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Title: A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease
Authors: TEIXEIRA, Andre Azevedo ReisCARNERO, Luis RodriguezKURAMOTO, AndreiaTANG, Fenny Hui FenGOMES, Carlos HerniquePEREIRA, Natalia BuenoOLIVEIRA, Lea Campos deGARRINI, ReginaMONTEIRO, Jhonatas SirinoSETUBAL, Joao CarlosSABINO, Ester CerdeiraPASQUALINI, RenataCOLLI, WalterARAP, WadihALVES, Maria Julia MansoCUNHA-NETO, EdecioGIORDANO, Ricardo Jose
Citation: ISCIENCE, v.24, n.6, article ID 102540, 26p, 2021
Abstract: Large-scale mapping of antigens and epitopes is pivotal for developing immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes. Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasite Trypanosoma cruzi enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show howlinear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.
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Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/03
LIM/03 - Laboratório de Medicina Laboratorial

Artigos e Materiais de Revistas Científicas - LIM/19
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular

Artigos e Materiais de Revistas Científicas - LIM/46
LIM/46 - Laboratório de Parasitologia Médica

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