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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | BANDO, Silvia Y. | |
dc.contributor.author | IAMASHITA, Priscila | |
dc.contributor.author | SILVA, Filipi N. | |
dc.contributor.author | COSTA, Luciano da F. | |
dc.contributor.author | ABE, Cecilia M. | |
dc.contributor.author | BERTONHA, Fernanda B. | |
dc.contributor.author | GUTH, Beatriz E. C. | |
dc.contributor.author | FUJITA, Andre | |
dc.contributor.author | MOREIRA-FILHO, Carlos A. | |
dc.date.accessioned | 2019-09-23T14:19:48Z | - |
dc.date.available | 2019-09-23T14:19:48Z | - |
dc.date.issued | 2019 | |
dc.identifier.citation | MICROORGANISMS, v.7, n.7, article ID 195, 23p, 2019 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/33542 | - |
dc.description.abstract | Shiga toxin-producing Escherichia coli (STEC) O113:H21 strains are associated with human diarrhea and some strains may cause hemolytic-uremic syndrome (HUS). In Brazil, these strains are commonly found in cattle but, so far, were not isolated from HUS patients. Here, a system biology approach was used to investigate the differential transcriptomic and phenotypic responses of enterocyte-like Caco-2 cells to two STEC O113:H21 strains with similar virulence factor profiles (i.e., expressing stx2, ehxA, epeA, espA, iha, saa, sab, and subA): EH41 (Caco-2/EH41), isolated from a HUS patient in Australia, and Ec472/01 (Caco-2/Ec472), isolated from bovine feces in Brazil, during a 3 h period of bacteria-enterocyte interaction. Gene co-expression network analysis for Caco-2/EH41 revealed a quite abrupt pattern of topological variation along 3 h of enterocyte-bacteria interaction when compared with networks obtained for Caco-2/Ec472. Transcriptional module characterization revealed that EH41 induces inflammatory and apoptotic responses in Caco-2 cells just after the first hour of enterocyte-bacteria interaction, whereas the response to Ec472/01 is associated with cytoskeleton organization at the first hour, followed by the expression of immune response modulators. Scanning electron microscopy showed more intense microvilli destruction in Caco-2 cells exposed to EH41 when compared to those exposed to Ec472/01. Altogether, these results show that EH41 expresses virulence genes, inducing a distinctive host cell response, and is likely associated with severe pathogenicity. | eng |
dc.description.sponsorship | Programa de Apoio a Nucleos de Excelencia (PRONEX)/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/50761-2] | |
dc.description.sponsorship | FAPESP [2015/22308-2] | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [307626/2014-8] | |
dc.description.sponsorship | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) | |
dc.language.iso | eng | |
dc.publisher | MDPI | eng |
dc.relation.ispartof | Microorganisms | |
dc.rights | openAccess | eng |
dc.subject | shiga toxin-producing Escherichia coli | eng |
dc.subject | hemolytic-uremic syndrome | eng |
dc.subject | caco-2 cells | eng |
dc.subject | enterocyte-bacteria interaction | eng |
dc.subject | systems biology | eng |
dc.subject | gene co-expression network | eng |
dc.subject | weighted gene co-expression network analysis (WGCNA) | eng |
dc.subject.other | strains | eng |
dc.subject.other | protein | eng |
dc.subject.other | expression | eng |
dc.subject.other | regulator | eng |
dc.subject.other | infection | eng |
dc.subject.other | survival | eng |
dc.subject.other | cluster | eng |
dc.subject.other | marcks | eng |
dc.subject.other | humans | eng |
dc.subject.other | food | eng |
dc.title | Dynamic Gene Network Analysis of Caco-2 Cell Response to Shiga Toxin-Producing Escherichia coli-Associated Hemolytic-Uremic Syndrome | eng |
dc.type | article | eng |
dc.rights.holder | Copyright MDPI | eng |
dc.identifier.doi | 10.3390/microorganisms7070195 | |
dc.identifier.pmid | 31288487 | |
dc.subject.wos | Microbiology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | SILVA, Filipi N.:Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil | |
hcfmusp.author.external | COSTA, Luciano da F.:Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil | |
hcfmusp.author.external | ABE, Cecilia M.:Butantan Inst, Lab Bacteriol, BR-05503900 Sao Paulo, SP, Brazil | |
hcfmusp.author.external | GUTH, Beatriz E. C.:Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, SP, Brazil | |
hcfmusp.author.external | FUJITA, Andre:Univ Sao Paulo, Inst Matemat & Estat, Dept Comp Sci, BR-05508090 Sao Paulo, SP, Brazil | |
hcfmusp.description.articlenumber | 195 | |
hcfmusp.description.issue | 7 | |
hcfmusp.description.volume | 7 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000478618800021 | |
hcfmusp.origem.id | 2-s2.0-85074290060 | |
hcfmusp.publisher.city | BASEL | eng |
hcfmusp.publisher.country | SWITZERLAND | eng |
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dc.description.index | PubMed | eng |
dc.identifier.eissn | 2076-2607 | |
hcfmusp.citation.scopus | 6 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPE Artigos e Materiais de Revistas Científicas - LIM/36 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_BANDO_Dynamic_Gene_Network_Analysis_of_Caco2_Cell_Response_2019.PDF | publishedVersion (English) | 5.13 MB | Adobe PDF | View/Open |
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