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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorCUNHA, Mariana Sequetin
dc.contributor.authorLUCHS, Adriana
dc.contributor.authorSANTOS, Fabiana Cristina Pereira dos
dc.contributor.authorCALEIRO, Giovana Santos
dc.contributor.authorNOGUEIRA, Mauricio Lacerda
dc.contributor.authorMAIORKA, Paulo Cesar
dc.identifier.citationARCHIVES OF VIROLOGY, v.165, n.8, p.1863-1868, 2020
dc.description.abstractThe aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.eng
dc.description.sponsorshipFAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/23645-4, 2013/21719-3]
dc.publisherSPRINGER WIENeng
dc.relation.ispartofArchives of Virology
dc.subject.otherinsect-specific flaviviruseseng
dc.subject.otherzika viruseng
dc.titleApplying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillanceeng
dc.rights.holderCopyright SPRINGER WIENeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, Mariana Sequetin:Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo, Brazil, Adriana:Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo, Brazil, Fabiana Cristina Pereira dos:Adolfo Lutz Inst, Virol Ctr, Resp Dis Lab, Sao Paulo, Brazil, Mauricio Lacerda:Fac Med Sao Jose do Rio Preto FAMERP, Sao Paulo, SP, Brazil, Paulo Cesar:Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo, Brazil
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Artigos e Materiais de Revistas Científicas - FM/Outros
Outros departamentos - FM/Outros

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

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