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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorALVES, Brunna M.
dc.contributor.authorSIQUEIRA, Juliana D.
dc.contributor.authorPRELLWITZ, Isabel M.
dc.contributor.authorBOTELHO, Ornella M.
dc.contributor.authorHORA, Vanusa P. Da
dc.contributor.authorSANABANI, Sabri
dc.contributor.authorRECORDON-PINSON, Patricia
dc.contributor.authorFLEURY, Herve
dc.contributor.authorSOARES, Esmeralda A.
dc.contributor.authorSOARES, Marcelo A.
dc.identifier.citationFRONTIERS IN MICROBIOLOGY, v.10, article ID 749, 11p, 2019
dc.description.abstractApproximately 36.7 million people were living with the human immunodeficiency virus (HIV) at the end of 2016 according to UNAIDS, representing a global prevalence rate of 0.8%. In Brazil, an HIV prevalence of 0.24% has been estimated, which represents approximately 830,000 individuals living with the virus. As a touristic and commercial hub in Latin America, Brazil harbors an elevated HIV genetic variability, further contributed by the selective pressure exerted by the host immune system and by antiretroviral treatment. Through the progress of the next-generation sequencing (NGS) techniques, it has been possible to expand the study of HIV genetic diversity, evolutionary, and epidemic processes, allowing the generation of HIV complete or near full-length genomes (NFLG) and improving the characterization of intra- and interhost diversity of viral populations. Greater sensitivity in the detection of viral recombinant forms represents one of the major improvements associated with this development. It is possible to identify unique or circulating recombinant forms using the near full-length viral genomes with increasing accuracy. It also permits the characterization of multiple viral infections within individual hosts. Previous Brazilian studies using NGS to analyze HIV diversity were able to identify several distinct unique and circulating recombinant forms and evidenced dual infections. These data unveiled unprecedented high rates of viral recombination and highlighted that novel recombinants are continually arising in the Brazilian epidemic. In the pooled analysis depicted in this report, HIV subtypes have been determined from HIV-positive patients in five states of Brazil with some of the highest HIV prevalence, three in the Southeast (Rio de Janeiro, Sao Paulo, and Minas Gerais), one in the Northeast (Pernambuco) and one in the South (Rio Grande do Sul). Combined data analysis showed a significant prevalence of recombinant forms (29%; 101/350), and a similar 26% when only NFLGs were considered. Moreover, the analysis was able to evidence the occurrence of multiple infections in some individuals. Our data highlight the great HIV genetic diversity found in Brazil and unveils a more accurate scenario of the HIV evolutionary dynamics in the region.eng
dc.description.sponsorshipRio de Janeiro State Science Foundation - FAPERJ [E-26/201.331/2014]
dc.description.sponsorshipBrazilian National Research Council - CNPq [454623/2014-3, 460346/2014-8]
dc.description.sponsorshipMSD Avenir [DS-2016-0005]
dc.description.sponsorshipBrazilian Ministry of Health through INCA
dc.publisherFRONTIERS MEDIA SAeng
dc.relation.ispartofFrontiers in Microbiology
dc.subjectgenetic diversityeng
dc.subject.othertransmitted drug-resistanceeng
dc.subject.otherfull-length genomeseng
dc.subject.othermolecular epidemiologyeng
dc.titleEstimating HIV-1 Genetic Diversity in Brazil Through Next-Generation Sequencingeng
dc.rights.holderCopyright FRONTIERS MEDIA SAeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, Brunna M.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil, Juliana D.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil, Isabel M.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil, Ornella M.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil, Vanusa P. Da:UniV Fed Rio Grande, Escola Med, Lab Biol Mol, Rio Grande, RS, Brazil, Patricia:Univ Bordeaux, CNRS MFP UMR 5234, Univ Hosp Bordeaux, Bordeaux, France, Herve:Univ Bordeaux, CNRS MFP UMR 5234, Univ Hosp Bordeaux, Bordeaux, France, Esmeralda A.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil, Marcelo A.:Inst Nacl Canc, Programa Oncovirol, Rio De Janeiro, Brazil; Univ Fed Rio de Janeiro, Dept Genet, Rio De Janeiro, Brazil
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