTH VIS SCI, V44, P1064, DOI 10.1167/iovs.02-0247eng
hcfmusp.relation.referenceMoller-Pedersen T, 2000, OPHTHALMOLOGY, V107, P1235, DOI 10.1016/S0161-6420(00)00142-1eng
hcfmusp.relation.referenceMoody MW, 2006, OTOL NEUROTOL, V27, P1186, DOI 10.1097/01.mao.0000226306.43951.c8eng
hcfmusp.relation.referenceMOSKOWITZ PF, 1993, J NEUROSCI RES, V34, P129, DOI 10.1002/jnr.490340113eng
hcfmusp.relation.referenceMuller LJ, 2003, EXP EYE RES, V76, P521, DOI 10.1016/S0014-4835(03)00050-2eng
hcfmusp.relation.referenceNeira-Zalentein W, 2008, J REFRACT SURG, V24, P710eng
hcfmusp.relation.referenceNejima R, 2005, AM J OPHTHALMOL, V139, P64, DOI 10.1016/j.ajo.2004.08.039eng
hcfmusp.relation.referenceROZSA AJ, 1983, INVEST OPHTH VIS SCI, V24, P1033eng
hcfmusp.relation.referenceROZSA AJ, 1982, PAIN, V14, P105, DOI 10.1016/0304-3959(82)90092-6eng
hcfmusp.relation.referenceSanthiago MR, 2012, CORNEA, V31, P311, DOI 10.1097/ICO.0b013e31821e429deng
hcfmusp.relation.referenceShaheen BS, 2014, SURV OPHTHALMOL, V59, P263, DOI 10.1016/j.survophthal.2013.09.002eng
hcfmusp.relation.referenceSui T, 2014, NEURAL REGEN RES, V9, P821, DOI 10.4103/1673-5374.131598eng
hcfmusp.relation.referenceTERVO K, 1994, ARCH OPHTHALMOL-CHIC, V112, P1466, DOI 10.1001/archopht.1994.01090230080025eng
hcfmusp.relation.referenceTervo T, 2003, PROG RETIN EYE RES, V22, P339, DOI 10.1016/S1350-9462(02)00064-2eng
hcfmusp.relation.referenceTERVO T, 1976, HISTOCHEMISTRY, V47, P133, DOI 10.1007/BF00492561eng
hcfmusp.relation.referenceUchihara T, 2017, NEUROPATHOLOGY, V37, P129, DOI 10.1111/neup.12348eng
hcfmusp.relation.referenceWilson SE, 2001, OPHTHALMOLOGY, V108, P1082, DOI 10.1016/S0161-6420(01)00587-5eng
hcfmusp.relation.referenceWilson SE, 2018, J REFRACT SURG, V34, P59, DOI 10.3928/1081597X-20171128-01eng
hcfmusp.relation.referenceXia Y, 2011, EXP EYE RES, V93, P541, DOI 10.1016/j.exer.2011.06.021eng
hcfmusp.relation.referenceZANDER E, 1951, J ANAT, V85, P68eng
dc.description.indexMEDLINEeng
dc.identifier.eissn1938-2391
hcfmusp.citation.scopus11-
hcfmusp.scopus.lastupdate2022-05-06-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/Outros
Outros departamentos - FM/Outros

Artigos e Materiais de Revistas Científicas - LIM/33
LIM/33 - Laboratório de Oftalmologia


Files in This Item:
File Description SizeFormat 
art_MEDEIROS_The_Impact_of_Photorefractive_Keratectomy_and_Mitomycin_C_2018.PDF
  Restricted Access
publishedVersion (English)11.29 MBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.