Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/40754
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorBATISSOCO, A. C.
dc.contributor.authorLEZIROVITZ, K.
dc.contributor.authorZANATTA, D. B.
dc.contributor.authorHEMZA, C. R. M. L.
dc.contributor.authorVASQUES, L. R.
dc.contributor.authorSTRAUSS, B. E.
dc.contributor.authorMINGRONI-NETTO, R. C.
dc.contributor.authorHADDAD, L. A.
dc.contributor.authorBENTO, R. F.
dc.contributor.authorOITICICA, J.
dc.date.accessioned2021-06-17T13:53:59Z-
dc.date.available2021-06-17T13:53:59Z-
dc.date.issued2021
dc.identifier.citationBRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.54, n.7, article ID e10579, 8p, 2021
dc.identifier.issn0100-879X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/40754-
dc.description.abstractNOTCH pathway proteins, including the transcriptional factor HES1, play crucial roles in the development of the inner ear by means of the lateral inhibition mechanism, in which supporting cells have their phenotype preserved while they are prevented from becoming hair cells. Genetic manipulation of this pathway has been demonstrated to increase hair cell number. The present study aimed to investigate gene expression effects in hair cells and supporting cells after Hes1-shRNA lentivirus transduction in organotypic cultures of the organ of Corti from postnatal-day-3 mice. Forty-eight hours after in vitro knockdown, Hes1 gene expression was reduced at both mRNA and protein levels. Myo7a (hair cell marker) and Sox2 (progenitor cell marker) mRNA levels also significantly increased. The modulation of gene expression in the organ of Corti upon Hes1 knockdown is consistent with cell phenotypes related to lateral inhibition mechanism interference in the inner ear. The lentivirus-based expression of Hes1-sh RNA is a valuable strategy for genetic interference in the organ of Corti and for future evaluation of its efficacy in protocols aiming at the regeneration of hair cells in vivo.eng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP, Sao Paulo, Brazil)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/08028-1, 2009/09473-3, 2008/00458-9]
dc.description.sponsorshipBrazilian National Council for Scientific and Technological Development (CNPq, Brasilia, Brazil)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [573920/2008-7]
dc.language.isoeng
dc.publisherASSOC BRAS DIVULG CIENTIFICAeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsopenAccesseng
dc.subjectshRNAeng
dc.subjectHearing losseng
dc.subjectInner hair cellseng
dc.subjectOrgan of Cortieng
dc.subjectHes1 knockdowneng
dc.subjectLentiviruseng
dc.subject.otherorganeng
dc.subject.otherproliferationeng
dc.subject.otherp27(kip1)eng
dc.titleCochlea cell-specific marker expression upon in vitro Hes1 knockdowneng
dc.typearticleeng
dc.rights.holderCopyright ASSOC BRAS DIVULG CIENTIFICAeng
dc.identifier.doi10.1590/1414-431X2020e10579
dc.identifier.pmid34008754
dc.subject.wosBiologyeng
dc.subject.wosMedicine, Research & Experimentaleng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalZANATTA, D. B.:Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol LIM24, Inst Canc Estado Sao Paulo,Lab Vetores Virais, Sao Paulo, Brazil
hcfmusp.author.externalVASQUES, L. R.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil
hcfmusp.author.externalMINGRONI-NETTO, R. C.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil
hcfmusp.author.externalHADDAD, L. A.:Univ Sao Paulo, Ctr Estudos Genoma Humano & Celulas Tronco, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP, Brazil
hcfmusp.description.articlenumbere10579
hcfmusp.description.issue7
hcfmusp.description.volume54
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000654362900001
hcfmusp.origem.id2-s2.0-85106178960
hcfmusp.origem.idSCIELO:S0100-879X2021000700601
hcfmusp.publisher.cityRIBEIRAO PRETOeng
hcfmusp.publisher.countryBRAZILeng
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dc.description.indexMEDLINEeng
dc.identifier.eissn1414-431X
hcfmusp.citation.scopus0-
hcfmusp.scopus.lastupdate2022-09-06-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MOF
Departamento de Otorrinolaringologia e Oftalmologia - FM/MOF

Artigos e Materiais de Revistas Científicas - HC/ICESP
Instituto do Câncer do Estado de São Paulo - HC/ICESP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/24
LIM/24 - Laboratório de Oncologia Experimental

Artigos e Materiais de Revistas Científicas - LIM/32
LIM/32 - Laboratório de Otorrinolaringologia


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