Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/33491
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLEITE, Gleice Cristina
dc.contributor.authorSTABLER, Richard A.
dc.contributor.authorNEVES, Patricia
dc.contributor.authorPERDIGAO NETO, Lauro V.
dc.contributor.authorMARTINS, Roberta C. Ruedas
dc.contributor.authorRIZEK, Camila
dc.contributor.authorROSSI, Flavia
dc.contributor.authorLEVIN, Anna S.
dc.contributor.authorCOSTA, Silvia Figueiredo
dc.date.accessioned2019-09-23T14:18:19Z
dc.date.available2019-09-23T14:18:19Z
dc.date.issued2019
dc.identifier.citationDIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v.95, n.1, p.99-101, 2019
dc.identifier.issn0732-8893
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/33491
dc.description.abstractTreatment of infections caused by A. baumannii is becoming a challenge due to the ability to develop multidrug-resistance, virulence, and high mortality. We described the colistin resistance and virulence genes present in sixA. baumannii clinical isolates using WGS, expression by qPCR, and virulence in the Galleria mellonella model. The colistin-resistant isolates were assigned as ST233 and the colistin-susceptible isolates as ST236 and ST407. The colistin-resistant isolates contained mutations within PmrA/PmrB, and the pmrA showed up-regulation in all of them. Only one colistin-resistant isolate indicating virulence in G. mellonella. This particular isolate belonged to a different clone, and it was the only isolate that presented non-synonymous mutations in pmrB. Colistinresistance in A. baumannii isolates seems to be caused by up-regulation of pmrA gene. Only one isolate appeared to be virulent in the G. mellonella model. This finding indicating low virulence in isolates belonging to emerging clones circulating in our hospital.eng
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INCeng
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.rightsrestrictedAccesseng
dc.subjectAcinetobacter baumanniieng
dc.subjectGalleria mellonellaeng
dc.subjectwhole-sequencingeng
dc.subjectcolistin-resistanteng
dc.subjectresistanceeng
dc.subjectvirulenceeng
dc.subject.otheracinetobacter-baumanniieng
dc.subject.otheralignmenteng
dc.titleGenetic and virulence characterization of colistin-resistant and colistin-sensitive A. baumannii clinical isolateseng
dc.typearticleeng
dc.rights.holderCopyright ELSEVIER SCIENCE INCeng
dc.identifier.doi10.1016/j.diagmicrobio.2019.04.013
dc.identifier.pmid31178071
dc.subject.wosInfectious Diseaseseng
dc.subject.wosMicrobiologyeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalLEITE, Gleice Cristina:Univ Sao Paulo, Dept Infect Dis, Sao Paulo, Brazil; Lab Med Invest 54 LIM 54, Sao Paulo, Brazil
hcfmusp.author.externalSTABLER, Richard A.:London Sch Hyg & Trop Med, London, England
hcfmusp.author.externalNEVES, Patricia:Univ Sao Paulo, Dept Infect Dis, Sao Paulo, Brazil; Lab Med Invest 54 LIM 54, Sao Paulo, Brazil
hcfmusp.description.beginpage99
hcfmusp.description.endpage101
hcfmusp.description.issue1
hcfmusp.description.volume95
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000483427000021
hcfmusp.origem.id2-s2.0-85066786638
hcfmusp.publisher.cityNEW YORKeng
hcfmusp.publisher.countryUSAeng
hcfmusp.relation.referenceAdams MD, 2009, ANTIMICROB AGENTS CH, V53, P3628, DOI 10.1128/AAC.00284-09eng
hcfmusp.relation.referenceArroyo LA, 2011, ANTIMICROB AGENTS CH, V55, P3743, DOI 10.1128/AAC.00256-11eng
hcfmusp.relation.referenceBiswas S, 2012, EXPERT REV ANTI-INFE, V10, P917, DOI [10.1586/eri.12.78, 10.1586/ERI.12.78]eng
hcfmusp.relation.referenceClinical and Laboratory Standard Institute, 2013, M100 CLSIeng
hcfmusp.relation.referenceDias VC, 2016, J INFECT DEV COUNTR, V10, P544, DOI 10.3855/jidc.6640eng
hcfmusp.relation.referenceGoh HMS, 2013, APPL ENVIRON MICROB, V79, P6535, DOI 10.1128/AEM.01402-13eng
hcfmusp.relation.referenceKatoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010eng
hcfmusp.relation.referenceKavanagh Kevin, 2010, Fungal Biology Reviews, V24, P79, DOI 10.1016/j.fbr.2010.04.001eng
hcfmusp.relation.referenceLeite GC, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0151270eng
hcfmusp.relation.referenceLi H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324eng
hcfmusp.relation.referenceLivak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262eng
hcfmusp.relation.referenceLoehfelm TW, 2008, J BACTERIOL, V190, P1036, DOI 10.1128/JB.01416-07eng
hcfmusp.relation.referenceLopez-Rojas R, 2011, J INFECT DIS, V203, P545, DOI 10.1093/infdis/jiq086eng
hcfmusp.relation.referencePark YK, 2011, INT J ANTIMICROB AG, V37, P525, DOI 10.1016/j.ijantimicag.2011.02.008eng
hcfmusp.relation.referencePfaffl MW, 2004, A Z QUANTITATIVE PCR, P87, DOI 10.1007/S10551-011-0963-1eng
hcfmusp.relation.referenceTomaras AP, 2008, MICROBIOL-SGM, V154, P3398, DOI 10.1099/mic.0.2008/019471-0eng
hcfmusp.relation.referenceWand ME, 2015, J ANTIMICROB CHEMOTH, V70, P2209, DOI 10.1093/jac/dkv097eng
hcfmusp.relation.referenceZarrilli R, 2016, VIRULENCE, V7, P367, DOI 10.1080/21505594.2016.1150405eng
dc.description.indexMEDLINEeng
dc.identifier.eissn1879-0070
hcfmusp.citation.scopus5
hcfmusp.scopus.lastupdate2022-09-23-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/03
LIM/03 - Laboratório de Medicina Laboratorial

Artigos e Materiais de Revistas Científicas - LIM/49
LIM/49 - Laboratório de Protozoologia


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