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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCASOTTI, Marcia Oliveira
dc.contributor.authorGRYSCHEK, Ronaldo Cesar Borges
dc.contributor.authorPAULA, Fabiana Martins de
dc.contributor.authorGOMES-GOUVEA, Michele
dc.contributor.authorPINHO, Joao Renato Rebello
dc.contributor.authorTUAN, Roseli
dc.contributor.authorDIAS-NETO, Emmanuel
dc.contributor.authorLUNA, Expedito Jose de Albuquerque
dc.contributor.authorESPIRITO-SANTO, Maria Cristina Carvalho do
dc.date.accessioned2020-06-01T14:53:04Z
dc.date.available2020-06-01T14:53:04Z
dc.date.issued2020
dc.identifier.citationREVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.62, article ID e17, 4p, 2020
dc.identifier.issn0036-4665
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/35972
dc.description.abstractApproximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health. but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission.eng
dc.description.sponsorshipResearch Support Foundation of Sao Paulo State (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/237420]
dc.language.isoeng
dc.publisherINST MEDICINA TROPICAL SAO PAULOeng
dc.relation.ispartofRevista do Instituto de Medicina Tropical de Sao Paulo
dc.rightsopenAccesseng
dc.subjectSchistosoma mansonieng
dc.subjectBiomphalaria glabrataeng
dc.subjectMolecular diagnosiseng
dc.subjectSurveillanceeng
dc.subjectSnaileng
dc.subjectVectoreng
dc.subjectMolecular diagnosiseng
dc.subjectPolymerase Chain Reactioneng
dc.subject.othermodeleng
dc.subject.otherdnaeng
dc.titleMolecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectorseng
dc.typearticleeng
dc.rights.holderCopyright INST MEDICINA TROPICAL SAO PAULOeng
dc.identifier.doi10.1590/S1678-9946202062017
dc.identifier.pmid32130357
dc.subject.wosInfectious Diseaseseng
dc.subject.wosParasitologyeng
dc.subject.wosTropical Medicineeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalTUAN, Roseli:Superintendencia Controle Endemias Estado Sao Paul, Sao Paulo, SP, Brazil
hcfmusp.description.articlenumbere17
hcfmusp.description.volume62
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000518812000002
hcfmusp.origem.id2-s2.0-85081232444
hcfmusp.origem.idSCIELO:S0036-46652020000100209
hcfmusp.publisher.citySAO PAULOeng
hcfmusp.publisher.countryBRAZILeng
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dc.description.indexMEDLINEeng
dc.identifier.eissn1678-9946
hcfmusp.citation.scopus1-
hcfmusp.scopus.lastupdate2024-02-23-
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