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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBATISSOCO, A. C.
dc.contributor.authorLEZIROVITZ, K.
dc.contributor.authorZANATTA, D. B.
dc.contributor.authorHEMZA, C. R. M. L.
dc.contributor.authorVASQUES, L. R.
dc.contributor.authorSTRAUSS, B. E.
dc.contributor.authorMINGRONI-NETTO, R. C.
dc.contributor.authorHADDAD, L. A.
dc.contributor.authorBENTO, R. F.
dc.contributor.authorOITICICA, J.
dc.identifier.citationBRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.54, n.7, article ID e10579, 8p, 2021
dc.description.abstractNOTCH pathway proteins, including the transcriptional factor HES1, play crucial roles in the development of the inner ear by means of the lateral inhibition mechanism, in which supporting cells have their phenotype preserved while they are prevented from becoming hair cells. Genetic manipulation of this pathway has been demonstrated to increase hair cell number. The present study aimed to investigate gene expression effects in hair cells and supporting cells after Hes1-shRNA lentivirus transduction in organotypic cultures of the organ of Corti from postnatal-day-3 mice. Forty-eight hours after in vitro knockdown, Hes1 gene expression was reduced at both mRNA and protein levels. Myo7a (hair cell marker) and Sox2 (progenitor cell marker) mRNA levels also significantly increased. The modulation of gene expression in the organ of Corti upon Hes1 knockdown is consistent with cell phenotypes related to lateral inhibition mechanism interference in the inner ear. The lentivirus-based expression of Hes1-sh RNA is a valuable strategy for genetic interference in the organ of Corti and for future evaluation of its efficacy in protocols aiming at the regeneration of hair cells in vivo.eng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP, Sao Paulo, Brazil)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/08028-1, 2009/09473-3, 2008/00458-9]
dc.description.sponsorshipBrazilian National Council for Scientific and Technological Development (CNPq, Brasilia, Brazil)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [573920/2008-7]
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.subjectHearing losseng
dc.subjectInner hair cellseng
dc.subjectOrgan of Cortieng
dc.subjectHes1 knockdowneng
dc.titleCochlea cell-specific marker expression upon in vitro Hes1 knockdowneng
dc.rights.holderCopyright ASSOC BRAS DIVULG CIENTIFICAeng
dc.subject.wosMedicine, Research & Experimentaleng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, D. B.:Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo,Lab Vetores Virais, Sao Paulo, Brazil, L. R.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil, R. C.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil, L. A.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil
hcfmusp.publisher.cityRIBEIRAO PRETOeng
hcfmusp.relation.referenceAbdolazimi Y, 2016, DEVELOPMENT, V143, P841, DOI 10.1242/dev.129320eng
hcfmusp.relation.referenceAtkinson PJ, 2019, EXPERT OPIN BIOL TH, V19, P129, DOI 10.1080/14712598.2019.1564035eng
hcfmusp.relation.referenceBarboza LCM, 2016, BRAZ J MED BIOL RES, V49, DOI 10.1590/1414-431X20155064eng
hcfmusp.relation.referenceBatts SA, 2009, HEARING RES, V249, P15, DOI 10.1016/j.heares.2008.12.008eng
hcfmusp.relation.referenceChen P, 1999, DEVELOPMENT, V126, P1581eng
hcfmusp.relation.referenceDu XP, 2018, MOL THER, V26, P1313, DOI 10.1016/j.ymthe.2018.03.004eng
hcfmusp.relation.referenceDu XP, 2013, HEARING RES, V304, P91, DOI 10.1016/j.heares.2013.06.011eng
hcfmusp.relation.referenceKempfle JS, 2016, SCI REP-UK, V6, DOI 10.1038/srep23293eng
hcfmusp.relation.referenceLanford PJ, 1999, NAT GENET, V21, P289eng
hcfmusp.relation.referenceMaass JC, 2013, JARO-J ASSOC RES OTO, V14, P495, DOI 10.1007/s10162-013-0383-2eng
hcfmusp.relation.referenceMassucci-Bissoli M, 2017, CLINICS, V72, P714, DOI 10.6061/clinics/2017(11)11eng
hcfmusp.relation.referenceMathers C, GLOBAL BURDEN HEARINeng
hcfmusp.relation.referenceOesterle EC, 2011, CELL CYCLE, V10, P1237, DOI 10.4161/cc.10.8.15301eng
hcfmusp.relation.referenceOiticica J, 2010, J TRANSL MED, V8, DOI 10.1186/1479-5876-8-119eng
hcfmusp.relation.referenceSu YX, 2015, GENE, V558, P6, DOI 10.1016/j.gene.2014.12.054eng
hcfmusp.relation.referenceTateya T, 2011, DEV BIOL, V352, P329, DOI 10.1016/j.ydbio.2011.01.038eng
hcfmusp.relation.referenceWang B, 2011, ACTA NEUROBIOL EXP, V71, P256eng
hcfmusp.relation.referenceYamamoto N, 2006, J MOL MED, V84, P37, DOI 10.1007/s00109-005-0706-9eng
hcfmusp.relation.referenceZheng JL, 2000, DEVELOPMENT, V127, P4551eng
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MOF
Departamento de Otorrinolaringologia e Oftalmologia - FM/MOF

Artigos e Materiais de Revistas Científicas - HC/ICESP
Instituto do Câncer do Estado de São Paulo - HC/ICESP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/24
LIM/24 - Laboratório de Oncologia Experimental

Artigos e Materiais de Revistas Científicas - LIM/32
LIM/32 - Laboratório de Otorrinolaringologia

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