Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/31974
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | THANGAVEL, Chellappagounder | - |
dc.contributor.author | GOMES, Cristiano M. | - |
dc.contributor.author | ZDERIC, Stephen A. | - |
dc.contributor.author | JAVED, Elham | - |
dc.contributor.author | ADDYA, Sankar | - |
dc.contributor.author | SINGH, Jagmohan | - |
dc.contributor.author | DAS, Sreya | - |
dc.contributor.author | BIRBE, Ruth | - |
dc.contributor.author | DEN, Robert B. | - |
dc.contributor.author | RATTAN, Satish | - |
dc.contributor.author | DESHPANDE, Deepak A. | - |
dc.contributor.author | PENN, Raymond B. | - |
dc.contributor.author | CHACKO, Samuel | - |
dc.contributor.author | BOOPATHI, Ettickan | - |
dc.date.accessioned | 2019-05-30T13:46:57Z | - |
dc.date.available | 2019-05-30T13:46:57Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | AMERICAN JOURNAL OF PATHOLOGY, v.189, n.4, p.847-867, 2019 | - |
dc.identifier.issn | 0002-9440 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/31974 | - |
dc.description.abstract | Caveolins (CAVs) are structural proteins of caveolae that function as signaling platforms to regulate smooth muscle contraction. Loss of CAV protein expression is associated with impaired contraction in obstruction-induced bladder smooth muscle (BSM) hypertrophy. In this study, microarray analysis of bladder RNA revealed down-regulation of CAV1, CAV2, and CAV3 gene transcription in BSM from models of obstructive bladder disease in mice and humans. We identified and characterized regulatory regions responsible for CAV1, CAV2, and CAV3 gene expression in mice with obstruction-induced BSM hypertrophy, and in men with benign prostatic hyperplasia. DNA affinity chromatography and chromatin immunoprecipitation assays revealed a greater increase in binding of GATA-binding factor 6 (GATA-6) and NE-KB to their cognate binding motifs on CAV1, CAV2, and CAV3 promoters in obstructed BSM relative to that observed in control BSM. Knockout of NE-KB subunits, shRNA-mediated knockdown of GATA-6, or pharmacologic inhibition of GATA-6 and NE-KB in BSM increased CAV1, CAV2, and CAV3 transcription and promoter activity. Conversely, overexpression of GATA-6 decreased CAV2 and CAV3 transcription and promoter activity. Collectively, these data provide new insight into the mechanisms by which CAV gene expression is repressed in hypertrophied BSM in obstructive bladder disease. | eng |
dc.description.sponsorship | NIH [R01DK100483, R01NIDDK035385] | - |
dc.description.sponsorship | Thomas Jefferson University Sidney Kimmel Cancer Center [5P30CA056036-17] | - |
dc.language.iso | eng | - |
dc.publisher | ELSEVIER SCIENCE INC | eng |
dc.relation.ispartof | American Journal of Pathology | - |
dc.rights | restrictedAccess | eng |
dc.subject.other | outlet obstruction | eng |
dc.subject.other | gene-expression | eng |
dc.subject.other | murine model | eng |
dc.subject.other | ca2+ sensitization | eng |
dc.subject.other | up-regulation | eng |
dc.subject.other | lipid rafts | eng |
dc.subject.other | map kinase | eng |
dc.subject.other | inhibitor | eng |
dc.subject.other | contractility | eng |
dc.subject.other | calcium | eng |
dc.title | NF-kappa B and GATA-Binding Factor 6 Repress Transcription of Caveolins in Bladder Smooth Muscle Hypertrophy | eng |
dc.type | article | eng |
dc.rights.holder | Copyright ELSEVIER SCIENCE INC | eng |
dc.identifier.doi | 10.1016/j.ajpath.2018.12.013 | - |
dc.identifier.pmid | 30707892 | - |
dc.subject.wos | Pathology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | THANGAVEL, Chellappagounder:Thomas Jefferson Univ, Dept Radiat Oncol, Ctr Translat Med, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | ZDERIC, Stephen A.:Childrens Hosp Philadelphia, Dept Urol, Philadelphia, PA 19104 USA | - |
hcfmusp.author.external | JAVED, Elham:Thomas Jefferson Univ, Dept Med, Ctr Translat Med, Room 541,Jefferson Alumni Hall,1020 Locust St, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | ADDYA, Sankar:Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | SINGH, Jagmohan:Thomas Jefferson Univ, Dept Med, Div Gastroenterol & Hepatol, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | DAS, Sreya:Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | BIRBE, Ruth:Cooper Univ Hlth Care, Dept Pathol & Lab Med, Camden, NJ USA | - |
hcfmusp.author.external | DEN, Robert B.:Thomas Jefferson Univ, Dept Radiat Oncol, Ctr Translat Med, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | RATTAN, Satish:Thomas Jefferson Univ, Dept Med, Div Gastroenterol & Hepatol, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | DESHPANDE, Deepak A.:Thomas Jefferson Univ, Dept Med, Ctr Translat Med, Room 541,Jefferson Alumni Hall,1020 Locust St, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | PENN, Raymond B.:Thomas Jefferson Univ, Dept Med, Ctr Translat Med, Room 541,Jefferson Alumni Hall,1020 Locust St, Philadelphia, PA 19107 USA | - |
hcfmusp.author.external | CHACKO, Samuel:Univ Penn, Div Urol, Philadelphia, PA 19104 USA; Univ Penn, Dept Pathobiol, Philadelphia, PA 19104 USA | - |
hcfmusp.author.external | BOOPATHI, Ettickan:Thomas Jefferson Univ, Dept Med, Ctr Translat Med, Room 541,Jefferson Alumni Hall,1020 Locust St, Philadelphia, PA 19107 USA; Univ Penn, Div Urol, Philadelphia, PA 19104 USA | - |
hcfmusp.description.beginpage | 847 | - |
hcfmusp.description.endpage | 867 | - |
hcfmusp.description.issue | 4 | - |
hcfmusp.description.volume | 189 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000465053100013 | - |
hcfmusp.origem.id | 2-s2.0-85063079229 | - |
hcfmusp.publisher.city | NEW YORK | eng |
hcfmusp.publisher.country | USA | eng |
hcfmusp.relation.reference | Ackers-Johnson M, 2015, ARTERIOSCL THROM VAS, V35, P817, DOI 10.1161/ATVBAHA.114.305218 | eng |
hcfmusp.relation.reference | [Anonymous], 2011, GUIDE CARE USE LAB A | eng |
hcfmusp.relation.reference | Austin JC, 2004, J UROLOGY, V172, P1524, DOI 10.1097/01.ju.0000138045.61378.96 | eng |
hcfmusp.relation.reference | Baeuerle PA, 1996, CELL, V87, P13, DOI 10.1016/S0092-8674(00)81318-5 | eng |
hcfmusp.relation.reference | Balijepalli RC, 2008, PROG BIOPHYS MOL BIO, V98, P149, DOI 10.1016/j.pbiomolbio.2009.01.012 | eng |
hcfmusp.relation.reference | Bastiani M, 2009, J CELL BIOL, V185, P1259, DOI 10.1083/jcb.200903053 | eng |
hcfmusp.relation.reference | Bergdahl A, 2004, CAN J PHYSIOL PHARM, V82, P289, DOI 10.1139/Y04-033 | eng |
hcfmusp.relation.reference | Biederer CH, 2000, J BIOL CHEM, V275, P26245, DOI 10.1074/jbc.M001430200 | eng |
hcfmusp.relation.reference | Bist A, 2000, BIOCHEMISTRY-US, V39, P1966, DOI 10.1021/bi991721h | eng |
hcfmusp.relation.reference | Bist A, 1997, P NATL ACAD SCI USA, V94, P10693, DOI 10.1073/pnas.94.20.10693 | eng |
hcfmusp.relation.reference | Boopathi E, 2013, MOL CELL BIOL, V33, P1085, DOI 10.1128/MCB.00626-12 | eng |
hcfmusp.relation.reference | Boopathi E, 2011, AM J PATHOL, V178, P2236, DOI 10.1016/j.ajpath.2011.01.038 | eng |
hcfmusp.relation.reference | Burch JBE, 2005, SEMIN CELL DEV BIOL, V16, P71, DOI 10.1016/j.semcdb.2004.10.002 | eng |
hcfmusp.relation.reference | Campostrini G, 2017, CARDIOVASC RES, V113, P1256, DOI 10.1093/cvr/cvx122 | eng |
hcfmusp.relation.reference | Cao GW, 2003, AM J PATHOL, V162, P1241, DOI 10.1016/S0002-9440(10)63920-X | eng |
hcfmusp.relation.reference | Chang AY, 2011, AM J PHYSIOL-RENAL, V301, pF813, DOI 10.1152/ajprenal.00586.2010 | eng |
hcfmusp.relation.reference | Chang DF, 2003, DEV CELL, V4, P107, DOI 10.1016/S1534-5807(02)00396-9 | eng |
hcfmusp.relation.reference | Chang SH, 2010, AM J PHYSIOL-RENAL, V298, pF1416, DOI 10.1152/ajprenal.00595.2009 | eng |
hcfmusp.relation.reference | Cohen AW, 2004, PHYSIOL REV, V84, P1341, DOI 10.1152/physre.00046.2003 | eng |
hcfmusp.relation.reference | Cohen AW, 2003, AM J PHYSIOL-CELL PH, V284, pC457, DOI 10.1152/ajpcell.00380.2002 | eng |
hcfmusp.relation.reference | Couet J, 1997, J BIOL CHEM, V272, P30429, DOI 10.1074/jbc.272.48.30429 | eng |
hcfmusp.relation.reference | Couet J, 1997, J BIOL CHEM, V272, P6525, DOI 10.1074/jbc.272.10.6525 | eng |
hcfmusp.relation.reference | Cristofaro V, 2007, NEUROUROL URODYNAM, V26, P71, DOI 10.1002/nau.20361 | eng |
hcfmusp.relation.reference | Darby PJ, 2000, AM J PHYSIOL-LUNG C, V279, pL1226 | eng |
hcfmusp.relation.reference | DIGNAM JD, 1983, NUCLEIC ACIDS RES, V11, P1475, DOI 10.1093/nar/11.5.1475 | eng |
hcfmusp.relation.reference | EVANS T, 1988, P NATL ACAD SCI USA, V85, P5976, DOI 10.1073/pnas.85.16.5976 | eng |
hcfmusp.relation.reference | Fang P, 2016, MOL IMMUNOL, V75, P144, DOI 10.1016/j.molimm.2016.05.017 | eng |
hcfmusp.relation.reference | Fielding CJ, 2003, BBA-BIOMEMBRANES, V1610, P219, DOI 10.1016/S0005-2736(03)00020-8 | eng |
hcfmusp.relation.reference | GABELLA G, 1976, CELL TISSUE RES, V170, P161 | eng |
hcfmusp.relation.reference | Galbiati F, 2001, CELL, V106, P403, DOI 10.1016/S0092-8674(01)00472-X | eng |
hcfmusp.relation.reference | Gosens R, 2007, AM J PHYSIOL-LUNG C, V293, pL1406, DOI 10.1152/ajplung.00312.2007 | eng |
hcfmusp.relation.reference | Gosens R, 2006, AM J PHYSIOL-LUNG C, V291, pL523, DOI 10.1152/ajplung.00013.2006 | eng |
hcfmusp.relation.reference | Gosens R, 2011, J CELL MOL MED, V15, P2430, DOI 10.1111/j.1582-4934.2010.01246.x | eng |
hcfmusp.relation.reference | Gutierrez-Pajares Jorge L, 2015, Front Cardiovasc Med, V2, P27, DOI 10.3389/fcvm.2015.00027 | eng |
hcfmusp.relation.reference | Hill MM, 2008, CELL, V132, P113, DOI 10.1016/j.cell.2007.11.042 | eng |
hcfmusp.relation.reference | Hu SQ, 2012, INT J MOL SCI, V13, P15653, DOI 10.3390/ijms131215653 | eng |
hcfmusp.relation.reference | Huang JH, 2015, P NATL ACAD SCI USA, V112, P4447, DOI 10.1073/pnas.1420363112 | eng |
hcfmusp.relation.reference | Imagawa S, 2003, FASEB J, V17, P1742, DOI 10.1096/fj.02-1134fje | eng |
hcfmusp.relation.reference | Irrera N, 2017, CLIN SCI, V131, P487, DOI 10.1042/CS20160645 | eng |
hcfmusp.relation.reference | Je HD, 2004, AM J PHYSIOL-HEART C, V286, pH91, DOI 10.1152/ajpheart.00472.2003 | eng |
hcfmusp.relation.reference | KADONAGA JT, 1986, P NATL ACAD SCI USA, V83, P5889, DOI 10.1073/pnas.83.16.5889 | eng |
hcfmusp.relation.reference | Kaneko H, 2017, GENES CELLS, V22, P939, DOI 10.1111/gtc.12537 | eng |
hcfmusp.relation.reference | Kaneko H, 2010, CURR OPIN HEMATOL, V17, P163, DOI 10.1097/MOH.0b013e32833800b8 | eng |
hcfmusp.relation.reference | Kanematsu A, 2007, AM J PHYSIOL-CELL PH, V293, pC1093, DOI 10.1152/ajpcell.00225.2007 | eng |
hcfmusp.relation.reference | Karbalaei MS, 2012, EUR J PHARMACOL, V689, P179, DOI 10.1016/j.ejphar.2012.05.023 | eng |
hcfmusp.relation.reference | Karin M, 2002, NAT IMMUNOL, V3, P221, DOI 10.1038/ni0302-221 | eng |
hcfmusp.relation.reference | Keshavarz M, 2017, FRONT PHYSIOL, V8, DOI 10.3389/fphys.2017.00295 | eng |
hcfmusp.relation.reference | Lai HH, 2004, NEUROCHEM INT, V45, P1185, DOI 10.1016/j.neuint.2004.06.016 | eng |
hcfmusp.relation.reference | Lassmann J, 2008, AM J PHYSIOL-REG I, V294, pR58, DOI 10.1152/ajpregu.00477.2007 | eng |
hcfmusp.relation.reference | Lee YH, 2001, EXP PHYSIOL, V86, P283, DOI 10.1113/eph8602184 | eng |
hcfmusp.relation.reference | Lien CL, 1999, DEVELOPMENT, V126, P75 | eng |
hcfmusp.relation.reference | Lin V.K., 1995, ADV EXP MED BIOL, V385, P75 | eng |
hcfmusp.relation.reference | LIN VK, 1995, ADV EXP MED BIOL, V385, P65 | eng |
hcfmusp.relation.reference | Liu PS, 1996, J BIOL CHEM, V271, P10299, DOI 10.1074/jbc.271.17.10299 | eng |
hcfmusp.relation.reference | Lowalekar SK, 2012, NEUROUROL URODYNAM, V31, P586, DOI 10.1002/nau.21217 | eng |
hcfmusp.relation.reference | Martinez-Moreno M, 2007, J BIOL CHEM, V282, P23044, DOI 10.1074/jbc.M610751200 | eng |
hcfmusp.relation.reference | Masternak K, 2000, GENE DEV, V14, P1156 | eng |
hcfmusp.relation.reference | Molkentin JD, 1997, CIRCULATION, V96, P3833 | eng |
hcfmusp.relation.reference | Molkentin JD, 2000, J BIOL CHEM, V275, P38949, DOI 10.1074/jbc.R000029200 | eng |
hcfmusp.relation.reference | Molkentin JD, 1998, CELL, V93, P215, DOI 10.1016/S0092-8674(00)81573-1 | eng |
hcfmusp.relation.reference | Molkentin JD, 2000, MOL CELL BIOL, V20, P5256, DOI 10.1128/MCB.20.14.5256-5260.2000 | eng |
hcfmusp.relation.reference | Moriguchi T, 2015, MOL CELL BIOL, V35, P805, DOI 10.1128/MCB.01011-14 | eng |
hcfmusp.relation.reference | Morrisey EE, 1996, DEV BIOL, V177, P309, DOI 10.1006/dbio.1996.0165 | eng |
hcfmusp.relation.reference | Oh J, 2004, MOL CELL BIOL, V24, P8519, DOI 10.1128/MCB.24.19.8519-8528.2004 | eng |
hcfmusp.relation.reference | ONEILL LP, 1995, EMBO J, V14, P3946, DOI 10.1002/j.1460-2075.1995.tb00066.x | eng |
hcfmusp.relation.reference | ORKIN SH, 1992, BLOOD, V80, P575 | eng |
hcfmusp.relation.reference | Osman Nadir I, 2018, Eur Urol Focus, V4, P6, DOI 10.1016/j.euf.2018.03.012 | eng |
hcfmusp.relation.reference | Osman NI, 2014, EUR UROL, V65, P389, DOI 10.1016/j.eururo.2013.10.015 | eng |
hcfmusp.relation.reference | Patel HH, 2007, FASEB J, V21, P2970, DOI 10.1096/fj.07-8424com | eng |
hcfmusp.relation.reference | Polyak E, 2009, J UROLOGY, V182, P2497, DOI 10.1016/j.juro.2009.07.011 | eng |
hcfmusp.relation.reference | Razani B, 2002, PHARMACOL REV, V54, P431, DOI 10.1124/pr.54.3.431 | eng |
hcfmusp.relation.reference | Rohrmann D, 1996, J UROLOGY, V156, P578, DOI 10.1016/S0022-5347(01)65756-2 | eng |
hcfmusp.relation.reference | Sadegh MK, 2011, BRIT J PHARMACOL, V162, P1156, DOI 10.1111/j.1476-5381.2010.01115.x | eng |
hcfmusp.relation.reference | Shaw J, 2008, P NATL ACAD SCI USA, V105, P20734, DOI 10.1073/pnas.0807735105 | eng |
hcfmusp.relation.reference | Shimizu R, 2016, EXP HEMATOL, V44, P696, DOI 10.1016/j.exphem.2016.05.010 | eng |
hcfmusp.relation.reference | Shirvani S, 2006, BIOCHEM BIOPH RES CO, V339, P151, DOI 10.1016/j.bbrc.2005.10.190 | eng |
hcfmusp.relation.reference | Stehr M, 2004, J UROLOGY, V172, P2451, DOI 10.1097/01.ju.0000138084.53577.ca | eng |
hcfmusp.relation.reference | Stehr M, 2003, J UROLOGY, V169, P1165, DOI 10.1097/01.ju.0000041501.01323.b9 | eng |
hcfmusp.relation.reference | Stein R, 2001, J UROLOGY, V165, P2284, DOI 10.1016/S0022-5347(05)66185-X | eng |
hcfmusp.relation.reference | Stein R, 2001, J UROLOGY, V166, P651, DOI 10.1016/S0022-5347(05)66037-5 | eng |
hcfmusp.relation.reference | Stein R, 2000, J UROLOGY, V164, P1026, DOI 10.1016/S0022-5347(05)67242-4 | eng |
hcfmusp.relation.reference | Sullivan MP, 2012, BJU INT, V110, pE1163, DOI 10.1111/j.1464-410X.2012.11410.x | eng |
hcfmusp.relation.reference | Umetani M, 2000, BIOCHEM BIOPH RES CO, V272, P370, DOI 10.1006/bbrc.2000.2784 | eng |
hcfmusp.relation.reference | Urban NH, 2003, AM J PHYSIOL-CELL PH, V285, pC1377, DOI 10.1152/ajpcell.00501.2002 | eng |
hcfmusp.relation.reference | van Berlo JH, 2010, CIRC RES, V107, P1032, DOI 10.1161/CIRCRESAHA.110.220764 | eng |
hcfmusp.relation.reference | van den Heuvel APJ, 2005, BIOCHEM J, V385, P795, DOI 10.1042/BJ20041449 | eng |
hcfmusp.relation.reference | Vasquez E, 2017, JCI INSIGHT, V2, DOI 10.1172/jci.insight.90617 | eng |
hcfmusp.relation.reference | Wang Y, 2012, P NATL ACAD SCI USA, V109, P4892, DOI 10.1073/pnas.1112129109 | eng |
hcfmusp.relation.reference | Woodman SE, 2004, J UROLOGY, V171, P950, DOI 10.1097/01.ju.0000105102.72295.b8 | eng |
hcfmusp.relation.reference | Xia ZB, 2018, MEDICINE, V97, DOI 10.1097/MD.0000000000010920 | eng |
hcfmusp.relation.reference | Xin M, 2006, P NATL ACAD SCI USA, V103, P11189, DOI 10.1073/pnas.0604604103 | eng |
hcfmusp.relation.reference | Yamamoto M, 1999, EXP CELL RES, V247, P380, DOI 10.1006/excr.1998.4379 | eng |
hcfmusp.relation.reference | Yin F, 2005, J BIOL CHEM, V280, P4745, DOI 10.1074/jbc.M411585200 | eng |
hcfmusp.relation.reference | Zderic SA, 1998, WORLD J UROL, V16, P350, DOI 10.1007/s003450050079 | eng |
hcfmusp.relation.reference | Zderic SA, 2012, J CELL MOL MED, V16, P203, DOI 10.1111/j.1582-4934.2011.01368.x | eng |
hcfmusp.relation.reference | Zhu BY, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0176759 | eng |
dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 1525-2191 | - |
hcfmusp.citation.scopus | 5 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - HC/IOT Artigos e Materiais de Revistas Científicas - LIM/55 Artigos e Materiais de Revistas Científicas - ODS/03 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_THANGAVEL_NFkappa_B_and_GATABinding_Factor_6_Repress_Transcription_2019.PDF Restricted Access | 2.9 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.