Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/3946
Title: CD4(+) T-cell activation impairs serogroup C Neisseria meningitis vaccine response in HIV-infected children
Authors: MILAGRES, Lucimar G.COSTA, Priscilla R.SANTOS, Bianca A. N.SILVA, Giselle P.CRUZ, Aline C.PEREIRA-MANFRO, Wania F.FERREIRA, BiancaBARRETO, Daniella M.FROTA, Ana Cristina C.KALIL, JorgeHOFER, Cristina B.KALLAS, Esper G.
Citation: AIDS, v.27, n.17, p.2697-2705, 2013
Abstract: Objective:To investigate the influence of CD4(+) T-cell activation and regulatory populations in HIV-infected children antibody response to vaccination with a conjugate C polysaccharide vaccine.Design:CD4(+) T-cell activation was evaluated by expression of CD38, HLA-DR and CCR5 molecules. Regulatory CD4(+) T cells (T-Reg) were characterized as FoxP3(+)CD127(-)CD25(+) and inducer T cells (T-Ind) as CD4(+)FoxP3(-)CD25(-)CD39(+).Methods:All patients (n=36) were HIV-vertically infected, aged 2-17 years-old and were vaccinated with one vaccine injection. Blood samples were obtained before and after immunization to determine bactericidal antibody titers (SBA), CD4(+) T-cell activation and frequency of T-Reg and T-Ind subsets (multiparametric flow cytometry).Results:Children not-responding (n=18) to MenC vaccine expressed higher frequency of activated CD4(+) T cells (HLA-DR(+)CD38(+)CCR5(+)) than responders (n=18), both before and after vaccination (P<0.05). A significant higher frequency of T-Reg was detected in responders compared with nonresponders (P=0.0001). We also detected an inverse correlation between CD4(+)DR(+)CD38(+)CCR5(+) (P=0.01) or CD4(+)DR(+)CD38(+) (P=0.02) T cells and T-Reg cell frequency after vaccination. CD4(+) T-cell activation negatively correlated (P=0.006) with postvaccination SBA titers but a positive correlation (P=0.0001) was detected between T-Reg cells and SBA. T-Reg and T-Ind subsets were inversely correlated (P=0.04).Conclusion:Our findings suggest that higher CD4(+) T-cell activation leads to poor vaccine response in children living with HIV, which may be associated with a T-Reg/T-Ind disequilibrium.
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Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

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Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

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LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular

Artigos e Materiais de Revistas Científicas - LIM/60
LIM/60 - Laboratório de Imunologia Clínica e Alergia


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