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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | GONCALVES, Janaina G. | - |
dc.contributor.author | CANALE, Daniele | - |
dc.contributor.author | BRAGANCA, Ana Carolina de | - |
dc.contributor.author | SHIMIZU, Maria Heloisa M. | - |
dc.contributor.author | MOYSES, Rosa Maria A. | - |
dc.contributor.author | ANDRADE, Lucia | - |
dc.contributor.author | SEGURO, Antonio C. | - |
dc.contributor.author | VOLPINI, Rildo A. | - |
dc.date.accessioned | 2013-10-11T21:30:47Z | - |
dc.date.available | 2013-10-11T21:30:47Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | NEPHROLOGY DIALYSIS TRANSPLANTATION, v.28, suppl.1, p.186-186, 2013 | - |
dc.identifier.issn | 0931-0509 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/3098 | - |
dc.description.abstract | Introduction and Aims:Vitamin D deficiency (VDD) is highly prevalent in chronic kidney disease (CKD) patients. Ischemia/reperfusion-Acute Kidney Injury (IR-AKI) is considered a risk factor for CKD progression. Previous studies suggests that activation of the renin-angiotensin-aldosterone system (RAAS) and VDD could be involved in reduction of Klotho expression, an early marker of stage 1 CKD. The aim of this study was to investigate the effect of VDD on klotho expression and fibrosis development in a model of IR-AKI. Methods:Male Wistar rats (180-200g) were randomly divided into four groups (n=8 each): control (C) and ischemic (IR) fed a standard diet; VDD and VDD+IR, fed a free-vitamin D diet. On day 28, IR and VDD+IR rats were submitted to 45-minute clamping of both renal arteries. On day 90, we measured inulin clearance (GFR); Mean arterial blood pressure (MAP), renal blood flow (RBF) and calculated renal vascular resistance (RVR). We also measured serum levels of 25-hydroxyvitamin D (25(OH)D, ng/mL) and proteinuria. In renal tissue, we immunoblotted for klotho and performed imunohistochemical assay for collagen IV and fibronectin. We estimated fibrosis by fractional interstitial area (FIA) and fibronectin/collagen IV expression by score ranging from 0 to 4 according to the extension of staining. Data are expressed as mean ± SEM. Table 1. Hemodynamic and biochemical data and fractional interstitial area, Western Blot and Immunohistochemical studies. Results:Vitamin D levels were similar in C (15.4±1) and IR (15±0.6) groups and <1.5 ng/mL in VDD groups. GFR, RBF and RVR were not different among the studied groups. MAP was markedly elevated in VDD, IR and VDD+IR groups, reflecting a possible interaction on RAAS. Also, VDD, IR and VDD+IR groups showed larger areas of fibrosis and higher scores for fibronectin and collagen IV and decreased levels of klotho. Conclusions:The increased proteinuria/fibrosis and fibronectin/collagen IV expression in VDD+IR rats suggests progressive renal injury. Downregulation of klotho expression is a possible marker of chronification. Our study shows a very plausible role of VDD in this pathway as a potential stimulus for fibrosis. | - |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.language.iso | eng | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.relation.ispartof | Nephrology Dialysis Transplantation | - |
dc.rights | restrictedAccess | - |
dc.title | VITAMIN D DEFICIENCY IS ASSOCIATED WITH DOWNREGULATION OF KLOTHO AND DEVELOPMENT OF FIBROSIS IN A MURINE MODEL OF RENAL ISCHEMIA/REPERFUSION INJURY | - |
dc.type | conferenceObject | - |
dc.rights.holder | Copyright OXFORD UNIV PRESS | - |
dc.description.conferencedate | MAY 18-21, 2013 | - |
dc.description.conferencelocal | Istanbul, TURKEY | - |
dc.description.conferencename | 50th European-Renal-Association - European-Dialysis-and-Transplant-Association Congress | - |
dc.subject.wos | Transplantation | - |
dc.subject.wos | Urology & Nephrology | - |
dc.type.category | meeting abstract | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.beginpage | 186 | - |
hcfmusp.description.endpage | 186 | - |
hcfmusp.description.issue | suppl 1 | - |
hcfmusp.description.volume | 28 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000319498201062 | - |
hcfmusp.publisher.city | OXFORD | - |
hcfmusp.publisher.country | ENGLAND | - |
dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | CNPq | - |
hcfmusp.remissive.sponsorship | FAPESP | - |
Appears in Collections: | Comunicações em Eventos - FM/MCM Comunicações em Eventos - HC/ICHC Comunicações em Eventos - LIM/12 Comunicações em Eventos - LIM/16 |
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