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Title: An in vivo image acquisition system for the evaluation of tracheal mechanics in rats
Authors: VALENGA, Marcelo HenriqueVITORASSO, Renato de LimaRODRIGUES, Thiago GuimaraesPAZETTI, RogerioCARDOSO, Paulo Francisco GuerreiroMORIYA, Henrique TakachiAOKI, Fabio Gava
Citation: ARTIFICIAL ORGANS, v.44, n.5, p.504-512, 2020
Abstract: Mechanical evaluation of tracheal grafts is of great relevance for transplant research. Although there are some publications demonstrating different techniques of tracheal mechanical evaluation, there is still no definitive or preferred protocol available. Here, we present a simple image processing acquisition system that can be used for in vivo experiments. Six male Wistar rats were submitted to orotracheal intubation and a longitudinal incision was made to expose the trachea. Images of tracheae were acquired from a video camera in different scenarios of bronchoconstriction using methacholine (MCh) (Basal, PBS, MCh 30 mu g/kg, MCh 300 mu g/kg, and postmetabolized) during imposed-inspiration and imposed-expiration. The area variation ratio (the ratio between areas during expiration vs. inspiration) was 1.1x for the Basal group, while the ratio for MCh 300 mu g/kg was 6.5x. The area variation of imaged tracheae was statistically significant at the dose of MCh 300 mu g/kg for imposed-inspiration versus imposed-expiration (P = .002). Likewise, elastance data of respiratory mechanics indicated a statistically significant difference at the dose of MCh 300 mu g/kg for imposed-inspiration versus imposed-expiration (P = .026). Our image processing analysis protocol presented corresponding behavior when compared to mechanical parameters of the respiratory system. In addition, our image acquisition system was able to highlight the differences between imposed-inspiration and imposed-expiration. Image analysis of the tracheal area variation seems to be in agreement with the elastance of the respiratory system. Taken together, these observations may help future studies of tracheal transplantation for in situ assessment of graft patency.
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Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/61
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica

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