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https://observatorio.fm.usp.br/handle/OPI/31761
Título: | Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome |
Autor(es): | SIMOES, Ricardo Santos; CARBONEL, Adriana Aparecida Ferraz; BORGES, Fernanda Teixeira; BARACAT, Maria Candida Pinheiro; SASSO, Gisela Rodrigues da Silva; SIMOES, Manuel Jesus; SERAFINI, Paulo Cesar; SOARES, Jose Maria; NADER, Helena Bonciani; BARACAT, Edmund Chada |
Parte de: | GYNECOLOGICAL ENDOCRINOLOGY, v.35, n.2, p.133-137, 2019 |
Resumo: | Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes. The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women. A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP). Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like). Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting. Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test. Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase. We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase. |
Aparece nas coleções: | Artigos e Materiais de Revistas Científicas - FM/MOG Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HU Artigos e Materiais de Revistas Científicas - LIM/58 |
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art_SIMOES_Analysis_of_hyaluronic_acid_in_the_endometrium_of_2019.PDF Restricted Access | publishedVersion (English) | 2.43 MB | Adobe PDF | Visualizar/Abrir Solicitar uma cópia |
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