Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/40219
Title: | Evaluation of the diaphragm muscle remodeling, inflammation, oxidative stress and vascularization in smokers: An autopsy study |
Authors: | NUCCI, R.A.B.; MAIFRINO, L.B.M.; BUSSE, A.L.; SOUZA, R.R. De; PASQUALUCCI, C.A.; ANARUMA, C.A.; LEITE, R.E.P.; RODRIGUEZ, R.D.; SUEMOTO, C.K.; JACOB-FILHO, W. |
Citation: | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v.54, n.4, p.567-576, 2020 |
Abstract: | Background/Aims: Cigarette smoking is a key factor in systemic inflammation and oxidative stress, and it has also been associated with the loss of muscle strength and an elevated risk of pulmonary diseases. Thus, this study aimed to analyze the effects of cigarette smoking on the diaphragm muscle structure of postmortem samples. Methods: Immunohistochemical techniques were used for muscle remodeling (metalloproteinases 2 and 9), inflammation (cyclooxygenase-2), oxidative stress (8-hydroxy-2'-deoxyguanosine), and vascularization (vascular endothelial growth factor). Hematoxylin and eosin stain was used for histopathological analysis and Picrosirius stain was used to highlight the collagen fibers. Results: Cigarette smokers had an increase of diaphragm muscle remodeling, oxidative stress, inflammation, and vascularization compared to non-smokers. Conclusion: Diaphragm muscle structure may be negatively affected by cigarette smoking. © 2020 The Author(s). |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/22 Artigos e Materiais de Revistas Científicas - LIM/66 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_NUCCI_Evaluation_of_the_diaphragm_muscle_remodeling_inflammation_oxidative_2020.PDF | publishedVersion (English) | 2.16 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.