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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | CAPCHA, Jose Manuel Condor | - |
dc.contributor.author | MOREIRA, Roberto S. | - |
dc.contributor.author | RODRIGUES, Camila E. | - |
dc.contributor.author | SILVEIRA, Marcelo A. D. | - |
dc.contributor.author | ANDRADE, Lucia | - |
dc.contributor.author | GOMES, Samirah A. | - |
dc.date.accessioned | 2021-06-17T13:50:28Z | - |
dc.date.available | 2021-06-17T13:50:28Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | BIO-PROTOCOL, v.11, n.7, article ID e3979, 9p, 2021 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/40531 | - |
dc.description.abstract | Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans. Despite several studies and modified CLP protocols, there are still open questions regarding the multifactorial determinants of its reproducibility and medical significance. In our protocol, which is also aimed at mimicking the sepsis observed in clinical practice, male Wistar rats are submitted to CLP with adequate fluid resuscitation (0.15 M NaCl, 25 ml/kg BW i.p.) immediately after surgery. At 6 h after CLP, additional fluid therapy (0.15 M NaCl, 25 ml/kg BW s.c.) and antibiotic therapy with imipenem-cilastatin (single dose of 14 mg/kg BW s.c.) are administered. The timing of the fluid and antibiotic therapy correspond to the initial care given when patients are admitted to the intensive care unit. This model of sepsis provides a useful platform for simulating human sepsis and could lay the groundwork for the development of new treatments. | eng |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo Research Foundation)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/19012-0] | - |
dc.description.sponsorship | FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/21308-9] | - |
dc.description.sponsorship | Brazilian Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (National Council for Scientific and Technological Development)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [301193/2016-9] | - |
dc.language.iso | eng | - |
dc.publisher | BIO-PROTOCOL | eng |
dc.relation.ispartof | Bio-Protocol | - |
dc.rights | restrictedAccess | eng |
dc.subject | Sepsis | eng |
dc.subject | Rats | eng |
dc.subject | Cecal ligation and puncture (CLP) | eng |
dc.subject | Organ dysfunction | eng |
dc.subject | Animal Model | eng |
dc.subject | Acute Kidney Injury | eng |
dc.title | Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction | eng |
dc.type | article | eng |
dc.rights.holder | Copyright BIO-PROTOCOL | eng |
dc.identifier.doi | 10.21769/BioProtoc.3979 | - |
dc.identifier.pmid | 33889673 | - |
dc.subject.wos | Biology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | CAPCHA, Jose Manuel Condor:Univ Sao Paulo, Sch Med, Lab Basic Res, Sao Paulo, Brazil; Univ Sao Paulo, Sch Med, Lab Genet Cellular Biol & Mol Biol, Sao Paulo, Brazil | - |
hcfmusp.author.external | MOREIRA, Roberto S.:Fed Univ Catalao, Catalao, Brazil | - |
hcfmusp.description.articlenumber | e3979 | - |
hcfmusp.description.issue | 7 | - |
hcfmusp.description.volume | 11 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000637687900014 | - |
hcfmusp.origem.id | 2-s2.0-85114217317 | - |
hcfmusp.publisher.city | SUNNYVALE | eng |
hcfmusp.publisher.country | USA | eng |
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dc.description.index | PubMed | eng |
dc.identifier.eissn | 2331-8325 | - |
hcfmusp.citation.scopus | 2 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/12 Artigos e Materiais de Revistas Científicas - LIM/29 |
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art_CAPCHA_Using_the_Cecal_Ligation_and_Puncture_Model_of_2021.PDF Restricted Access | publishedVersion (English) | 2.98 MB | Adobe PDF | View/Open Request a copy |
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