Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/30305
Title: Gene expression profile of cytokines produced in biopsies from patients with American cutaneous leishmaniasis
Authors: HIPPOLITO, Daise Damaris Carnietto deGOMES, Aparecida Helena SouzaMAIA, Marta MarquesMEIRA-STREJEVITCH, Cristina da SilvaKANAMURA, Cristina TakamiLINDOSO, Jose Angelo LaulettaPEREIRA-CHIOCCOLA, Vera Lucia
Citation: ACTA TROPICA, v.189, p.69-75, 2019
Abstract: American cutaneous leishmaniasis (ACL) causes a local inflammatory process, inducing expression of several cytokine genes. Particularly, IFN-gamma can predict to disease susceptibility. Based in these data, this study was aimed to investigate the gene expression profile of IFN-gamma, IL-10, IL-27, TNF-gamma, TGF-beta and IL-6 produced in biopsies from ACL patients; and whether the gene expression profile of IFN-gamma could determine the disease evolution. Gene expression of 6 cytokines was investigated in 40 formalin-fixed paraffin embedded (FFPE) biopsies from patients with cutaneous leishmaniosis (CL); and 10 FFPE biopsies from patients with mucosal leishmaniasis (ML) (control). All 50 patients were infected with Leishmania (Viannia) braziliensis. Gene expression was determined by qPCR; and a normal control group was used for calculations (5 normal biopsies). Values were expressed as Relative Quantification (RQ). The 40 CL patients were classified into 2 groups. CLlowIFN-gamma, 35 patients with RQ for IFN-gamma below 100; and CLhighIFN-gamma, 5 (12.5%) patients with RQ above 100. Significant increase of mRNA levels of IFN-gamma, IL-10 and IL-27 was shown in CLhighIFN-gamma group when compared with CLlowIFN-gamma and ML groups. TNF-alpha levels in CLlowIFN-gamma group were higher than CLhighIFN-gamma and ML groups. TGF-beta and IL-6 were similar in 3 groups. Comparison of cytokine expression/group showed that CLlowIFN-gamma group had an equilibrium between the cytokines analyzed. In ML group, IFN-gamma was over-expressed; but in CLhighIFN-gamma group, besides IFN-gamma, IL-27 was also over-expressed. The immune response to Leishmania induces to identification of some markers, which can be determined by analysis by gene expression of cytokines produced in biopsies.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/38
LIM/38 - Laboratório de Epidemiologia e Imunobiologia


Files in This Item:
File Description SizeFormat 
art_HIPPOLITO_Gene_expression_profile_of_cytokines_produced_in_biopsies_2019.PDF
  Restricted Access
publishedVersion (English)441.8 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.