Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/40754
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | BATISSOCO, A. C. | |
dc.contributor.author | LEZIROVITZ, K. | |
dc.contributor.author | ZANATTA, D. B. | |
dc.contributor.author | HEMZA, C. R. M. L. | |
dc.contributor.author | VASQUES, L. R. | |
dc.contributor.author | STRAUSS, B. E. | |
dc.contributor.author | MINGRONI-NETTO, R. C. | |
dc.contributor.author | HADDAD, L. A. | |
dc.contributor.author | BENTO, R. F. | |
dc.contributor.author | OITICICA, J. | |
dc.date.accessioned | 2021-06-17T13:53:59Z | - |
dc.date.available | 2021-06-17T13:53:59Z | - |
dc.date.issued | 2021 | |
dc.identifier.citation | BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.54, n.7, article ID e10579, 8p, 2021 | |
dc.identifier.issn | 0100-879X | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/40754 | - |
dc.description.abstract | NOTCH pathway proteins, including the transcriptional factor HES1, play crucial roles in the development of the inner ear by means of the lateral inhibition mechanism, in which supporting cells have their phenotype preserved while they are prevented from becoming hair cells. Genetic manipulation of this pathway has been demonstrated to increase hair cell number. The present study aimed to investigate gene expression effects in hair cells and supporting cells after Hes1-shRNA lentivirus transduction in organotypic cultures of the organ of Corti from postnatal-day-3 mice. Forty-eight hours after in vitro knockdown, Hes1 gene expression was reduced at both mRNA and protein levels. Myo7a (hair cell marker) and Sox2 (progenitor cell marker) mRNA levels also significantly increased. The modulation of gene expression in the organ of Corti upon Hes1 knockdown is consistent with cell phenotypes related to lateral inhibition mechanism interference in the inner ear. The lentivirus-based expression of Hes1-sh RNA is a valuable strategy for genetic interference in the organ of Corti and for future evaluation of its efficacy in protocols aiming at the regeneration of hair cells in vivo. | eng |
dc.description.sponsorship | Sao Paulo Research Foundation (FAPESP, Sao Paulo, Brazil)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/08028-1, 2009/09473-3, 2008/00458-9] | |
dc.description.sponsorship | Brazilian National Council for Scientific and Technological Development (CNPq, Brasilia, Brazil)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [573920/2008-7] | |
dc.language.iso | eng | |
dc.publisher | ASSOC BRAS DIVULG CIENTIFICA | eng |
dc.relation.ispartof | Brazilian Journal of Medical and Biological Research | |
dc.rights | openAccess | eng |
dc.subject | shRNA | eng |
dc.subject | Hearing loss | eng |
dc.subject | Inner hair cells | eng |
dc.subject | Organ of Corti | eng |
dc.subject | Hes1 knockdown | eng |
dc.subject | Lentivirus | eng |
dc.subject.other | organ | eng |
dc.subject.other | proliferation | eng |
dc.subject.other | p27(kip1) | eng |
dc.title | Cochlea cell-specific marker expression upon in vitro Hes1 knockdown | eng |
dc.type | article | eng |
dc.rights.holder | Copyright ASSOC BRAS DIVULG CIENTIFICA | eng |
dc.identifier.doi | 10.1590/1414-431X2020e10579 | |
dc.identifier.pmid | 34008754 | |
dc.subject.wos | Biology | eng |
dc.subject.wos | Medicine, Research & Experimental | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | ZANATTA, D. B.:Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo,Lab Vetores Virais, Sao Paulo, Brazil | |
hcfmusp.author.external | VASQUES, L. R.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil | |
hcfmusp.author.external | MINGRONI-NETTO, R. C.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil | |
hcfmusp.author.external | HADDAD, L. A.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil | |
hcfmusp.description.articlenumber | e10579 | |
hcfmusp.description.issue | 7 | |
hcfmusp.description.volume | 54 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000654362900001 | |
hcfmusp.origem.id | 2-s2.0-85106178960 | |
hcfmusp.origem.id | SCIELO:S0100-879X2021000700601 | |
hcfmusp.publisher.city | RIBEIRAO PRETO | eng |
hcfmusp.publisher.country | BRAZIL | eng |
hcfmusp.relation.reference | Abdolazimi Y, 2016, DEVELOPMENT, V143, P841, DOI 10.1242/dev.129320 | eng |
hcfmusp.relation.reference | Atkinson PJ, 2019, EXPERT OPIN BIOL TH, V19, P129, DOI 10.1080/14712598.2019.1564035 | eng |
hcfmusp.relation.reference | Barboza LCM, 2016, BRAZ J MED BIOL RES, V49, DOI 10.1590/1414-431X20155064 | eng |
hcfmusp.relation.reference | Batts SA, 2009, HEARING RES, V249, P15, DOI 10.1016/j.heares.2008.12.008 | eng |
hcfmusp.relation.reference | Chen P, 1999, DEVELOPMENT, V126, P1581 | eng |
hcfmusp.relation.reference | Du XP, 2018, MOL THER, V26, P1313, DOI 10.1016/j.ymthe.2018.03.004 | eng |
hcfmusp.relation.reference | Du XP, 2013, HEARING RES, V304, P91, DOI 10.1016/j.heares.2013.06.011 | eng |
hcfmusp.relation.reference | Kempfle JS, 2016, SCI REP-UK, V6, DOI 10.1038/srep23293 | eng |
hcfmusp.relation.reference | Lanford PJ, 1999, NAT GENET, V21, P289 | eng |
hcfmusp.relation.reference | Maass JC, 2013, JARO-J ASSOC RES OTO, V14, P495, DOI 10.1007/s10162-013-0383-2 | eng |
hcfmusp.relation.reference | Massucci-Bissoli M, 2017, CLINICS, V72, P714, DOI 10.6061/clinics/2017(11)11 | eng |
hcfmusp.relation.reference | Mathers C, GLOBAL BURDEN HEARIN | eng |
hcfmusp.relation.reference | Oesterle EC, 2011, CELL CYCLE, V10, P1237, DOI 10.4161/cc.10.8.15301 | eng |
hcfmusp.relation.reference | Oiticica J, 2010, J TRANSL MED, V8, DOI 10.1186/1479-5876-8-119 | eng |
hcfmusp.relation.reference | Su YX, 2015, GENE, V558, P6, DOI 10.1016/j.gene.2014.12.054 | eng |
hcfmusp.relation.reference | Tateya T, 2011, DEV BIOL, V352, P329, DOI 10.1016/j.ydbio.2011.01.038 | eng |
hcfmusp.relation.reference | Wang B, 2011, ACTA NEUROBIOL EXP, V71, P256 | eng |
hcfmusp.relation.reference | Yamamoto N, 2006, J MOL MED, V84, P37, DOI 10.1007/s00109-005-0706-9 | eng |
hcfmusp.relation.reference | Zheng JL, 2000, DEVELOPMENT, V127, P4551 | eng |
dc.description.index | MEDLINE | eng |
dc.identifier.eissn | 1414-431X | |
hcfmusp.citation.scopus | 0 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MOF Artigos e Materiais de Revistas Científicas - HC/ICESP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/24 Artigos e Materiais de Revistas Científicas - LIM/32 Artigos e Materiais de Revistas Científicas - ODS/03 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_BATISSOCO_Cochlea_cellspecific_marker_expression_upon_in_vitro_Hes1_2021.PDF | publishedVersion (English) | 703.89 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.