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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorZAMBOM, Fernanda Florencia Fregnan
dc.contributor.authorOLIVEIRA, Karin Carneiro
dc.contributor.authorFORESTO-NETO, Orestes
dc.contributor.authorFAUSTINO, Viviane Dias
dc.contributor.authorAVILA, Victor Ferreira
dc.contributor.authorALBINO, Amanda Helen
dc.contributor.authorARIAS, Simone Costa Alarcon
dc.contributor.authorVOLPINI, Rildo Aparecido
dc.contributor.authorMALHEIROS, Denise Maria Avancini Costa
dc.contributor.authorCAMARA, Niels Olsen Saraiva
dc.contributor.authorZATZ, Roberto
dc.contributor.authorFUJIHARA, Clarke Kazue
dc.identifier.citationAMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.317, n.4, p.F1058-F1067, 2019
dc.description.abstractNitric oxide inhibition with N-omega-nitro-L-arginine methyl ester (L-NAME), along with salt overload, leads to hypertension, albuminuria, glomerulosclerosis, glomerular ischemia, and interstitial fibrosis, characterizing a chronic kidney disease (CKD) model. Previous findings of this laboratory and elsewhere have suggested that activation of at least two pathways of innate immunity, Toll-like receptor 4 (TLR4)/NF-kappa B and nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain containing 3 (NLRP3) inflammasome/IL-1 beta, occurs in several experimental models of CKD and that progression of renal injury can be slowed with inhibition of these pathways. In the present study, we investigated whether activation of innate immunity, through either the TLR4/NF-kappa B or NLRP3/IL-1 beta pathway, is involved in the pathogenesis of renal injury in chronic nitric oxide inhibition with the salt-overload model. Adult male Munich-Wistar rats that received L-NAME in drinking water with salt overload (HS + N group) were treated with allopurinol (ALLO) as an NLRP3 inhibitor (HS + N + ALLO group) or pyrrolidine dithiocarbamate (PDTC) as an NF-kappa B inhibitor (HS + N + PDTC group). After 4 wk, HS + N rats developed hypertension, albuminuria, and renal injury along with renal inflammation, oxidative stress, and activation of both the NLRP3/IL-1 beta and TLR4/NF-kappa B pathways. ALLO lowered renal uric acid and inhibited the NLRP3 pathway. These effects were associated with amelioration of hypertension, albuminuria, and interstitial inflammation/fibrosis but not glomerular injury. PDTC inhibited the renal NF-kappa B system and lowered the number of interstitial cells staining positively for NLRP3. PDTC also reduced renal xanthine oxidase activity and uric acid. Overall, PDTC promoted a more efficient anti-inflammatory and nephroprotective effect than ALLO. The NLRP3/IL-1 beta and TLR4/NF-kappa B pathways act in parallel to promote renal injury/inflammation and must be simultaneously inhibited for best nephroprotection.eng
dc.description.sponsorshipSao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2012/10926-5, 2015/25368-6]
dc.description.sponsorshipNational Council for Scientific and Technological Development Award [303684/2013-5]
dc.relation.ispartofAmerican Journal of Physiology-Renal Physiology
dc.subjectchronic kidney diseaseeng
dc.subjecthigh salteng
dc.subjectinnate immunityeng
dc.subjectnitric oxideeng
dc.subject.othervascular smooth-muscleeng
dc.subject.othernitric-oxide synthesiseng
dc.subject.othernlrp3 inflammasome activationeng
dc.subject.othercortical tubulointerstitial injuryeng
dc.subject.otherrenal injuryeng
dc.subject.otheroxidative stresseng
dc.titlePathogenic role of innate immunity in a model of chronic NO inhibition associated with salt overloadeng
dc.rights.holderCopyright AMER PHYSIOLOGICAL SOCeng
dc.subject.wosUrology & Nephrologyeng
dc.type.categoryoriginal articleeng
hcfmusp.relation.referenceArias SCA, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0056215eng
hcfmusp.relation.referenceAnders HJ, 2011, J AM SOC NEPHROL, V22, P1007, DOI 10.1681/ASN.2010080798eng
hcfmusp.relation.referenceArcos MI, 2000, AM J PHYSIOL-RENAL, V279, pF1060eng
hcfmusp.relation.referenceBAYLIS C, 1992, J CLIN INVEST, V90, P278, DOI 10.1172/JCI115849eng
hcfmusp.relation.referenceBomfim GF, 2012, CLIN SCI, V122, P535, DOI 10.1042/CS20110523eng
hcfmusp.relation.referenceBraga TT, 2017, SCI REP-UK, V7, DOI 10.1038/srep39884eng
hcfmusp.relation.referenceBrunner M, 2015, EXCLI J, V14, P439, DOI [10.17179/excli2014-151, 10.17179/excli2015-151]eng
hcfmusp.relation.referenceCorrea-Costa M, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0014298eng
hcfmusp.relation.referenceFang L, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0072344eng
hcfmusp.relation.referenceFaustino VD, 2018, BIOSCIENCE REP, V38, DOI 10.1042/BSR20180762eng
hcfmusp.relation.referenceForesto-Neto O, 2018, LAB INVEST, V98, P773, DOI 10.1038/s41374-018-0029-4eng
hcfmusp.relation.referenceFranco M, 2013, AM J PHYSIOL-RENAL, V304, pF982, DOI 10.1152/ajprenal.00463.2012eng
hcfmusp.relation.referenceFujihara CK, 2006, AM J PHYSIOL-RENAL, V290, pF632, DOI 10.1152/ajprenal.00259.2005eng
hcfmusp.relation.referenceFUJIHARA CK, 1994, AM J PHYSIOL, V266, pF697eng
hcfmusp.relation.referenceFujihara CK, 2001, HYPERTENSION, V37, P170, DOI 10.1161/01.HYP.37.1.170eng
hcfmusp.relation.referenceFujihara CK, 2007, AM J PHYSIOL-RENAL, V292, pF92, DOI 10.1152/ajprenal.00184.2006eng
hcfmusp.relation.referenceGupta SC, 2010, BBA-GENE REGUL MECH, V1799, P775, DOI 10.1016/j.bbagrm.2010.05.004eng
hcfmusp.relation.referenceHan YQ, 1999, CIRC RES, V84, P695, DOI 10.1161/01.RES.84.6.695eng
hcfmusp.relation.referenceHarrison R, 2002, FREE RADICAL BIO MED, V33, P774, DOI 10.1016/S0891-5849(02)00956-5eng
hcfmusp.relation.referenceHerrington FD, 2016, J BIOMOL SCREEN, V21, P223, DOI 10.1177/1087057115617456eng
hcfmusp.relation.referenceHu QH, 2012, BIOCHEM PHARMACOL, V84, P113, DOI 10.1016/j.bcp.2012.03.005eng
hcfmusp.relation.referenceIsaka Y, 2016, NEPHROL DIAL TRANSPL, V31, P890, DOI 10.1093/ndt/gfv024eng
hcfmusp.relation.referenceIshola DA, 2006, KIDNEY INT, V70, P724, DOI 10.1038/
hcfmusp.relation.referenceIves A, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7555eng
hcfmusp.relation.referenceJanssen-Heininger YMW, 2000, FREE RADICAL BIO MED, V28, P1317, DOI 10.1016/S0891-5849(00)00218-5eng
hcfmusp.relation.referenceJEPSEN FL, 1979, VIRCHOWS ARCH A, V383, P265, DOI 10.1007/BF00430245eng
hcfmusp.relation.referenceJi YY, 2009, CELL PHYSIOL BIOCHEM, V23, P265, DOI 10.1159/000218173eng
hcfmusp.relation.referenceKang DH, 2002, J AM SOC NEPHROL, V13, P2888, DOI 10.1097/01.ASN.0000034910.58454.FDeng
hcfmusp.relation.referenceKim SM, 2015, AM J PHYSIOL-RENAL, V308, pF993, DOI 10.1152/ajprenal.00637.2014eng
hcfmusp.relation.referenceKobayashi S, 2003, BIOCHEM BIOPH RES CO, V308, P306, DOI 10.1016/S0006-291X(03)01362-7eng
hcfmusp.relation.referenceLiu HF, 2017, AM J NEPHROL, V46, P333, DOI 10.1159/000481668eng
hcfmusp.relation.referenceLiu SF, 1999, CIRCULATION, V100, P1330, DOI 10.1161/01.CIR.100.12.1330eng
hcfmusp.relation.referenceLiu Y., 2018, COMPLEXITY, V2018, P1, DOI 10.1080/15548627.2018.1531196eng
hcfmusp.relation.referenceMancini G, 1965, Immunochemistry, V2, P235, DOI 10.1016/0019-2791(65)90004-2eng
hcfmusp.relation.referenceMao KR, 2013, CELL RES, V23, P201, DOI 10.1038/cr.2013.6eng
hcfmusp.relation.referenceMartinez FO, 2009, ANNU REV IMMUNOL, V27, P451, DOI 10.1146/annurev.immunol.021908.132532eng
hcfmusp.relation.referenceMartinon F, 2002, MOL CELL, V10, P417, DOI 10.1016/S1097-2765(02)00599-3eng
hcfmusp.relation.referenceMatesanz N, 2007, J HYPERTENS, V25, P609, DOI 10.1097/HJH.0b013e328013e7c4eng
hcfmusp.relation.referenceMazzali M, 2001, HYPERTENSION, V38, P1101, DOI 10.1161/hy1101.092839eng
hcfmusp.relation.referenceMezzano SA, 2001, KIDNEY INT, V60, P1366, DOI 10.1046/j.1523-1755.2001.00941.xeng
hcfmusp.relation.referenceMODOLELL M, 1995, EUR J IMMUNOL, V25, P1101, DOI 10.1002/eji.1830250436eng
hcfmusp.relation.referenceMorgan MJ, 2011, CELL RES, V21, P103, DOI 10.1038/cr.2010.178eng
hcfmusp.relation.referenceMorigi M, 2002, J AM SOC NEPHROL, V13, P1179eng
hcfmusp.relation.referenceMuller DN, 2000, HYPERTENSION, V35, P193, DOI 10.1161/01.HYP.35.1.193eng
hcfmusp.relation.referenceNair AR, 2015, EXP CELL RES, V335, P238, DOI 10.1016/j.yexcr.2015.05.011eng
hcfmusp.relation.referenceOdegaard JI, 2011, ANNU REV PATHOL-MECH, V6, P275, DOI 10.1146/annurev-pathol-011110-130138eng
hcfmusp.relation.referenceOkabe C, 2013, AM J PHYSIOL-RENAL, V305, pF155, DOI 10.1152/ajprenal.00491.2012eng
hcfmusp.relation.referencePekarova M, 2011, THESCIENTIFICWORLDJO, V11, P2443, DOI 10.1100/2011/321979eng
hcfmusp.relation.referencePellegrini C, 2018, FRONT PHARMACOL, V9, DOI 10.3389/fphar.2018.01405eng
hcfmusp.relation.referencePushpakumar S, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-06484-6eng
hcfmusp.relation.referenceQuiroz Y, 2001, AM J PHYSIOL-RENAL, V281, pF38eng
hcfmusp.relation.referenceRangan GK, 2001, RENAL FAILURE, V23, P773, DOI 10.1081/JDI-100108188eng
hcfmusp.relation.referenceRangan GK, 1999, KIDNEY INT, V56, P118, DOI 10.1046/j.1523-1755.1999.00529.xeng
hcfmusp.relation.referenceRIBEIRO MO, 1992, HYPERTENSION, V20, P298, DOI 10.1161/01.HYP.20.3.298eng
hcfmusp.relation.referenceRicardo SD, 2008, J CLIN INVEST, V118, P3522, DOI 10.1172/JCI36150eng
hcfmusp.relation.referenceRincon J, 2015, LIFE SCI, V124, P81, DOI 10.1016/j.lfs.2015.01.005eng
hcfmusp.relation.referenceRodriguez-Iturbe B, 2012, CLIN EXP PHARMACOL P, V39, P96, DOI 10.1111/j.1440-1681.2011.05482.xeng
hcfmusp.relation.referenceSainz J, 2005, AM J HYPERTENS, V18, P871, DOI 10.1016/j.amjhyper.2004.12.022eng
hcfmusp.relation.referenceSATRIANO J, 1994, J CLIN INVEST, V94, P1629, DOI 10.1172/JCI117505eng
hcfmusp.relation.referenceSCHRECK R, 1992, J EXP MED, V175, P1181, DOI 10.1084/jem.175.5.1181eng
hcfmusp.relation.referenceSica A, 2012, J CLIN INVEST, V122, P787, DOI 10.1172/JCI59643eng
hcfmusp.relation.referenceSollinger D, 2014, CARDIOVASC RES, V101, P464, DOI 10.1093/cvr/cvt265eng
hcfmusp.relation.referenceTapia E, 2008, AM J PHYSIOL-RENAL, V295, pF1431, DOI 10.1152/ajprenal.90201.2008eng
hcfmusp.relation.referenceTsai PY, 2011, FREE RADICAL BIO MED, V51, P744, DOI 10.1016/j.freeradbiomed.2011.05.016eng
hcfmusp.relation.referenceVilaysane A, 2010, J AM SOC NEPHROL, V21, P1732, DOI 10.1681/ASN.2010020143eng
hcfmusp.relation.referenceWagner CT, 1997, J CLIN INVEST, V100, P589, DOI 10.1172/JCI119569eng
hcfmusp.relation.referenceWALLENSTEIN S, 1980, CIRC RES, V47, P1, DOI 10.1161/01.RES.47.1.1eng
hcfmusp.relation.referenceWang C, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0038285eng
hcfmusp.relation.referenceWang LJ, 1998, AM J PATHOL, V152, P711eng
hcfmusp.relation.referenceXu P, 1996, GENOMICS, V34, P173, DOI 10.1006/geno.1996.0262eng
hcfmusp.relation.referenceYang Y, 2019, CELL DEATH DIS, V10, DOI 10.1038/s41419-019-1413-8eng
hcfmusp.relation.referenceZhai JX, 2012, MOL BIOL REP, V39, P6763, DOI 10.1007/s11033-012-1501-7eng
hcfmusp.relation.referenceZhao MM, 2011, KIDNEY INT, V79, P1071, DOI 10.1038/ki.2011.18eng
hcfmusp.relation.referenceZhu Q, 2016, AM J PHYSIOL-RENAL, V310, pF621, DOI 10.1152/ajprenal.00344.2015eng
hcfmusp.relation.referenceZhuang YB, 2015, AM J PHYSIOL-RENAL, V308, pF857, DOI 10.1152/ajprenal.00203.2014eng
hcfmusp.relation.referenceZoja C, 1998, KIDNEY INT, V53, P1608, DOI 10.1046/j.1523-1755.1998.00905.xeng
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Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/05
LIM/05 - Laboratório de Poluição Atmosférica Experimental

Artigos e Materiais de Revistas Científicas - LIM/12
LIM/12 - Laboratório de Pesquisa Básica em Doenças Renais

Artigos e Materiais de Revistas Científicas - LIM/16
LIM/16 - Laboratório de Fisiopatologia Renal

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

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