Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/32021
Title: | Multiplex-PCR for diagnosis of bacterial meningitis |
Authors: | ALBUQUERQUE, Renata Chaves; MORENO, Ana Carolina Ramos; SANTOS, Silvia Regina dos; RAGAZZI, Selma Lopes Betta; MARTINEZ, Marina Baquerizo |
Citation: | BRAZILIAN JOURNAL OF MICROBIOLOGY, v.50, n.2, p.435-443, 2019 |
Abstract: | Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1pg/L. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - HU Artigos e Materiais de Revistas Científicas - ODS/03 |
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File | Description | Size | Format | |
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art_ALBUQUERQUE_MultiplexPCR_for_diagnosis_of_bacterial_meningitis_2019.PDF | 513.72 kB | Adobe PDF | View/Open |
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