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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTEIXEIRA, Andre Azevedo Reis
dc.contributor.authorCARNERO, Luis Rodriguez
dc.contributor.authorKURAMOTO, Andreia
dc.contributor.authorTANG, Fenny Hui Fen
dc.contributor.authorGOMES, Carlos Hernique
dc.contributor.authorPEREIRA, Natalia Bueno
dc.contributor.authorOLIVEIRA, Lea Campos de
dc.contributor.authorGARRINI, Regina
dc.contributor.authorMONTEIRO, Jhonatas Sirino
dc.contributor.authorSETUBAL, Joao Carlos
dc.contributor.authorSABINO, Ester Cerdeira
dc.contributor.authorPASQUALINI, Renata
dc.contributor.authorCOLLI, Walter
dc.contributor.authorARAP, Wadih
dc.contributor.authorALVES, Maria Julia Manso
dc.contributor.authorCUNHA-NETO, Edecio
dc.contributor.authorGIORDANO, Ricardo Jose
dc.identifier.citationISCIENCE, v.24, n.6, article ID 102540, 26p, 2021
dc.description.abstractLarge-scale mapping of antigens and epitopes is pivotal for developing immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes. Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasite Trypanosoma cruzi enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease. Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection. We built and validated a comprehensive map of Chagas disease antibody response to show howlinear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against protein domains that mediate infection pathogenesis. Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.eng
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/22.645-1, 2014/21177-9, 2013/16.478-7, 2016/14.506-1, 2012/13.967-4, 2016/06.069-0]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [307412/2017-2]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
dc.publisherCELL PRESSeng
dc.subject.othermultiple sequence alignmenteng
dc.subject.othercardiac myosineng
dc.titleA refined genome phage display methodology delineates the human antibody response in patients with Chagas diseaseeng
dc.rights.holderCopyright CELL PRESSeng
dc.subject.wosMultidisciplinary Scienceseng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, Andre Azevedo Reis:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; Specifica Inc, Los Alamos, NM USA, Luis Rodriguez:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Fenny Hui Fen:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; Rutgers New Jersey Med Sch, Dept Radiat Oncol, Div Canc Biol, Newark, NJ 07103 USA, Carlos Hernique:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Regina:Univ Sao Paulo, Inst Trop Med, Sch Med, BR-05403000 Sao Paulo, SP, Brazil, Jhonatas Sirino:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Joao Carlos:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Renata:Rutgers Canc Inst New Jersey, Newark, NJ 07103 USA; Rutgers New Jersey Med Sch, Dept Radiat Oncol, Div Canc Biol, Newark, NJ 07103 USA, Walter:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Wadih:Rutgers Canc Inst New Jersey, Newark, NJ 07103 USA; Rutgers New Jersey Med Sch, Dept Med, Div Hematol Oncol, Newark, NJ 07103 USA, Maria Julia Manso:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil, Ricardo Jose:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP, Brazil; INCT, Inst Invest Immunol 3, Sao Paulo, SP, Brazil
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Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

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Instituto do Coração - HC/InCor

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LIM/03 - Laboratório de Medicina Laboratorial

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