https://observatorio.fm.usp.br/handle/OPI/35972
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | CASOTTI, Marcia Oliveira | |
dc.contributor.author | GRYSCHEK, Ronaldo Cesar Borges | |
dc.contributor.author | PAULA, Fabiana Martins de | |
dc.contributor.author | GOMES-GOUVEA, Michele | |
dc.contributor.author | PINHO, Joao Renato Rebello | |
dc.contributor.author | TUAN, Roseli | |
dc.contributor.author | DIAS-NETO, Emmanuel | |
dc.contributor.author | LUNA, Expedito Jose de Albuquerque | |
dc.contributor.author | ESPIRITO-SANTO, Maria Cristina Carvalho do | |
dc.date.accessioned | 2020-06-01T14:53:04Z | |
dc.date.available | 2020-06-01T14:53:04Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.62, article ID e17, 4p, 2020 | |
dc.identifier.issn | 0036-4665 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/35972 | |
dc.description.abstract | Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health. but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission. | eng |
dc.description.sponsorship | Research Support Foundation of Sao Paulo State (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/237420] | |
dc.language.iso | eng | |
dc.publisher | INST MEDICINA TROPICAL SAO PAULO | eng |
dc.relation.ispartof | Revista do Instituto de Medicina Tropical de Sao Paulo | |
dc.rights | openAccess | eng |
dc.subject | Schistosoma mansoni | eng |
dc.subject | Biomphalaria glabrata | eng |
dc.subject | Molecular diagnosis | eng |
dc.subject | Surveillance | eng |
dc.subject | Snail | eng |
dc.subject | Vector | eng |
dc.subject | Molecular diagnosis | eng |
dc.subject | Polymerase Chain Reaction | eng |
dc.subject.other | model | eng |
dc.subject.other | dna | eng |
dc.title | Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors | eng |
dc.type | article | eng |
dc.rights.holder | Copyright INST MEDICINA TROPICAL SAO PAULO | eng |
dc.identifier.doi | 10.1590/S1678-9946202062017 | |
dc.identifier.pmid | 32130357 | |
dc.subject.wos | Infectious Diseases | eng |
dc.subject.wos | Parasitology | eng |
dc.subject.wos | Tropical Medicine | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | TUAN, Roseli:Superintendencia Controle Endemias Estado Sao Paul, Sao Paulo, SP, Brazil | |
hcfmusp.description.articlenumber | e17 | |
hcfmusp.description.volume | 62 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000518812000002 | |
hcfmusp.origem.id | 2-s2.0-85081232444 | |
hcfmusp.origem.id | SCIELO:S0036-46652020000100209 | |
hcfmusp.publisher.city | SAO PAULO | eng |
hcfmusp.publisher.country | BRAZIL | eng |
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dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 1678-9946 | |
hcfmusp.citation.scopus | 1 | - |
hcfmusp.scopus.lastupdate | 2024-02-23 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - FM/MPR Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/03 Artigos e Materiais de Revistas Científicas - LIM/06 Artigos e Materiais de Revistas Científicas - LIM/07 Artigos e Materiais de Revistas Científicas - LIM/27 Artigos e Materiais de Revistas Científicas - LIM/52 |
File | Description | Size | Format | |
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art_CASOTTI_Molecular_detection_of_prepatent_Schistosoma_mansoni_infection_in_2020.PDF | publishedVersion (English) | 416.29 kB | Adobe PDF | View/Open |
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