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Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/33108
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTOLEDO, Beatriz
dc.contributor.authorCORRAL, Marcelo A.
dc.contributor.authorMEISEL, Dirce Mary C. L.
dc.contributor.authorGOTTARDI, Maiara
dc.contributor.authorABDALA, Edson
dc.contributor.authorCOSTA, Silvia F.
dc.contributor.authorPIERROTTI, Ligia Camera
dc.contributor.authorLESCANO, Susana A. Z.
dc.contributor.authorGONCALVES, Elenice M. N.
dc.contributor.authorCASTILHO, Vera L. P.
dc.contributor.authorCHIEFFI, Pedro P.
dc.contributor.authorGRYSCHEK, Ronaldo C. B.
dc.contributor.authorPAULA, Fabiana M.
dc.date.accessioned2019-08-20T14:44:53Z
dc.date.available2019-08-20T14:44:53Z
dc.date.issued2019
dc.identifier.citationCLINICS, v.74, article ID e698, 3p, 2019
dc.identifier.issn1807-5932
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/33108
dc.description.abstractOBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.eng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP 2010/51110-2, 2013/04236-9]
dc.language.isoeng
dc.publisherHOSPITAL CLINICAS, UNIV SAO PAULOeng
dc.relation.ispartofClinics
dc.rightsopenAccesseng
dc.subjectHeterologous Antigeneng
dc.subjectStrongyloides Venezuelensiseng
dc.subjectELISA Testeng
dc.subjectTransplant Candidateseng
dc.subject.otherstercoraliseng
dc.subject.otherdiagnosiseng
dc.titleScreening of Strongyloides infection using an ELISA test in transplant candidateseng
dc.typearticleeng
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULOeng
dc.identifier.doi10.6061/clinics/2019/e698
dc.identifier.pmid31188909
dc.subject.wosMedicine, General & Internaleng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalTOLEDO, Beatriz:Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med LIM 06,Lab Imunopatol Esquistossom, Sao Paulo, SP, Brazil
hcfmusp.author.externalGOTTARDI, Maiara:Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med LIM 06,Lab Imunopatol Esquistossom, Sao Paulo, SP, Brazil
hcfmusp.description.articlenumbere698
hcfmusp.description.volume74
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000471295200001
hcfmusp.origem.idSCIELO:S1807-59322019000100235
hcfmusp.origem.id2-s2.0-85068164631
hcfmusp.publisher.citySAO PAULOeng
hcfmusp.publisher.countryBRAZILeng
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dc.description.indexMEDLINEeng
dc.identifier.eissn1980-5322
hcfmusp.citation.scopus12
hcfmusp.scopus.lastupdate2024-03-29-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICESP
Instituto do Câncer do Estado de São Paulo - HC/ICESP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/03
LIM/03 - Laboratório de Medicina Laboratorial

Artigos e Materiais de Revistas Científicas - LIM/06
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses

Artigos e Materiais de Revistas Científicas - LIM/37
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado

Artigos e Materiais de Revistas Científicas - LIM/49
LIM/49 - Laboratório de Protozoologia


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