Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/37054
Title: Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance
Authors: CUNHA, Mariana SequetinLUCHS, AdrianaSANTOS, Fabiana Cristina Pereira dosCALEIRO, Giovana SantosNOGUEIRA, Mauricio LacerdaMAIORKA, Paulo Cesar
Citation: ARCHIVES OF VIROLOGY, v.165, n.8, p.1863-1868, 2020
Abstract: The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/Outros
Outros departamentos - FM/Outros

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia


Files in This Item:
File Description SizeFormat 
art_CUNHA_Applying_a_panflavivirus_RTqPCR_assay_in_Brazilian_public_2020.PDF.pdf
  Restricted Access
publishedVersion (English)658.44 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.