A simplified experimental model of large-for-size liver transplantation in pigs

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author LEAL, Antonio Jose Goncalves FMUSP-HC
TANNURI, Ana Cristina Aoun FMUSP-HC
BELON, Alessandro Rodrigo FMUSP-HC
GUIMARAES, Raimundo Renato Nunes FMUSP-HC
COELHO, Maria Cecilia Mendonca FMUSP-HC
GONCALVES, Josiane de Oliveira FMUSP-HC
SOKOL, Suellen Serafini FMUSP-HC
MELO, Evandro Sobroza De FMUSP-HC
OTOCH, Jose Pinhata FMUSP-HC
TANNURI, Uenis FMUSP-HC
dc.date.issued 2013
dc.identifier.citation CLINICS, v.68, n.8, p.1152-1156, 2013
dc.identifier.issn 1807-5932
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/3868
dc.description.abstract OBJECTIVE: The ideal ratio between liver graft mass and recipient body weight for liver transplantation in small infants is unknown; however, if this ratio is over 4%, a condition called large-for-size may occur. Experimental models of large-for-size liver transplants have not been described in the literature. In addition, orthotopic liver transplantation is marked by high morbidity and mortality rates in animals due to the clamping of the venous splanchnic system. Therefore, the objective of this study was to create a porcine model of large-for-size liver transplantation with clamping of the supraceliac aorta during the anhepatic phase as an alternative to venovenous bypass. METHOD: Fourteen pigs underwent liver transplantation with whole-liver grafts without venovenous bypass and were divided into two experimental groups: the control group, in which the weights of the donors were similar to the weights of the recipients; and the large-for-size group, in which the weights of the donors were nearly 2 times the weights of the recipients. Hemodynamic data, the results of serum biochemical analyses and histological examination of the transplanted livers were collected. RESULTS: The mortality rate in both groups was 16.5% (1/7). The animals in the large-for-size group had increased serum levels of potassium, sodium, aspartate aminotransferase and alanine aminotransferase after graft reperfusion. The histological analyses revealed that there were no significant differences between the groups. CONCLUSION: This transplant method is a feasible experimental model of large-for-size liver transplantation.
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/12550-0]
dc.language.iso eng
dc.publisher HOSPITAL CLINICAS, UNIV SAO PAULO
dc.relation.ispartof Clinics
dc.rights openAccess
dc.subject Experimental Orthotopic Liver Transplantation; Large-For-Size Liver Transplantation; Supraceliac Aortic Liver Transplantation; Anhepatic Phase
dc.subject.other graft; hemodynamics; experience; resection; donors; bypass; repair
dc.title A simplified experimental model of large-for-size liver transplantation in pigs
dc.type article
dc.rights.holder Copyright HOSPITAL CLINICAS, UNIV SAO PAULO
dc.description.group LIM/30
dc.description.group LIM/14
dc.description.group LIM/26
dc.identifier.doi 10.6061/clinics/2013(08)15
dc.identifier.pmid 24037013
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author LEAL, Antonio Jose Goncalves:FM:
hcfmusp.author TANNURI, Ana Cristina Aoun:FM:MCG
hcfmusp.author BELON, Alessandro Rodrigo:FM:
hcfmusp.author GUIMARAES, Raimundo Renato Nunes:HC:ICR
hcfmusp.author COELHO, Maria Cecilia Mendonca:HC:LIM/30
hcfmusp.author GONCALVES, Josiane de Oliveira:FM:
hcfmusp.author SOKOL, Suellen Serafini:HC:LIM/30
hcfmusp.author MELO, Evandro Sobroza De:FM:MPT
hcfmusp.author OTOCH, Jose Pinhata:FM:MCG
hcfmusp.author TANNURI, Uenis:FM:MPE
hcfmusp.origem.id 2-s2.0-84884233107
hcfmusp.origem.id WOS:000324496600015
hcfmusp.origem.id SCIELO:S1807-59322013000801152
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
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dc.description.index MEDLINE
hcfmusp.citation.scopus 9
hcfmusp.citation.wos 10


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