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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCUNHA, Mariana Sequetin
dc.contributor.authorLUCHS, Adriana
dc.contributor.authorSANTOS, Fabiana Cristina Pereira dos
dc.contributor.authorCALEIRO, Giovana Santos
dc.contributor.authorNOGUEIRA, Mauricio Lacerda
dc.contributor.authorMAIORKA, Paulo Cesar
dc.date.accessioned2020-08-20T13:26:15Z-
dc.date.available2020-08-20T13:26:15Z-
dc.date.issued2020
dc.identifier.citationARCHIVES OF VIROLOGY, v.165, n.8, p.1863-1868, 2020
dc.identifier.issn0304-8608
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/37054-
dc.description.abstractThe aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.eng
dc.description.sponsorshipFAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/23645-4, 2013/21719-3]
dc.language.isoeng
dc.publisherSPRINGER WIENeng
dc.relation.ispartofArchives of Virology
dc.rightsrestrictedAccesseng
dc.subject.otherinsect-specific flaviviruseseng
dc.subject.otherzika viruseng
dc.subject.otheridentificationeng
dc.subject.otherarboviruseng
dc.subject.othermosquitoseng
dc.subject.otherpolymeraseeng
dc.subject.otherstateeng
dc.subject.othergenuseng
dc.subject.otherrnaeng
dc.titleApplying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillanceeng
dc.typearticleeng
dc.rights.holderCopyright SPRINGER WIENeng
dc.identifier.doi10.1007/s00705-020-04680-w
dc.identifier.pmid32474687
dc.subject.wosVirologyeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalCUNHA, Mariana Sequetin:Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo, Brazil
hcfmusp.author.externalLUCHS, Adriana:Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo, Brazil
hcfmusp.author.externalSANTOS, Fabiana Cristina Pereira dos:Adolfo Lutz Inst, Virol Ctr, Resp Dis Lab, Sao Paulo, Brazil
hcfmusp.author.externalNOGUEIRA, Mauricio Lacerda:Fac Med Sao Jose do Rio Preto FAMERP, Sao Paulo, SP, Brazil
hcfmusp.author.externalMAIORKA, Paulo Cesar:Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo, Brazil
hcfmusp.description.beginpage1863
hcfmusp.description.endpage1868
hcfmusp.description.issue8
hcfmusp.description.volume165
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000536450600003
hcfmusp.origem.id2-s2.0-85086168055
hcfmusp.publisher.cityWIENeng
hcfmusp.publisher.countryAUSTRIAeng
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dc.description.indexMEDLINEeng
dc.identifier.eissn1432-8798
hcfmusp.citation.scopus4-
hcfmusp.scopus.lastupdate2022-06-03-
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