Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/4076
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorSATOW, Marcela M.-
dc.contributor.authorYAMASHIRO-KANASHIRO, Edite H.-
dc.contributor.authorROCHA, Mussya C.-
dc.contributor.authorOYAFUSO, Luiza K.-
dc.contributor.authorSOLER, Rita C.-
dc.contributor.authorCOTRIM, Paulo C.-
dc.contributor.authorLINDOSO, Jose Angelo L.-
dc.date.accessioned2014-01-28T22:22:10Z-
dc.date.available2014-01-28T22:22:10Z-
dc.date.issued2013-
dc.identifier.citationREVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.55, n.6, p.393-399, 2013-
dc.identifier.issn0036-4665-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/4076-
dc.description.abstractThis study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emolio Ribas (IIER), a reference center for infectious diseases in Sao Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER.-
dc.description.abstractEste estudo avaliou a aplicabilidade do kDNA-PCR como método de rotina para diagnóstico de leishmaniose tegumentar americana (ATL) no Instituto de Infectologia Emílio Ribas (IIER), São Paulo, SP, Brasil. O método kDNA-PCR detectou DNA de Leishmania em 87,5% (112/128) dos pacientes com suspeita de ter leishmaniose e, os métodos tradicionais apresentaram as seguintes porcentagens de positividade: 62,8% (49/78) para o teste de Montenegro, 61,8% (47/76) para a pesquisa direta e 19,3% (22/114) para cultura in vitro. O método molecular confirmou a doença em amostras negativas ou inconclusivas pelos métodos laboratoriais tradicionais e, mostrou-se capaz de auxiliar na identificação de infecções causadas pela espécie Leishmania (V.) braziliensis. Além disso, a revisão dos prontuários médicos confirmou a importância do método PCR-RFLP no diagnóstico final de ATL, prognóstico e escolha do tratamento. Assim, recomendamos a inclusão do PCR como método diagnóstico de ATL na rotina hospitalar, e sugerimos um fluxograma para solicitação de exames laboratoriais-
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2010/16963-4]-
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)-
dc.description.sponsorshipCNPq-
dc.description.sponsorshipLaboratorio de Investigacao Medica [FMUSP-HC/LIM-38 e LIM-48]-
dc.language.isoeng-
dc.publisherINST MEDICINA TROPICAL SAO PAULO-
dc.relation.ispartofRevista do Instituto de Medicina Tropical de Sao Paulo-
dc.rightsopenAccess-
dc.subjectAmerican tegumentary leishmaniasis-
dc.subjectDiagnostic-
dc.subjectkDNA-PCR-
dc.subjectLeishmania (Viannia) braziliensis-
dc.subject.otherpolymerase-chain-reaction-
dc.subject.othercutaneous leishmaniasis-
dc.subject.othermucocutaneous leishmaniasis-
dc.subject.otherviannia braziliensis-
dc.subject.otheridentification-
dc.subject.otherperformance-
dc.subject.otherargentina-
dc.titleAPPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL-
dc.title.alternativeAplicação do kDNA-PCR para diagnóstico de rotina de leishmaniose tegumentar americana em um hospital de referência-
dc.typearticle-
dc.rights.holderCopyright INST MEDICINA TROPICAL SAO PAULO-
dc.identifier.doi10.1590/S0036-46652013000600004-
dc.identifier.pmid24213191-
dc.subject.wosTropical Medicine-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalOYAFUSO, Luiza K.:Inst Infectol Emilio Ribas, Sao Paulo, Brazil-
hcfmusp.author.externalSOLER, Rita C.:Inst Infectol Emilio Ribas, Sao Paulo, Brazil-
hcfmusp.description.beginpage393-
hcfmusp.description.endpage399-
hcfmusp.description.issue6-
hcfmusp.description.volume55-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:000326974700004-
hcfmusp.origem.id2-s2.0-84887335021-
hcfmusp.origem.idSCIELO:S0036-46652013000600393-
hcfmusp.publisher.citySAO PAULO-
hcfmusp.publisher.countryBRAZIL-
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dc.description.indexMEDLINE-
hcfmusp.remissive.sponsorshipCAPES-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.remissive.sponsorshipFAPESP-
hcfmusp.remissive.sponsorshipFMUSP-HC-
hcfmusp.citation.scopus14-
hcfmusp.scopus.lastupdate2022-06-30-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/38
LIM/38 - Laboratório de Epidemiologia e Imunobiologia

Artigos e Materiais de Revistas Científicas - LIM/48
LIM/48 - Laboratório de Imunologia


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