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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | KANASHIRO-GALO, Luciane | |
dc.contributor.author | ALEXANDRE, Ariane Fernandes | |
dc.contributor.author | TAFURI, Wagner Luiz | |
dc.contributor.author | BARBOZA, Tania Cristina | |
dc.contributor.author | QUARESMA, Juarez Antonio Simoes | |
dc.contributor.author | BRITO, Arival Cardoso de | |
dc.contributor.author | NASCIMENTO, Gabriela Yasmin Francisca da Silva do | |
dc.contributor.author | SANTOS FILHO, Antonio Marques dos | |
dc.contributor.author | SOTTO, Mirian Nacagami | |
dc.contributor.author | PAGLIARI, Carla | |
dc.date.accessioned | 2020-12-16T14:54:53Z | - |
dc.date.available | 2020-12-16T14:54:53Z | - |
dc.date.issued | 2020 | |
dc.identifier.citation | REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.62, article ID e75, 5p, 2020 | |
dc.identifier.issn | 0036-4665 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/38464 | - |
dc.description.abstract | Lacaziosis is a cutaneous mycosis caused by the fungus Lacazia loboi, described in different countries of Latin America and prevalent in the Amazon region. The ineffective immune response against the agent seems to be related to a Th2 pattern of cytokines. There are few reports exploring elements of the humoral response in these lesions. Our aim was to investigate some elements focusing on 13 cells, plasma cells and local expression of IgG and IgM antibodies. Forty skin biopsies of lower limbs were selected. The diagnosis of lacaziosis was based on direct mycological examination and histological analysis. The visualization of fungal cells was improved by using Gridley's staining. An immunohistochemical protocol was performed to detect the expression of B cells, plasma cells. IgG and IgM. A double staining was performed to explore the presence of yeasts in the cytoplasm of keratinocytes, using an anti-AE1 AE3 antibody over Gridley's staining. The inflammatory infiltrate consisted of macrophages, multinucleated giant cells, lymphocytes, and fibrosis. Fungal cells were frequent in the stratum corneum and in both, the dermis and, in 50% of the specimens, also in the epidermis. Cells expressing IgG were more abundant when compared to cells expressing IgM. B cells and the presence of IgG might indicate that the humoral response promotes a Th2 immune response resulting in an anti-inflammatory phenotype. Our results lead us to suggest a possible role of B cells and immunoglobulins in the mechanisms of lacaziosis pathogenesis. | eng |
dc.description.sponsorship | National Council for Scientific and Technological Development (CNPq)National Council for Scientific and Technological Development (CNPq) [470512/2013-0] | |
dc.description.sponsorship | National Council for Scientific and Technological DevelopmentNational Council for Scientific and Technological Development (CNPq) | |
dc.description.sponsorship | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES -Ministry of Education, Brazil)CAPES | |
dc.description.sponsorship | Research Scientific and Development Support Foundation of Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) | |
dc.language.iso | eng | |
dc.publisher | INST MEDICINA TROPICAL SAO PAULO | eng |
dc.relation.ispartof | Revista do Instituto de Medicina Tropical de Sao Paulo | |
dc.rights | openAccess | eng |
dc.subject | Lacaziosis | eng |
dc.subject | Humoral response | eng |
dc.subject | Skin | eng |
dc.subject | Human lesions | eng |
dc.subject | Immunohistochemistry | eng |
dc.subject.other | infection | eng |
dc.subject.other | cells | eng |
dc.title | Lacaziosis: immunohistochemical evaluation of elements of the humoral response in cutaneous lesions | eng |
dc.type | article | eng |
dc.rights.holder | Copyright INST MEDICINA TROPICAL SAO PAULO | eng |
dc.identifier.doi | 10.1590/S1678-9946202062075 | |
dc.identifier.pmid | 33053144 | |
dc.subject.wos | Infectious Diseases | eng |
dc.subject.wos | Parasitology | eng |
dc.subject.wos | Tropical Medicine | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | ALEXANDRE, Ariane Fernandes:Univ Sao Paulo, Fac Med, Dept Patol, Av Dr Arnaldo 455,Sale 1118, BR-01246903 Sao Paulo, SP, Brazil; Inst Assistencia Med Servidor Publ Estadual, Programa Posgrad Ciencias Saude, Grad Ciencias SaUde, Sao Paulo, SP, Brazil | |
hcfmusp.author.external | TAFURI, Wagner Luiz:Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol Geral, Belo Horizonte, MG, Brazil | |
hcfmusp.author.external | BARBOZA, Tania Cristina:Univ Sao Paulo, Fac Med, Dept Patol, Av Dr Arnaldo 455,Sale 1118, BR-01246903 Sao Paulo, SP, Brazil; Inst Assistencia Med Servidor Publ Estadual, Programa Posgrad Ciencias Saude, Grad Ciencias SaUde, Sao Paulo, SP, Brazil | |
hcfmusp.author.external | QUARESMA, Juarez Antonio Simoes:Univ Fed Para, NUcleo Med Trop, Belem, Para, Brazil; Univ Estado Para, Ctr Ciencias Biol & Saude, Belem, Para, Brazil | |
hcfmusp.author.external | BRITO, Arival Cardoso de:Univ Fed Para, NUcleo Med Trop, Belem, Para, Brazil | |
hcfmusp.author.external | NASCIMENTO, Gabriela Yasmin Francisca da Silva do:Univ Sao Paulo, Fac Med, Dept Patol, Av Dr Arnaldo 455,Sale 1118, BR-01246903 Sao Paulo, SP, Brazil; Inst Assistencia Med Servidor Publ Estadual, Programa Posgrad Ciencias Saude, Grad Ciencias SaUde, Sao Paulo, SP, Brazil | |
hcfmusp.description.articlenumber | e75 | |
hcfmusp.description.volume | 62 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000582280200001 | |
hcfmusp.origem.id | 2-s2.0-85092527278 | |
hcfmusp.origem.id | SCIELO:S0036-46652020000100609 | |
hcfmusp.publisher.city | SAO PAULO | eng |
hcfmusp.publisher.country | BRAZIL | eng |
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dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 1678-9946 | |
hcfmusp.citation.scopus | 0 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/06 Artigos e Materiais de Revistas Científicas - LIM/53 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_KANASHIRO-GALO_Lacaziosis_immunohistochemical_evaluatio_of_elements_of_the_humoral_2020.PDF.pdf | publishedVersion (English) | 1.68 MB | Adobe PDF | View/Open |
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