Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/30887
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorDE, De Godoy Natalia Souza
dc.contributor.authorDEMARCHI, Aiello Vera
dc.contributor.authorMAIA, De Souza Regina
dc.contributor.authorTHELMA, Okay
dc.contributor.authorALMEIDA, Braz Lucia Maria
dc.date.accessioned2019-02-21T17:26:58Z-
dc.date.available2019-02-21T17:26:58Z-
dc.date.issued2018
dc.identifier.citationIRANIAN JOURNAL OF PARASITOLOGY, v.13, n.4, p.655-660, 2018
dc.identifier.issn1735-7020
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/30887-
dc.description.abstractPatients coinfected with Leishmania/HIV can develop atypical forms of visceral leishmaniasis (VL), making it indispensable to identify the etiological agent. We are presenting a postmortemspecie definition by ITS1-PCR-RFLP in a larynx tissue of a patient presented coinfection Leishmania/HIV. This patient was from a leishmaniasis endemic region in Sao Paulo(SP), Brazil, and was diagnosed clinically with mucocutaneous leishmaniasis. Before a rK39 immunochromatographic test positive, a tiny stored paraffin-embedded larynx tissue wasobtained post-mortem and submitted to 3 conventional PCR assays: kDNA (K20/K22 and RV1/RV2), and ITS1 (LITSR/L5.8S). The last one was followed by RFLP (HaeIII) and analyzed by 4% Metaphor agarose gel electrophoresis. Leishmania genus and Leishmania (Leishmania) subgenus were defined by kDNA-PCR, with K20/K22 (120 bp) and RV1/RV2 (145 bp), respectively. ITS1-PCR-RFLP identified L. (L.) infantum chagasi species visualized by the restriction patterns of 180, 70 and 50 bp. This case draws attention to the necessity for a clear identification of the etiological agent causing infection, especially in endemicregions of cutaneous and visceral leishmaniasis, and particularly in patients with comorbidities who often present atypical forms of the disease. L. (L.) infantum chagasi, which is usually responsible for VL, had changed its clinical spectrum for mucocutaneous. Unequivocal identification was carried out by ITS-PCR-RFLP, therefore confirming rK39 result. These techniques, which complemented each other, have a convenient cost-benefit ratio that makes them suitable to be applied in developing countries.eng
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa no Estado de Sao Paulo) [2010/50304-8]
dc.language.isoeng
dc.publisherIRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGYeng
dc.relation.ispartofIranian Journal of Parasitology
dc.rightsopenAccesseng
dc.subjectVisceral leishmaniasiseng
dc.subjectHIV coinfeccioneng
dc.subjectMucocutaneous manifestationeng
dc.subjectLeishmania (L.) infantum chagasieng
dc.subjectRFLPeng
dc.subject.othervisceral leishmaniasiseng
dc.subject.otherpositive patientseng
dc.subject.otherdiagnosiseng
dc.subject.otherlesionseng
dc.titleUnusual Clinical Manifestations of Leishmania (L.) infantum chagasi in an HIV-coinfected Patient and the Relevance of ITS1-PCR-RFLP: A Case Reporteng
dc.typearticleeng
dc.rights.holderCopyright IRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGYeng
dc.identifier.pmid30697322
dc.subject.wosParasitologyeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.description.beginpage655
hcfmusp.description.endpage660
hcfmusp.description.issue4
hcfmusp.description.volume13
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000454324800019
hcfmusp.origem.id2-s2.0-85058998138
hcfmusp.publisher.cityTEHRANeng
hcfmusp.publisher.countryIRANeng
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dc.description.indexPubMedeng
dc.identifier.eissn2008-238X
hcfmusp.citation.scopus3-
hcfmusp.scopus.lastupdate2024-02-23-
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