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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | CUNHA, Mariana Sequetin | |
dc.contributor.author | LUCHS, Adriana | |
dc.contributor.author | SANTOS, Fabiana Cristina Pereira dos | |
dc.contributor.author | CALEIRO, Giovana Santos | |
dc.contributor.author | NOGUEIRA, Mauricio Lacerda | |
dc.contributor.author | MAIORKA, Paulo Cesar | |
dc.date.accessioned | 2020-08-20T13:26:15Z | - |
dc.date.available | 2020-08-20T13:26:15Z | - |
dc.date.issued | 2020 | |
dc.identifier.citation | ARCHIVES OF VIROLOGY, v.165, n.8, p.1863-1868, 2020 | |
dc.identifier.issn | 0304-8608 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/37054 | - |
dc.description.abstract | The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses. | eng |
dc.description.sponsorship | FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/23645-4, 2013/21719-3] | |
dc.language.iso | eng | |
dc.publisher | SPRINGER WIEN | eng |
dc.relation.ispartof | Archives of Virology | |
dc.rights | restrictedAccess | eng |
dc.subject.other | insect-specific flaviviruses | eng |
dc.subject.other | zika virus | eng |
dc.subject.other | identification | eng |
dc.subject.other | arbovirus | eng |
dc.subject.other | mosquitos | eng |
dc.subject.other | polymerase | eng |
dc.subject.other | state | eng |
dc.subject.other | genus | eng |
dc.subject.other | rna | eng |
dc.title | Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance | eng |
dc.type | article | eng |
dc.rights.holder | Copyright SPRINGER WIEN | eng |
dc.identifier.doi | 10.1007/s00705-020-04680-w | |
dc.identifier.pmid | 32474687 | |
dc.subject.wos | Virology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | CUNHA, Mariana Sequetin:Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo, Brazil | |
hcfmusp.author.external | LUCHS, Adriana:Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo, Brazil | |
hcfmusp.author.external | SANTOS, Fabiana Cristina Pereira dos:Adolfo Lutz Inst, Virol Ctr, Resp Dis Lab, Sao Paulo, Brazil | |
hcfmusp.author.external | NOGUEIRA, Mauricio Lacerda:Fac Med Sao Jose do Rio Preto FAMERP, Sao Paulo, SP, Brazil | |
hcfmusp.author.external | MAIORKA, Paulo Cesar:Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo, Brazil | |
hcfmusp.description.beginpage | 1863 | |
hcfmusp.description.endpage | 1868 | |
hcfmusp.description.issue | 8 | |
hcfmusp.description.volume | 165 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000536450600003 | |
hcfmusp.origem.id | 2-s2.0-85086168055 | |
hcfmusp.publisher.city | WIEN | eng |
hcfmusp.publisher.country | AUSTRIA | eng |
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dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 1432-8798 | |
hcfmusp.citation.scopus | 6 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/Outros Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/52 |
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