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Title: | An effective CTL peptide vaccine for Ebola Zaire Based on Survivors' CD8+targeting of a particular nucleocapsid protein epitope with potential implications for COVID-19 vaccine design |
Authors: | V, C. Herst; BURKHOLZ, S.; SIDNEY, J.; SETTE, A.; HARRIS, P. E.; MASSEY, S.; BRASEL, T.; CUNHA-NETO, E.; ROSA, D. S.; CHAO, W. C. H.; CARBACK, R.; HODGE, T.; WANG, L.; CIOTLOS, S.; LLOYD, P.; RUBSAMEN, R. |
Citation: | VACCINE, v.38, n.28, p.4464-4475, 2020 |
Abstract: | The 2013-2016 West Africa EBOV epidemic was the biggest EBOV outbreak to date. An analysis of virus-specific CD8+ T-cell immunity in 30 survivors showed that 26 of those individuals had a CD8+ response to at least one EBOV protein. The dominant response (25/26 subjects) was specific to the EBOV nucleocapsid protein (NP). It has been suggested that epitopes on the EBOV NP could form an important part of an effective T-cell vaccine for Ebola Zaire. We show that a 9-amino-acid peptide NP44-52 (YQVNNLEEI) located in a conserved region of EBOV NP provides protection against morbidity and mortality after mouse adapted EBOV challenge. A single vaccination in a C57BL/6 mouse using an adjuvanted microsphere peptide vaccine formulation containing NP44-52 is enough to confer immunity in mice. Our work suggests that a peptide vaccine based on CD8+ T-cell immunity in EBOV survivors is conceptually sound and feasible. Nucleocapsid proteins within SARS-CoV-2 contain multiple Class I epitopes with predicted HLA restrictions consistent with broad population coverage. A similar approach to a CTL vaccine design may be possible for that virus. (C) 2020 The Author(s). |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - COVID-19 Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/19 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_V_An_effective_CTL_peptide_vaccine_for_Ebola_Zaire_2020.PDF | publishedVersion (English) | 2.21 MB | Adobe PDF | View/Open |
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