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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorVIEIRA, Igor de Luna
dc.contributor.authorTAMURA, Rodrigo Esaki
dc.contributor.authorHUNGER, Aline
dc.contributor.authorSTRAUSS, Bryan E.
dc.identifier.citationJOURNAL OF INTERFERON AND CYTOKINE RESEARCH, v.39, n.4, p.246-258, 2019
dc.description.abstractTumor vasculature plays a central role in tumor progression, making it an attractive therapeutic target. In this study, we explore the antiangiogenic potential of our melanoma gene therapy approach combining interferon beta (IFN beta) and p19Arf gene transfer. Since these proteins are modulators of tumor vasculature, we explore the impact of IFN beta and p19Arf gene transfer on murine endothelial cells (tEnd). Adenovirus-mediated gene transfer of p19Arf to tEnd cells inhibited proliferation, tube formation, migration, and led to increased expression of genes related to the p53 cell death pathway, yet IFN beta gene transfer had no significant impact on tEnd viability. Alternatively, tEnd cells were exposed to the factors generated by transduced B16 (mouse melanoma) cells using either coculture or conditioned medium. In either case, transduction of B16 cells with the IFN beta vector, whether alone or in combination with p19Arf, resulted in endothelial cell death. Strikingly, treatment of tEnd cells with recombinant IFN beta did not induce death, demonstrating that additional factors produced by B16 cells contributed to the demise of tEnd cells. In this work, we have shown that our melanoma gene therapy strategy produces desirable negative effects on endothelial cells, possibly correlating with antiangiogenic activity.eng
dc.description.sponsorshipSao Paulo Research Foundation [13/25167-5, 15/26580-9, 11/21256-8, 08/55963-0, 11/10656-5]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
dc.publisherMARY ANN LIEBERT, INCeng
dc.relation.ispartofJournal of Interferon and Cytokine Research
dc.subjectendothelial cellseng
dc.subjectinterferon betaeng
dc.subject.otheradenoviral vectoreng
dc.subject.otherstimulated geneseng
dc.subject.othermouse modeleng
dc.titleDistinct Roles of Direct Transduction Versus Exposure to the Tumor Secretome on Murine Endothelial Cells After Melanoma Gene Therapy with Interferon-beta and p19Arfeng
dc.rights.holderCopyright MARY ANN LIEBERT, INCeng
dc.subject.wosBiochemistry & Molecular Biologyeng
dc.subject.wosCell Biologyeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, Aline:Univ Sao Paulo, Fac Med, Inst Canc Estado Sao Paulo, Viral Vector Lab,Ctr Invest Translac Oncol LIM24, Sao Paulo, SP, Brazil; Cristalia, Biotecnol Unidade 1, Itapira, SP, Brazil
hcfmusp.publisher.cityNEW ROCHELLEeng
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Artigos e Materiais de Revistas Científicas - HC/ICESP
Instituto do Câncer do Estado de São Paulo - HC/ICESP

Artigos e Materiais de Revistas Científicas - LIM/24
LIM/24 - Laboratório de Oncologia Experimental

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

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