Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/3098
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorGONCALVES, Janaina G.-
dc.contributor.authorCANALE, Daniele-
dc.contributor.authorBRAGANCA, Ana Carolina de-
dc.contributor.authorSHIMIZU, Maria Heloisa M.-
dc.contributor.authorMOYSES, Rosa Maria A.-
dc.contributor.authorANDRADE, Lucia-
dc.contributor.authorSEGURO, Antonio C.-
dc.contributor.authorVOLPINI, Rildo A.-
dc.date.accessioned2013-10-11T21:30:47Z-
dc.date.available2013-10-11T21:30:47Z-
dc.date.issued2013-
dc.identifier.citationNEPHROLOGY DIALYSIS TRANSPLANTATION, v.28, suppl.1, p.186-186, 2013-
dc.identifier.issn0931-0509-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/3098-
dc.description.abstractIntroduction and Aims:Vitamin D deficiency (VDD) is highly prevalent in chronic kidney disease (CKD) patients. Ischemia/reperfusion-Acute Kidney Injury (IR-AKI) is considered a risk factor for CKD progression. Previous studies suggests that activation of the renin-angiotensin-aldosterone system (RAAS) and VDD could be involved in reduction of Klotho expression, an early marker of stage 1 CKD. The aim of this study was to investigate the effect of VDD on klotho expression and fibrosis development in a model of IR-AKI. Methods:Male Wistar rats (180-200g) were randomly divided into four groups (n=8 each): control (C) and ischemic (IR) fed a standard diet; VDD and VDD+IR, fed a free-vitamin D diet. On day 28, IR and VDD+IR rats were submitted to 45-minute clamping of both renal arteries. On day 90, we measured inulin clearance (GFR); Mean arterial blood pressure (MAP), renal blood flow (RBF) and calculated renal vascular resistance (RVR). We also measured serum levels of 25-hydroxyvitamin D (25(OH)D, ng/mL) and proteinuria. In renal tissue, we immunoblotted for klotho and performed imunohistochemical assay for collagen IV and fibronectin. We estimated fibrosis by fractional interstitial area (FIA) and fibronectin/collagen IV expression by score ranging from 0 to 4 according to the extension of staining. Data are expressed as mean ± SEM. Table 1. Hemodynamic and biochemical data and fractional interstitial area, Western Blot and Immunohistochemical studies. Results:Vitamin D levels were similar in C (15.4±1) and IR (15±0.6) groups and <1.5 ng/mL in VDD groups. GFR, RBF and RVR were not different among the studied groups. MAP was markedly elevated in VDD, IR and VDD+IR groups, reflecting a possible interaction on RAAS. Also, VDD, IR and VDD+IR groups showed larger areas of fibrosis and higher scores for fibronectin and collagen IV and decreased levels of klotho. Conclusions:The increased proteinuria/fibrosis and fibronectin/collagen IV expression in VDD+IR rats suggests progressive renal injury. Downregulation of klotho expression is a possible marker of chronification. Our study shows a very plausible role of VDD in this pathway as a potential stimulus for fibrosis.-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.language.isoeng-
dc.publisherOXFORD UNIV PRESS-
dc.relation.ispartofNephrology Dialysis Transplantation-
dc.rightsrestrictedAccess-
dc.titleVITAMIN D DEFICIENCY IS ASSOCIATED WITH DOWNREGULATION OF KLOTHO AND DEVELOPMENT OF FIBROSIS IN A MURINE MODEL OF RENAL ISCHEMIA/REPERFUSION INJURY-
dc.typeconferenceObject-
dc.rights.holderCopyright OXFORD UNIV PRESS-
dc.description.conferencedateMAY 18-21, 2013-
dc.description.conferencelocalIstanbul, TURKEY-
dc.description.conferencename50th European-Renal-Association - European-Dialysis-and-Transplant-Association Congress-
dc.subject.wosTransplantation-
dc.subject.wosUrology & Nephrology-
dc.type.categorymeeting abstract-
dc.type.versionpublishedVersion-
hcfmusp.description.beginpage186-
hcfmusp.description.endpage186-
hcfmusp.description.issuesuppl 1-
hcfmusp.description.volume28-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:000319498201062-
hcfmusp.publisher.cityOXFORD-
hcfmusp.publisher.countryENGLAND-
dc.description.indexMEDLINE-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.remissive.sponsorshipFAPESP-
Appears in Collections:

Comunicações em Eventos - FM/MCM
Departamento de Clínica Médica - FM/MCM

Comunicações em Eventos - HC/ICHC
Instituto Central - HC/ICHC

Comunicações em Eventos - LIM/12
LIM/12 - Laboratório de Pesquisa Básica em Doenças Renais

Comunicações em Eventos - LIM/16
LIM/16 - Laboratório de Fisiopatologia Renal


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