Please use this identifier to cite or link to this item:
Title: Transfer of lipids to high-density lipoprotein (HDL) is altered in patients with familial hypercholesterolemia
Authors: MARTINEZ, Lilton R. C.SANTOS, Raul D.MINAME, Marcio H.DEUS, Debora F.LIMA, Emerson S.MARANHAO, Raul C.
Citation: METABOLISM-CLINICAL AND EXPERIMENTAL, v.62, n.8, p.1061-1064, 2013
Abstract: Objective. In familial hypercholesterolemia (FH), the metabolism and anti-atherogenic functions of HDL can be affected by the continuous interactions with excess LDL amounts. Here, lipid transfers to HDL, an important step for HDL intravascular metabolism and for HDL role in reverse cholesterol transport (RCT) were investigated in PH patients. Methods. Seventy-one PH patients (39 +/- 15 years, LDL-cholesterol = 274 +/- 101; HDL-cholesterol = 50 +/- 14 mg/di) and 66 normolipidemic subjects (NL) (38 +/- 11 years, LDL-cholesterol = 105 +/- 27; HDL-cholesterol = 52 +/- 12 mg/dl) were studied. In vitro, lipid transfers were evaluated by incubation of plasma samples (37 degrees C, 1 h) with a donor lipid nanoemulsion labeled with 3H-triglycerides (TG) and 14C-unesterified cholesterol (UC) or with 3H-cholesteryl ester (EC) and 14C-phospholipids (PL). Radioactivity was counted at the HDL fraction after chemical precipitation of apolipoprotein (apo) B-containing lipoproteins and the nanoemulsion. Data are % of total radioactivity measured in the HDL fraction. Results. Transfer of UC to HDL was lower in FH than in NL (5.6 +/- 2.1 vs 6.7 +/- 2.0%, p = 0.0005) whereas TG (5.5 +/- 3.1 vs 3.7 +/- 0.9%, p = 0.018) and PL (20.9 +/- 4.6 vs 18.2 +/- 3.7 %, p = 0.023) transfers were higher in FH. EC transfer was equal. By multivariate analysis, transfers of all four lipids correlated with HDL-cholesterol and with apo A-I. Conclusion. FH elicited marked changes in three of the four tested lipid transfers to HDL. The entry of UC into HDL for subsequent esterification is an important driving force for RCT and reduction of UC transfer to HDL was previously associated to precocious coronary heart disease. Therefore, in FH, HDL functions can be lessened, which can also contribute to atherogenesis.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCP
Departamento de Cardio-Pneumologia - FM/MCP

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/31
LIM/31 - Laboratório de Genética e Hematologia Molecular

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

Files in This Item:
File Description SizeFormat 
  Restricted Access
publishedVersion (English)253.39 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.