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Título: Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance
Autor: CUNHA, Mariana SequetinLUCHS, AdrianaSANTOS, Fabiana Cristina Pereira dosCALEIRO, Giovana SantosNOGUEIRA, Mauricio LacerdaMAIORKA, Paulo Cesar
Citación: ARCHIVES OF VIROLOGY, v.165, n.8, p.1863-1868, 2020
Resumen: The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.
Aparece en las colecciones:

Artigos e Materiais de Revistas Científicas - FM/Outros
Outros departamentos - FM/Outros

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia


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