Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/41453
Full metadata record
DC FieldValueLanguage
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTEIXEIRA, Andre Azevedo Reis
dc.contributor.authorCARNERO, Luis Rodriguez
dc.contributor.authorKURAMOTO, Andreia
dc.contributor.authorTANG, Fenny Hui Fen
dc.contributor.authorGOMES, Carlos Hernique
dc.contributor.authorPEREIRA, Natalia Bueno
dc.contributor.authorOLIVEIRA, Lea Campos de
dc.contributor.authorGARRINI, Regina
dc.contributor.authorMONTEIRO, Jhonatas Sirino
dc.contributor.authorSETUBAL, Joao Carlos
dc.contributor.authorSABINO, Ester Cerdeira
dc.contributor.authorPASQUALINI, Renata
dc.contributor.authorCOLLI, Walter
dc.contributor.authorARAP, Wadih
dc.contributor.authorALVES, Maria Julia Manso
dc.contributor.authorCUNHA-NETO, Edecio
dc.contributor.authorGIORDANO, Ricardo Jose
dc.date.accessioned2021-08-13T15:13:45Z-
dc.date.available2021-08-13T15:13:45Z-
dc.date.issued2021
dc.identifier.citationISCIENCE, v.24, n.6, article ID 102540, 26p, 2021
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/41453-
dc.description.abstractLarge-scale mapping of antigens and epitopes is pivotal for developing immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes. Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasite Trypanosoma cruzi enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show howlinear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.eng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/22.645-1, 2014/21177-9, 2013/16.478-7, 2016/14.506-1, 2012/13.967-4, 2016/06.069-0]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [307412/2017-2]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
dc.language.isoeng
dc.publisherCELL PRESSeng
dc.relation.ispartofIscience
dc.rightsopenAccesseng
dc.subject.othermultiple sequence alignmenteng
dc.subject.othertrypanosoma-cruzieng
dc.subject.othertrans-sialidaseeng
dc.subject.othercardiac myosineng
dc.subject.otherfollow-upeng
dc.subject.otherantigeneng
dc.subject.otherproteineng
dc.subject.otherepitopeseng
dc.subject.otherglycoproteineng
dc.subject.otherperformanceeng
dc.titleA refined genome phage display methodology delineates the human antibody response in patients with Chagas diseaseeng
dc.typearticleeng
dc.rights.holderCopyright CELL PRESSeng
dc.identifier.doi10.1016/j.isci.2021.102540
dc.identifier.pmid34142048
dc.subject.wosMultidisciplinary Scienceseng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalTEIXEIRA, Andre Azevedo Reis:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; Specifica Inc, Los Alamos, NM USA
hcfmusp.author.externalCARNERO, Luis Rodriguez:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalTANG, Fenny Hui Fen:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; Rutgers New Jersey Med Sch, Dept Radiat Oncol, Div Canc Biol, Newark, NJ 07103 USA
hcfmusp.author.externalGOMES, Carlos Hernique:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalGARRINI, Regina:Univ Sao Paulo, Inst Trop Med, Sch Med, BR-05403000 Sao Paulo, SP, Brazil
hcfmusp.author.externalMONTEIRO, Jhonatas Sirino:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalSETUBAL, Joao Carlos:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalPASQUALINI, Renata:Rutgers Canc Inst New Jersey, Newark, NJ 07103 USA; Rutgers New Jersey Med Sch, Dept Radiat Oncol, Div Canc Biol, Newark, NJ 07103 USA
hcfmusp.author.externalCOLLI, Walter:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalARAP, Wadih:Rutgers Canc Inst New Jersey, Newark, NJ 07103 USA; Rutgers New Jersey Med Sch, Dept Med, Div Hematol Oncol, Newark, NJ 07103 USA
hcfmusp.author.externalALVES, Maria Julia Manso:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil
hcfmusp.author.externalGIORDANO, Ricardo Jose:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; INCT, Inst Invest Immunol 3, Sao Paulo, SP, Brazil
hcfmusp.description.articlenumber102540
hcfmusp.description.issue6
hcfmusp.description.volume24
hcfmusp.origemWOS
hcfmusp.origem.idWOS:000667301700042
hcfmusp.origem.id2-s2.0-85109013606
hcfmusp.publisher.cityCAMBRIDGEeng
hcfmusp.publisher.countryUSAeng
hcfmusp.relation.referenceAlvarez P, 2001, INFECT IMMUN, V69, P7946, DOI 10.1128/IAI.69.12.7946-7949.2001eng
hcfmusp.relation.referenceALVES MJM, 1986, MOL BIOCHEM PARASIT, V21, P75, DOI 10.1016/0166-6851(86)90081-2eng
hcfmusp.relation.referenceArap W, 2002, NAT MED, V8, P121, DOI 10.1038/nm0202-121eng
hcfmusp.relation.referenceBeppler J, 2016, EUR J IMMUNOL, V46, P1926, DOI 10.1002/eji.201546118eng
hcfmusp.relation.referenceBergami PL, 2001, FASEB J, V15, P2602, DOI 10.1096/fj.01-0132comeng
hcfmusp.relation.referenceBern C, 2015, NEW ENGL J MED, V373, P456, DOI [10.1056/NEJMra1410150, 10.1056/NEJMc1510996]eng
hcfmusp.relation.referenceBerna L, 2018, MICROB GENOMICS, V4, DOI 10.1099/mgen.0.000177eng
hcfmusp.relation.referenceBertocchi GL, 2013, T ROY SOC TROP MED H, V107, P372, DOI 10.1093/trstmh/trt029eng
hcfmusp.relation.referenceBonney KM, 2019, ANNU REV PATHOL-MECH, V14, P421, DOI 10.1146/annurev-pathol-020117-043711eng
hcfmusp.relation.referenceBuss LF, 2020, PLOS NEGLECT TROP D, V14, DOI 10.1371/journal.pntd.0008787eng
hcfmusp.relation.referenceCaceres DH, 2019, J FUNGI, V5, DOI 10.3390/jof5040111eng
hcfmusp.relation.referenceCamacho C, 2009, BMC BIOINFORMATICS, V10, DOI 10.1186/1471-2105-10-421eng
hcfmusp.relation.referenceCampo V, 2004, MOL BIOCHEM PARASIT, V133, P81, DOI 10.1016/j.molbiopara.2003.09.006eng
hcfmusp.relation.referenceCarmona SJ, 2015, MOL CELL PROTEOMICS, V14, P1871, DOI 10.1074/mcp.M114.045906eng
hcfmusp.relation.referenceChing ATC, 2012, BIOCHEM BIOPH RES CO, V427, P774, DOI 10.1016/j.bbrc.2012.09.137eng
hcfmusp.relation.referenceCOLLI W, 1993, FASEB J, V7, P1257eng
hcfmusp.relation.referenceCunha-Neto E, 2014, MEDIAT INFLAMM, V2014, DOI 10.1155/2014/683230eng
hcfmusp.relation.referenceCunha-Neto E, 2011, ADV PARASIT, V76, P129, DOI 10.1016/B978-0-12-385895-5.00006-2eng
hcfmusp.relation.referenceCUNHANETO E, 1995, P NATL ACAD SCI USA, V92, P3541, DOI 10.1073/pnas.92.8.3541eng
hcfmusp.relation.referenceDe Pablos L.M., 2016, SCI REP-UK, V6, P1eng
hcfmusp.relation.referenceDias-Neto E, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0008338eng
hcfmusp.relation.referenceDurante IM, 2017, PLOS NEGLECT TROP D, V11, DOI 10.1371/journal.pntd.0005986eng
hcfmusp.relation.referenceDuranti MA, 1999, EXP PARASITOL, V93, P38, DOI 10.1006/expr.1999.4428eng
hcfmusp.relation.referenceEdgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340eng
hcfmusp.relation.referenceEL-Manzalawy Y, 2017, METHODS MOL BIOL, V1484, P255, DOI 10.1007/978-1-4939-6406-2_17eng
hcfmusp.relation.referenceEl-Sayed NM, 2005, SCIENCE, V309, P409, DOI 10.1126/science.1112631eng
hcfmusp.relation.referenceEllis S.E., 2012, PARASITE IMMUNOL, V34, P285, DOI 10.1111/j.1365-3024.2011.01317.xeng
hcfmusp.relation.referenceFinn RD, 2014, NUCLEIC ACIDS RES, V42, pD222, DOI [10.1093/nar/gkt263, 10.1093/nar/gkt1223]eng
hcfmusp.relation.referenceFrancolino Sonia S., 2003, Rev. Soc. Bras. Med. Trop., V36, P103, DOI 10.1590/S0037-86822003000100014eng
hcfmusp.relation.referenceFreire-de-Lima L, 2015, GLYCOBIOLOGY, V25, P1142, DOI 10.1093/glycob/cwv057eng
hcfmusp.relation.referenceFreitas LM, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0025914eng
hcfmusp.relation.referenceAlvarez MG, 2016, ANTIMICROB AGENTS CH, V60, P833, DOI 10.1128/AAC.00745-15eng
hcfmusp.relation.referenceGardner MJ, 2002, NATURE, V419, P498, DOI 10.1038/nature01097eng
hcfmusp.relation.referenceGiordanengo L, 2000, EUR J IMMUNOL, V30, P3181, DOI 10.1002/1521-4141(200011)30:11<3181::AID-IMMU3181>3.0.CO;2-Aeng
hcfmusp.relation.referenceGiordano R, 1999, J BIOL CHEM, V274, P3461, DOI 10.1074/jbc.274.6.3461eng
hcfmusp.relation.referenceGiordano RJ, 2001, NAT MED, V7, P1249, DOI 10.1038/nm1101-1249eng
hcfmusp.relation.referenceGoin JC, 1997, FASEB J, V11, P77eng
hcfmusp.relation.referenceGoto Y, 2008, INFECT IMMUN, V76, P3967, DOI 10.1128/IAI.00604-08eng
hcfmusp.relation.referenceGoto Y, 2010, INFECT IMMUN, V78, P2138, DOI 10.1128/IAI.01102-09eng
hcfmusp.relation.referenceHowick VM, 2019, SCIENCE, V365, P774, DOI 10.1126/science.aaw2619eng
hcfmusp.relation.referenceIBANEZ CF, 1988, MOL BIOCHEM PARASIT, V30, P27, DOI 10.1016/0166-6851(88)90129-6eng
hcfmusp.relation.referenceJacobsson Karin, 2003, Biological Procedures Online, V5, P123, DOI 10.1251/bpo54eng
hcfmusp.relation.referenceKamath K, 2020, SCI REP-UK, V10, DOI 10.1038/s41598-020-62256-9eng
hcfmusp.relation.referenceKaplan D, 1997, P NATL ACAD SCI USA, V94, P10301, DOI 10.1073/pnas.94.19.10301eng
hcfmusp.relation.referenceKatoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010eng
hcfmusp.relation.referenceKim YH, 2019, KOREAN J PARASITOL, V57, P435, DOI 10.3347/kjp.2019.57.4.435eng
hcfmusp.relation.referenceLevenshtein VL., 1966, SOV PHYS DOKL, V10, P707eng
hcfmusp.relation.referenceLEVIN MJ, 1993, FEMS IMMUNOL MED MIC, V7, P205, DOI 10.1111/j.1574-695X.1993.tb00400.xeng
hcfmusp.relation.referenceLukes J, 2005, CURR GENET, V48, P277, DOI 10.1007/s00294-005-0027-0eng
hcfmusp.relation.referenceMarin JA, 2007, CIRCULATION, V115, P1109, DOI 10.1161/CIRCULATIONAHA.106.624296eng
hcfmusp.relation.referenceMattos Eliciane C, 2014, Subcell Biochem, V74, P151, DOI 10.1007/978-94-007-7305-9_7eng
hcfmusp.relation.referenceMichaloski JS, 2016, SCI ADV, V2, DOI 10.1126/sciadv.1600611eng
hcfmusp.relation.referenceDe Pablos LM, 2012, INFECT IMMUN, V80, P2258, DOI 10.1128/IAI.06225-11eng
hcfmusp.relation.referenceMintz PJ, 2015, P NATL ACAD SCI USA, V112, P2515, DOI 10.1073/pnas.1500097112eng
hcfmusp.relation.referenceMitrovich QM, 2007, GENOME RES, V17, P492, DOI 10.1101/gr.6111907eng
hcfmusp.relation.referenceMotran CC, 2000, CLIN IMMUNOL, V97, P89, DOI 10.1006/clim.2000.4919eng
hcfmusp.relation.referenceNewman AM, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-382eng
hcfmusp.relation.referenceNormier G., 2007, DEV BIOL STAND, V77, P79eng
hcfmusp.relation.referencePARKER JMR, 1986, BIOCHEMISTRY-US, V25, P5425, DOI 10.1021/bi00367a013eng
hcfmusp.relation.referencePerez-Molina JA, 2018, LANCET, V391, P82, DOI 10.1016/S0140-6736(17)31612-4eng
hcfmusp.relation.referencePETERSON DS, 1986, NATURE, V322, P566, DOI 10.1038/322566a0eng
hcfmusp.relation.referencePettersen EF, 2004, J COMPUT CHEM, V25, P1605, DOI 10.1002/jcc.20084eng
hcfmusp.relation.referencePitcovsky TA, 2002, J INFECT DIS, V186, P397, DOI 10.1086/341463eng
hcfmusp.relation.referencePitcovsky TA, 2001, INFECT IMMUN, V69, P1869, DOI 10.1128/IAI.69.3.1869-1875.2001eng
hcfmusp.relation.referencePollevick GD, 2000, J BIOL CHEM, V275, P27671eng
hcfmusp.relation.referenceRamos CAN, 2009, MEM I OSWALDO CRUZ, V104, P998, DOI 10.1590/S0074-02762009000700010eng
hcfmusp.relation.referenceTeixeira AAR, 2015, PLOS NEGLECT TROP D, V9, DOI 10.1371/journal.pntd.0004099eng
hcfmusp.relation.referenceSabino EC, 2013, TRANSFUSION, V53, P1257, DOI 10.1111/j.1537-2995.2012.03902.xeng
hcfmusp.relation.referenceSAHIN U, 1995, P NATL ACAD SCI USA, V92, P11810, DOI 10.1073/pnas.92.25.11810eng
hcfmusp.relation.referenceFaulin TDS, 2019, BIOMOLECULES, V9, DOI 10.3390/biom9080386eng
hcfmusp.relation.referenceSatoh K, 2009, MICROBIOL IMMUNOL, V53, P41, DOI 10.1111/j.1348-0421.2008.00083.xeng
hcfmusp.relation.referenceSmith GP, 2019, ANGEW CHEM INT EDIT, V58, P14428, DOI 10.1002/anie.201908308eng
hcfmusp.relation.referenceSMITH GP, 1985, SCIENCE, V228, P1315, DOI 10.1126/science.4001944eng
hcfmusp.relation.referenceSutandy F.X., 2013, CURR PROTOC PROTEIN, V27, DOI 10.1002/0471140864.PS270S72eng
hcfmusp.relation.referenceTang FHF, 2019, P NATL ACAD SCI USA, V116, P2300, DOI 10.1073/pnas.1809483116eng
hcfmusp.relation.referenceTarleton RL, 2001, INT J PARASITOL, V31, P550, DOI 10.1016/S0020-7519(01)00158-8eng
hcfmusp.relation.referenceValencia-Hernandez AM, 2020, CELL HOST MICROBE, V27, P950, DOI 10.1016/j.chom.2020.04.010eng
hcfmusp.relation.referenceValiente-Gabioud AA, 2011, EXP PARASITOL, V127, P672, DOI 10.1016/j.exppara.2010.11.011eng
hcfmusp.relation.referenceWaterhouse AM, 2009, BIOINFORMATICS, V25, P1189, DOI 10.1093/bioinformatics/btp033eng
hcfmusp.relation.referenceXu GJ, 2015, SCIENCE, V348, DOI 10.1126/science.aaa0698eng
hcfmusp.relation.referenceZhang LH, 1999, MICROBIOL-UK, V145, P177, DOI 10.1099/13500872-145-1-177eng
hcfmusp.relation.referenceZhang Y, 2004, PROTEINS, V57, P702, DOI 10.1002/prot.20264eng
hcfmusp.relation.referenceZrein M, 2018, PLOS NEGLECT TROP D, V12, DOI 10.1371/journal.pntd.0006226eng
dc.description.indexPubMedeng
dc.identifier.eissn2589-0042
hcfmusp.citation.scopus2-
hcfmusp.scopus.lastupdate2022-06-16-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/03
LIM/03 - Laboratório de Medicina Laboratorial

Artigos e Materiais de Revistas Científicas - LIM/19
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular

Artigos e Materiais de Revistas Científicas - LIM/46
LIM/46 - Laboratório de Parasitologia Médica


Files in This Item:
File Description SizeFormat 
art_TEIXEIRA_A_refined_genome_phage_display_methodology_delineates_the_2021.PDFpublishedVersion (English)8.53 MBAdobe PDFThumbnail
View/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.