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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorGOMES, Claudia-
dc.contributor.authorMERLINI, Mariana-
dc.contributor.authorKONHEIM, Jeremy-
dc.contributor.authorSERAFINI, Paulo-
dc.contributor.authorMOTTA, Eduardo L. A.-
dc.contributor.authorBARACAT, Edmund C.-
dc.contributor.authorSMITH, Gary D.-
dc.identifier.citationFERTILITY AND STERILITY, v.97, n.3, p.714-719, 2012-
dc.description.abstractObjective: To compare the polymerization status of mouse oocyte spindles exposed to various temperatures at various stages of meiosis. Design: Experimental animal study. Setting: University animal laboratory. Animal(s): CF1 mice. Intervention(s): Immature oocytes matured to metaphase I (MI), telophase I (TI), and metaphase II (MII) were incubated at 37 degrees C (control), room temperature (RT), or 4 degrees C for 0, 10, 30, and 60 minutes. Spindle analysis subsequently was performed using polarized field microscopy and immunocytochemistry. Spindles of TI and MII oocytes that underwent vitrification and warming were analyzed also by immunocytochemistry. Main Outcome Measure(s): Detection of polymerized meiotic spindles. Result(s): At RT, and after 60 minutes at 4 degrees C, a significant time-dependent decrease in the percentage of polymerized meiotic spindles was observed in MI and MII oocytes, but not in TI oocytes. The polymerization of TI spindles at 4 degrees C was similar to that of TI spindles at 4 degrees C that underwent vitrification and warming. Conclusion(s): Significant differences in the microtubule dynamics of MI, TI, and MII oocytes incubated at different temperatures were observed. In particular, meiotic spindles in TI oocytes exhibited less depolymerization than did metaphase spindles. (Fertil Steril (R) 2012; 97: 714-9. (C) 2012 by American Society for Reproductive Medicine.)-
dc.relation.ispartofFertility and Sterility-
dc.subjectmeiotic stage-
dc.subject.othermatured human oocytes-
dc.subject.otherliving human oocytes-
dc.subject.otherlight microscopy-
dc.subject.othermouse oocytes-
dc.titleOocyte meiotic-stage-specific differences in spindle depolymerization in response to temperature changes monitored with polarized field microscopy and immunocytochemistry-
dc.rights.holderCopyright ELSEVIER SCIENCE INC-
dc.subject.wosObstetrics & Gynecology-
dc.subject.wosReproductive Biology-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-, Claudia:Univ Sao Paulo, Sch Med, Dept Gynecol, Sao Paulo, Brazil; Huntington Ctr Reprod Med Brazil, Sao Paulo, Brazil-, Mariana:Univ Sao Paulo, Sch Med, Dept Gynecol, Sao Paulo, Brazil-, Jeremy:Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA-, Eduardo L. A.:Huntington Ctr Reprod Med Brazil, Sao Paulo, Brazil; Fed Univ Brazil, Dept Gynecol, Sao Paulo, Brazil-, Gary D.:Univ Sao Paulo, Sch Med, Dept Gynecol, Sao Paulo, Brazil; Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA; Univ Michigan, Dept Urol, Ann Arbor, MI 48109 USA; Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA; Univ Michigan, Reprod Sci Program, Ann Arbor, MI 48109 USA-
hcfmusp.publisher.cityNEW YORK-
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Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MOG
Departamento de Obstetrícia e Ginecologia - FM/MOG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/58
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular

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